Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Organic acid compound in purslane and extraction and separation method thereof

A separation method and compound technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, food ingredients containing natural extracts, etc., can solve the problem of low structural novelty, achieve environmentally friendly process methods, simple and fast operation methods Effect

Active Publication Date: 2022-04-19
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organic acid compound in purslane and extraction and separation method thereof
  • Organic acid compound in purslane and extraction and separation method thereof
  • Organic acid compound in purslane and extraction and separation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The invention provides an organic acid compound, the molecular formula is C 15 h 24 o 2 , named (7 E ,9 E ,12 E )-pentadecyl-7,9,12-trienoic acid, the chemical structural formulas are:

[0038] .

[0039] Described a kind of organic acid compound is named respectively according to structure (7 E ,9 E ,12 E)-pentadecyl-7,9,12-trienoic acid, Table 1 is the NMR data of this organic acid compound: 1 H-NMR with 13 C-NMR in DMSO- d 6 middle.

[0040] Table 1: Organic acid compounds of the present invention (7 E ,9 E ,12 E NMR data of )-pentadecyl-7,9,12-trienoic acid

[0041] .

[0042] A kind of organic acid compound of the present invention (7 E ,9 E ,12 E )-pentadecyl-7,9,12-trienoic acid structure identification and deduction.

[0043] (7 E ,9 E ,12 E )-pentadecyl-7,9,12-trienoic acid: yellow oil, insoluble in methanol, easily soluble in chloroform. After spotting the sample on the silica gel thin-layer plate, the spots of the test solution sp...

Embodiment 2

[0052] Example 2 Anti-inflammatory effect of the organic acid compound of the present invention.

[0053] 1 main material.

[0054] 1.1 Drugs and reagents: The organic acid compounds used in the experiment were prepared by the above method with a purity of 98%, weighed accurately, and diluted with DMSO to the required solutions for the following dosage groups. DMEM high glucose medium, fetal bovine serum (Hyclone, USA); penicillin, streptomycin (Hangzhou Sijiqing Company); LPS (Sigma, USA); IL-1 β and TNF- α ELISA kit (Cayman, USA); cell lysate (Beyontian Biotechnology Co., Ltd.).

[0055] 1.2 Cell line: RAW264.7 macrophages (US ATCC cell bank).

[0056] 1.3 Grouping: divided into LPS group and experimental group, each group.

[0057] 2 Experimental methods.

[0058] 2.1 Cell culture: DMEM high-glucose medium, add 10% fetal bovine serum, 1% antibiotics (100U / mL penicillin and 100μg / mL streptomycin), place in 37.5%, CO 2 cultured in an incubator.

[0059] 2.2 Determinati...

Embodiment 3

[0071] Example 3 The anticholinesterase effect of the organic acid compound of the present invention.

[0072] 1 main material.

[0073] 1.1 Drugs and reagents: The organic acid compounds used in the experiment were prepared by the above method with a purity of 90% to 99%, sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology ), phosphorus 5,5'-dithiobis(2-nitrobenzoic acid) (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide , ATCI, Dalian Meilun Biotechnology Co., Ltd.).

[0074] 1.2 Grouping: divided into negative control group, positive control group and experimental group, each group.

[0075] 2 Experimental methods.

[0076] 2.1 Sample preparation: Accurately weigh the sample and 1 mg of physostigmine, and use methanol as the solvent to prepare five gradients of 1 mg / mL, 0.5 mg / mL, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to an organic acid compound extracted, separated and identified from purslane and an extraction and separation method of the organic acid compound. The molecular formula of the organic acid compound is C15H24O2, and the organic acid compound is named as (7E, 9E, 12E)-pentadecyl-7, 9, 12-trienoic acid, and the structural formula of the organic acid compound is shown in the description. The invention further provides an extraction and separation method of the organic acid compound. The extraction and separation method sequentially comprises the steps of water decoction extraction, silica gel column chromatography, macroporous resin column chromatography, ODS medium-pressure column chromatography, silica gel column chromatography, Sephadex LH-20 purification and HPLC separation preparation. The structure of the compound is determined to be a novel organic acid compound by 1H-NMR, 13C-NMR and two-dimensional nuclear magnetic spectrum analysis methods. The compound has potential anti-inflammatory and anti-cholinesterase activities and the like, a preparation method is provided, and a primer and a theoretical basis are provided for developing new drugs and new components.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to an organic acid compound extracted, separated and identified from purslane medicinal material and an extraction and separation method thereof. Background technique [0002] purslane( Portulaca oleracea L.), also known as longevity vegetable and horse amaranth, is a plant of the genus Purslane in the family Portulaca oleracea. Purslane is drought and waterlogging resistant, light and shade tolerant, widely distributed and rich in resources, and has attracted much attention as a wild plant with dual uses for medicine and food. The 2020 edition of "The Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. It has the effects of clearing away heat and detoxification, cooling blood and stopping bleeding, and stopping dysentery. Insect bites, blood in the stool, hemorrhoids, metror...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/03C07C51/47A61P29/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/12
CPCC07C57/03C07C51/47A61P29/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/12A23V2002/00A23V2250/21A23V2200/30A23V2250/1882Y02A50/30
Inventor 英锡相刘佩山谭丽双李焕峰
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com