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Candida albicans detection powder and preparation method thereof

A Candida albicans and powder technology, which is applied in the preparation of test samples, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of low qualification rate of freeze-dried powder, long freeze-drying cycle, low freeze-drying capacity, etc. It is beneficial to long-term storage, low production cost and simple preparation method.

Pending Publication Date: 2022-05-24
湖北思欧维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, a luciferin kit coupled with recombinant chitinase appeared on the market to detect fungi. This method has improved specificity and stability, but biological enzymes are easy to inactivate and difficult to store, and chitinase detection method is widely used. Spectrum fungal detection method cannot specifically identify Candida albicans
[0003] Secondly, the existing fluorescent detection reagents are all in the form of solutions, which are prone to degradation during use and storage. At the same time, the detection reagents in the form of solutions are not suitable for long-distance transportation. The weight of solutions is heavier than that of powders, which increases transportation costs and risks.
Therefore, preparing the solution into a freeze-dried powder can effectively solve the above problems, but there are several deficiencies in the preparation of the existing freeze-dried powder of the fluorescence detection solution: the freeze-drying process is not mature enough, and in the actual large-scale freeze-drying process Among them, the qualification rate of freeze-dried powder is not high, which has an important impact on production costs; 2. The moisture in the freeze-dried powder is difficult to remove, and the structure is easy to collapse and shrink; 3. The long freeze-drying cycle makes the actual freeze-drying capacity low and energy consumption is too high Big

Method used

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  • Candida albicans detection powder and preparation method thereof
  • Candida albicans detection powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The specific raw materials are weighed according to Table 1, and the preparation steps of the remaining steps are as follows:

[0028] (1) Mix the adhesion peptide, 98% tert-butanol solution and streptavidin-fluorescein complex, and incubate at room temperature for 30 min to obtain a tert-butanol solution of the complex;

[0029] (2) after the tert-butanol solution of the obtained complex is mixed with β-cyclodextrin, dimethylacetamide, potassium dihydrogen phosphate, and glycerol, it is allowed to stand for 1 hour to remove insoluble matter to obtain a pre-freezing solution;

[0030] (3) Pre-freezing: place the pre-freezing solution in a freeze-drying cabinet, cool down to 5°C within half an hour, and maintain this temperature range for 40 minutes;

[0031] (4) Freezing and depressurization: cool down to -15°C within half an hour, and maintain this temperature range for 40min, then adjust the vacuum to 5Pa and maintain it for 2.5h;

[0032] (5) Sublimation: raise the ...

Embodiment 2

[0035] The specific raw materials are weighed according to Table 1, and the preparation steps of the remaining steps are as follows:

[0036] (1) Mix the adhesion peptide, 98% tert-butanol solution and streptavidin-fluorescein complex, and incubate for 40 min at room temperature to obtain a tert-butanol solution of the complex;

[0037] (2) after uniformly mixing the tert-butanol solution of the compound with β-cyclodextrin, dimethylacetamide, potassium dihydrogen phosphate, and glycerol, let stand for 2 hours to remove insoluble matter to obtain a pre-freezing solution;

[0038] (3) Pre-freezing: place the pre-freezing solution in a freeze-drying cabinet, cool down to -10°C within half an hour, and maintain this temperature range for 30 minutes;

[0039] (4) Freezing and depressurization: cool down to -25°C within half an hour, and maintain this temperature range for 30min, then adjust the vacuum to 10Pa and maintain it for 2h;

[0040] (5) Sublimation: raise the temperature...

Embodiment 3

[0043] The specific raw materials are weighed according to Table 1, and the preparation steps of the remaining steps are as follows:

[0044] (1) Mix the adhesion peptide, 98% tert-butanol solution and streptavidin-fluorescein complex, and incubate for 40 min at room temperature to obtain a tert-butanol solution of the complex;

[0045] (2) after uniformly mixing the tert-butanol solution of the compound with β-cyclodextrin, dimethylacetamide, potassium dihydrogen phosphate, and glycerol, let stand for 2 hours to remove insoluble matter to obtain a pre-freezing solution;

[0046] (3) Pre-freezing: place the pre-freezing solution in a freeze-drying cabinet, cool down to -10°C within half an hour, and maintain this temperature range for 40 minutes;

[0047] (4) Freezing and depressurization: cool down to -25°C within half an hour, and maintain this temperature range for 40min, then adjust the vacuum to 10Pa and maintain it for 2.5h;

[0048] (5) Sublimation: raise the temperatu...

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Abstract

The invention relates to the technical field of detection preparations, in particular to candida albicans detection powder and a preparation method thereof. The detection powder is prepared from the following raw materials: adhesive peptide, a streptavidin fluorescein compound, a freeze-drying protective agent, a C2-C4 alcoholic solution, a penetrant, a stabilizer and an anti-quenching agent, and the concentration of the C2-C4 alcoholic solution is 95%-98%. The tert-butyl alcohol solution is used as an integral solution, and the tert-butyl alcohol is relatively stable in property, so that the influence on the use effect of the fluorescent reagent due to the generation of impurities in the freeze-drying process is avoided; secondly, the water content in the product is low, and long-term storage and preservation are facilitated.

Description

technical field [0001] The invention relates to the technical field of detection preparations, in particular to a Candida albicans detection powder and a preparation method thereof. Background technique [0002] Fluorescence detection is a rapid detection method for fungi that has been widely used. The fluorescent detection fungus kits circulating in the market are mainly composed of fluorescent reagents, solubilizers and background dyes. The commonly used fluorescent reagent is CalfluorWhite (CFW), which can combine with the chitin component in the fungal cell wall and generate blue fluorescence under the excitation of ultraviolet light. However, CFW has poor specific binding ability and can bind to most fungi. It needs to manually determine the genus of bacteria. At the same time, the quenching time is short, which can make the background tissue emit bright fluorescence, which affects the judgment of fungi and background. Therefore, CFW generally needs to be combined wit...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/42
CPCG01N21/6428G01N1/42G01N2021/6432Y02A50/30
Inventor 陈强
Owner 湖北思欧维生物科技有限公司