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Drug-loaded metal organic framework composite electrostatic spinning fiber membrane as well as preparation method and application thereof

A metal-organic framework, electrospinning technology, applied in rayon manufacturing, fiber chemical characteristics, conjugated synthetic polymer rayon, etc. Beneficial to cell growth, good biocompatibility, prolonged release period

Pending Publication Date: 2022-08-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dressing does not have antibacterial properties and cannot meet the various requirements for wound dressings in the complex environment of the wound

Method used

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  • Drug-loaded metal organic framework composite electrostatic spinning fiber membrane as well as preparation method and application thereof
  • Drug-loaded metal organic framework composite electrostatic spinning fiber membrane as well as preparation method and application thereof
  • Drug-loaded metal organic framework composite electrostatic spinning fiber membrane as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of drug-loaded metal-organic framework composite electrospinning fiber membrane

[0044] (1) Preparation of ZIF-8 nanoparticles: take 400mg of zinc nitrate hexahydrate in 10mL of anhydrous methanol, take 1g of 2-methylimidazole in 15mL of anhydrous methanol, ultrasonically accelerate the dissolution, mix the 2-methylimidazole The solution was loaded with a syringe, added to anhydrous methanol solution of zinc nitrate hexahydrate at a pushing speed of 0.3 mL / min under the control of a micro-syringe pump, magnetically stirred for 12 h, centrifuged at 10,000 rpm and washed twice with anhydrous methanol. , and vacuum dried at 40 °C for 24 h to obtain ZIF-8 nanoparticles.

[0045] Preparation of DMOG-loaded ZIF-8 nanoparticles: take 400 mg of zinc nitrate hexahydrate in 10 mL of anhydrous methanol, take 0.2 g of DMOG and 1 g of 2-methylimidazole in 15 mL of anhydrous methanol, sonicate to accelerate the dissolution, mix DMOG and 2 -The mixed solution o...

Embodiment 2

[0048] Example 2 Scanning electron microscope observation of drug-loaded metal-organic framework composite electrospinning fiber membrane

[0049] ZIF-8 and DMOG@ZIF-8 were prepared according to the method of Example 1. ZIF-8 and DMOG@ZIF-8 were taken in anhydrous ethanol, dispersed by ultrasonic, and then added dropwise onto a titanium sheet. After being sprayed with gold for 60 s, the morphology and particle size of the nanoparticles were observed under a high-magnification scanning electron microscope. like Figure 1a , Figure 1b As shown, the particle size distributions of ZIF-8 and DMOG@ZIF-8 are between 70-120 nm and 90-150 nm, respectively.

[0050] The drug-loaded metal-organic framework composite electrospinning fiber membrane was prepared according to the method of Example 1. The fiber film sample was attached to the conductive adhesive, and then sprayed with gold for 60 s with a gold sprayer and observed with a tungsten filament scanning electron microscope. lik...

Embodiment 3

[0051] Example 3 Characterization of antibacterial properties of drug-loaded metal-organic framework composite electrospinning fiber membrane

[0052] Drug-loaded metal-organic framework composite electrospinning fiber membranes were prepared according to the method of Example 1, and fiber membranes with different DMOG@ZIF-8 addition amounts of 20 mg were cut into 1 mL of PBS, and diluted Escherichia coli or gold was added to them. Staphylococcus aureus, and make the final concentration of bacteria 10 5 CFU / mL, after the material and bacteria were co-cultured for 4 hours, the culture medium was uniformly diluted to an appropriate multiple by ultrasonic vibration, 50 mL of liquid was taken and plated on solid agar medium, and the coated agar medium was plated at 37 °C. Incubate overnight and observe the number of colonies on the plate. When the amount of DMOG@ZIF-8 added is ≥1mg / mL, the composite fiber membrane has obvious antibacterial effect on Escherichia coli (see Figure...

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Abstract

The invention discloses a drug-loaded metal organic framework composite electrostatic spinning fiber membrane as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) adding ZIF-8 nanoparticles loaded with dimethyl oxalyl glycine into hexafluoroisopropanol, adding gelatin and polycaprolactone after ultrasonic dispersion, and fully stirring to obtain an electrospinning solution; and (2) filling the electrospinning liquid in the step (1) into an injector, and preparing the drug-loaded metal organic framework composite electrostatic spinning fiber membrane by an electrostatic spinning method. In the invention, ZIF-8 not only serves as an antibacterial agent to exert the antibacterial property, but also serves as a carrier of DMOG to regulate and control the release rate of DMOG together with the Gel-PCl fiber membrane. In addition, the composite fiber membrane also has good water vapor permeability, can simulate an extracellular matrix, has good antibacterial property and biocompatibility, can promote migration of endothelial cells, in-vitro blood vessel formation and other properties, and has good application prospects in the field of chronic refractory wound repair.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a drug-loaded metal-organic framework composite electrospinning fiber membrane and a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body and plays an important role in regulating human metabolism and resisting external aggression. However, various pathological or accidental accidents can easily cause skin wounds, and when the wound is blocked by bacterial infection and other factors, normal healing , it is easy to turn into a refractory wound, which brings serious economic losses to the human society. Bacterial infection and insufficient new blood vessels are the two main obstacles to the healing of tissue refractory wounds. [0003] Wound dressings are the most economical and practical means of wound treatment, and have a long history of application. However, with the continuous development of human researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/728D04H1/435D04H1/4382D01F1/10D01F8/02D01F8/14
CPCD04H1/728D04H1/435D04H1/43825D01F8/02D01F8/14D01F1/103D10B2401/13D10B2509/022
Inventor 方立明尹磊汤绮雯柯琦赖姗姗范鹏辉苏健裕
Owner SOUTH CHINA UNIV OF TECH
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