Preparation method of fluorescent polystyrene microspheres

Abstract

The invention discloses a preparation method of fluorescent polystyrene microspheres, which comprises the following steps: firstly, combining fluorescent dye molecules coupled with azide with polystyrene microspheres through a swelling method; and bonding fluorescent dye molecules to the polystyrene microspheres by using an intermediate generated by pyrolyzing the azide at a high temperature to obtain the fluorescent polystyrene microspheres. A large number of carboxyl and hydroxyl functional groups are uniformly distributed inside and outside the prepared fluorescent coding microspheres, the functional groups not only enable the coding microspheres to be not easy to agglomerate and be slightly influenced by the environment, but also can effectively reduce non-specific adsorption of the microspheres to proteins due to the fact that the surfaces of the microspheres have a large number of hydroxyl functional groups; and under the condition of adding the same fluorescent dye concentration, the fluorescence intensity of the fluorescent encoding microspheres prepared by the invention is far higher than that of fluorescent polystyrene microspheres prepared by the existing organic swelling method, and the fluorescent performance is stable.

Description

technical field [0001] The invention relates to a preparation method of fluorescent polystyrene microspheres. Background technique [0002] Fluorescent polystyrene microspheres have a wide range of applications in the field of biomedicine, and provide important technical support for disease diagnosis, food testing, and water environment testing. In the existing methods for preparing fluorescent polystyrene microspheres based on fluorescent organic dyes, such as adsorption method and chemical bonding method, the fluorescence stability of fluorescent polystyrene microspheres prepared by these surface modification methods is not ideal, such as CN110484234A, Patents such as CN109294554A. In the entrapment method and copolymerization process of dye molecules, a key problem of dye molecules interfering with the polymerization process is the wide size distribution of dyes, dye quenching, and pair coding (coding refers to binding different content of dyes, thus having different flu...

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Problems solved by technology

In the existing methods for preparing fluorescent polystyrene microspheres based on fluorescent organic dyes, such as adsorption and chemical bonding, the fluorescence stability of fluorescent polystyrene microspheres prepared by these surface modification methods is not ideal, such as CN110484234A, CN109294554A and other patents

In the embedding method and copolymerization process of dye molecules, a key problem of dye molecules interfering with the polymerization process is wide dye size distribution, dye quenching, and pair coding (coding refers to the combination of dyes with different content, so as to have different fluorescent signals) Accuracy produces side effects, such as CN111875744A, CN111057174A, CN109943317A and other patents

In addition, host-guest structural systems have been proposed to assemble dye-doped silica and microsphere supports, but the complexity of preparation limits its application.

The organic dye swelling method is one of the most attractive platforms for preparing fluorescently encoded microspheres due to its simple operation, good stability, and accurate quantitative dyeing, such as CN111019643B, CN108359059B, CN108047382B and other patents, but the fluorescent polymer microspheres prepared by the swelling method Relying only on physical binding of fluorescent dyes, there is a problem of easy leakage

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