Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method, equipment and application of prebiotic composition capable of efficiently regulating intestinal flora

A technology for regulating intestinal flora and prebiotics, which is applied in the directions of non-active components of polymer compounds, medical preparations with non-active components, and medical preparations containing active components, to overcome limitations and achieve good intestinal regulation. , the effect of low cost

Pending Publication Date: 2022-04-01
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no prebiotic composition product that fully utilizes the properties of galactomannan to meet the needs of efficiently regulating the human intestinal flora and improving human intestinal function

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method, equipment and application of prebiotic composition capable of efficiently regulating intestinal flora
  • Preparation method, equipment and application of prebiotic composition capable of efficiently regulating intestinal flora
  • Preparation method, equipment and application of prebiotic composition capable of efficiently regulating intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0099] A method for preparing a prebiotic composition for efficiently regulating intestinal flora, comprising the following steps,

[0100] (S01) Select guar beans, hydrolyze the guar beans in the presence of enzymes and at a temperature of 35-60°C, and after the hydrolysis is completed, perform ultrafiltration and freeze-drying on the hydrolyzed products to obtain partially hydrolyzed guar gum product;

[0101] (S02) Select the gene of mannanase derived from Rhizomucor miehei CAU432, and transfer the gene into Pichia pastoris GS115 for fermentation. When the enzyme activity reaches 72626U / mL, β-mannan is obtained enzyme;

[0102] (S03) adding β-mannanase to the partially hydrolyzed guar gum product for enzymolysis, and after the enzymolysis is completed, the enzymolysis product is subjected to ultrafiltration and freeze-drying to obtain galactomannan;

[0103] (S04) Weigh an appropriate amount of galactomannans, fucoidan oligosaccharides, chitooligosaccharides, galactooligo...

Embodiment 1

[0136] The prebiotic composition for efficiently regulating intestinal flora comprises the following components in parts by weight,

[0137] 20 parts of galactomannans, 15 parts of fucoidan oligosaccharides, 15 parts of chitooligosaccharides, 5 parts of galactooligosaccharides, 5 parts of complex lactobacilli, 10 parts of bifidobacteria, and 15 parts of protective agents;

[0138] 2 parts of Lactobacillus plantarum, 2 parts of Lactobacillus acidophilus, 1 part of Bacillus bulgaricus;

[0139] 5 parts of tryptone, 3 parts of yeast extract, 1 part of L-cysteine, 1 part of heme, 1 part of sodium chloride, 1 part of calcium chloride, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate, vitamin 0.5 parts, resazurin 0.5 parts.

Embodiment 2

[0141] The prebiotic composition for efficiently regulating intestinal flora comprises the following components in parts by weight,

[0142] 30 parts of galactomannans, 20 parts of fucoidan oligosaccharides, 20 parts of chitooligosaccharides, 10 parts of galactooligosaccharides, 10 parts of compound lactobacillus, 15 parts of bifidobacteria, 20 parts of protective agent;

[0143] 4 parts of Lactobacillus plantarum, 4 parts of Lactobacillus acidophilus, 2 parts of Bacillus bulgaricus;

[0144] 8 parts of tryptone, 3 parts of yeast extract, 2 parts of L-cysteine, 1 part of heme, 1 part of sodium chloride, 1 part of calcium chloride, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate, vitamin 1.5 parts, resazurin 0.5 parts.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of prebiotic composition preparation, and particularly discloses a preparation method, equipment and application of a prebiotic composition capable of efficiently adjusting intestinal flora, and the prebiotic composition comprises the following components in parts by weight: 20-30 parts of galactomannan, 15-20 parts of alginate oligosaccharide, 15-20 parts of chitooligosaccharide, 5-10 parts of galactooligosaccharide, 5-10 parts of composite lactobacillus, 10-15 parts of bifidobacterium and 15-20 parts of a protective agent. The invention has the characteristics of low cost, multi-flora regulation effect and obvious regulation effect.

Description

technical field [0001] The invention relates to the technical field of prebiotic composition preparation, in particular to a preparation method, equipment and application of a prebiotic composition for efficiently regulating intestinal flora. Background technique [0002] Prebiotics refer to substances that can selectively stimulate the growth or activation of one or several types of bacteria in the intestinal tract of the host, and improve the health of the host without being digested by the host's gastrointestinal tract. Supplementing prebiotics can promote the proliferation of probiotics in the intestine and improve the structure of intestinal flora. In-depth studies have shown that a single prebiotic does not have a broad-spectrum promotion effect on probiotics. For example, xylo-oligosaccharides can promote the proliferation of Bifidobacterium adolescentis, Bifidobacterium longum and Lactobacillus salivarius, and have effects on Lactobacillus casei and Lactobacillus fer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L33/21A23L33/135B01F27/90B01F35/00B01F35/12B01F35/30B01F35/71B01F35/75B01F35/92A61K35/747A61K47/42A61K47/46A61K47/18A61K47/22A61K47/02A61P1/00A61K31/736A61K31/722A61K31/702A61K35/745
CPCY02A50/30
Inventor 皮雄娥刘伟张伟费笛波刘燕静
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES