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Platycodon grandiflorum polysaccharide extraction method based on micro-thermal blasting wall surface

A technology of platycodon grandiflorum polysaccharide and extraction method, which is applied in the field of platycodon grandiflorum polysaccharide extraction based on microthermal blasting wall, can solve the problems of reducing production cost and difficulty in extracting platycodon grandiflorum polysaccharide, and achieves the effects of reducing production cost, low equipment requirements and low temperature

Active Publication Date: 2022-04-29
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting platycodon polysaccharides based on micro-heat blasting walls, which solves the problem that traditional methods are difficult to extract platycodon polysaccharides, and can improve the yield of platycodon polysaccharides when traditional hot water refluxes without destroying the polysaccharide molecular structure. while reducing production costs

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for extracting platycodon polysaccharides based on micro-heat blasting walls, the method comprising:

[0032] (1) Select the root part of fresh platycodon grandiflora, remove the skin whiskers, clean it, blow dry it, weigh it, and measure the water content, dry it at 60°C until the platycodon grandiflorum with a water content of 15% is cut longitudinally, and cut into 1~ 2cm small section;

[0033] (2) Put the bellflower segments with a water content of 15% in SO 3 Microthermal explosion reaction in gas, platycodon mass and SO 3 The volume ratio is 1g: 25mL for reaction, the treatment temperature for micro-heat explosion is 50°C, and the treatment time is 30min;

[0034] (3) will SO 3 The platycodon root after micro-heat explosion treatment is pulverized, and crosses an 80-mesh sieve;

[0035] (4) After degreasing the pulverized Platycodon grandiflorum root with petroleum ether, reflux with hot water at 60°C for 90 minutes at a material-to-liquid ratio of 1...

experiment example 1

[0038] Experimental example 1 on SO 3 SEM characterization analysis of platycodon grandiflorum treated by micro-thermal explosion technology

[0039] Using a JSM-6490LV tungsten filament scanning electron microscope (SEM) to observe untreated and treated SO 3 Surface microstructure of Platycodon grandiflorum pretreated by micro-thermal explosion technology.

[0040] Firstly, the samples before and after pretreatment are made into a block with a size of 2mm×2mm, and then the sample is adhered to the copper sample stage with conductive glue, and the sample stage is placed in a gold-spraying device for gold spraying to make the Chinese herbal medicine have Conductivity, and then can be observed, where the equipment acceleration voltage is 0.5 ~ 30kV.

[0041] From figure 2 As shown in the figure, a is the bellflower that has not been slightly heated, and b is the bellflower that has been slightly heated. It can be seen that the pretreatment process has destroyed the cell wall...

experiment example 2

[0042] Experimental Example 2 Anthrone-sulfuric acid method for measuring the absorbance value of the extract

[0043] (1) Preparation of anthrone-sulfuric acid reagent

[0044]Accurately weigh 100 mg of anthrone, and slowly add 100 mL of concentrated sulfuric acid. It should be noted that the anthrone-sulfuric acid reagent should not be stored for a long time, and should be used immediately after mixing. If the color becomes darker, it cannot be used.

[0045] (2) Preparation of glucose standard stock solution

[0046] Accurately weigh 100mg of anhydrous glucose (dried overnight in a constant temperature blast oven at 105°C in advance), put it in a beaker and add an appropriate amount of pure water to fully dissolve it, and then set the volume to a 100mL volumetric flask to obtain glucose with a concentration of 1mg / mL Standard stock solution.

[0047] (3) Drawing of glucose standard curve

[0048] Precisely pipette 1mg / mL glucose standard solution 0mL, 0.05mL, 0.1mL, 0.2m...

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Abstract

The invention discloses a platycodon grandiflorum polysaccharide extraction method based on micro-thermal explosion wall breaking, which comprises the following steps: putting platycodon grandiflorum with the water content of 10-20% into SO3 gas to carry out micro-thermal explosion reaction at the treatment temperature of 50-55 DEG C; crushing the platycodon grandiflorum subjected to SO3 micro-thermal explosion treatment, and sieving with a 20-100-mesh sieve; the method comprises the following steps: degreasing crushed platycodon roots with petroleum ether, and performing reflux extraction with hot water for 60-150 minutes at the temperature of 50-80 DEG C according to a solid-liquid ratio of 1g: (20-50) mL; and filtering the extracting solution obtained by refluxing while the extracting solution is hot, concentrating the filtrate under reduced pressure, adding absolute ethyl alcohol to precipitate polysaccharide, and centrifuging to obtain precipitate which is platycodon grandiflorum polysaccharide. According to the method, the SO3 in-situ micro-heating wall blasting technology is combined with the hot water backflow method, the extraction yield of the platycodon grandiflorum polysaccharide during traditional hot water backflow can be increased under the condition that the molecular structure of the polysaccharide is not damaged, and meanwhile the production cost is reduced.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting platycodon grandiflorum polysaccharides based on micro-heat blasting walls. Background technique [0002] Platycodon grandiflorum is a perennial herb plant, which is a traditional medicine and food homologous item. Its root tastes bitter, pungent, and slightly warm in nature. It can be eaten as a vegetable and can also be used as medicine. frequently used parts. Platycodon root contains active ingredients such as saponins, flavonoids and polysaccharides, among which polysaccharides are sugar substances with complex molecular structures formed by condensation and dehydration of multiple monosaccharide molecules. In addition to the biological effects of anti-cancer and immune regulation, polysaccharides also have anti-oxidation, hypoglycemic, blood lipid and anti-coagulant effects, and have almost no side effects on the body. [0003] Traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王淮余婉蓉姚日生罗瑜浩朱慧霞马晓静邓胜松何红波刘晴川
Owner HEFEI UNIV OF TECH
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