Microbial drug material, its preparing method and usage
A production method and a technology of raw materials, applied in the direction of antineoplastic drugs, pharmaceutical formulations, medical raw materials derived from fungi, etc., can solve the problems of bioavailability to be improved, and achieve good clinical application effects, safe use, and good synergy Effect
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Embodiment 1
[0045] 1. Preparation of Bioactive Selenium
[0046] Add 0.3g of yeast extract, 5g of sodium acetate, 1g of ammonium oxide, 1g of potassium dihydrogen phosphate, 0.5g of magnesium oxide, and 3g of sodium bicarbonate into 1000ml of distilled water, stir and dissolve, boil for sterilization, and reduce the humidity to 32°C and press 5wt% The proportion of Rhodopseudomonas (Rhodopseudomonas), especially Rhodopseudomonas sanguinus (Rhodopseudomonas palustris, Rps.Rutila), cultured in a solar wet room at 25°C-26°C for 3 days, then added 200mg / kg concentration of sodium selenite continued to cultivate for 5 days, and the material gradually turned dark red, indicating that it had matured. Sampling and testing showed that the number of live bacteria was 1.0-1.1 billion / ml.
[0047] 2. Preparation of Fungal Polysaccharides
[0048] Select 1000g of the fresh fruiting body of the natural edible fungus Flammulina velutipes, add edible alcohol according to 20 times the amount, extract a...
Embodiment 2
[0053] 1. Preparation of Bioactive Selenium
[0054] Add 1g of yeast extract (brewer's yeast Brewer'Sreast), 3g of sodium acetate, 5g of ammonium oxide, 3g of potassium dihydrogen phosphate, 0.1g of magnesium oxide, and 1g of sodium bicarbonate into 1000ml of distilled water, stir to dissolve, boil to sterilize, and dehumidify After reaching 32 DEG C, insert Rhodospirilium bacterial classification in the ratio of 10wt%, bacterial classification is 0192 (Rhodospirilium1 altogether 1 strains), put solar greenhouse 29 DEG C-30 DEG C after cultivating for 2 days, add the selenous acid of 500mg / kg concentration For cobalt, continue to cultivate for 3 days, and the material gradually turns dark red, indicating that it has matured. Sampling and detection, the number of viable bacteria can generally reach 1.9-2 billion / ml.
[0055] 2. Preparation of Fungal Polysaccharides
[0056] Select 1000g of fresh fruiting body of Flammulina velutipes, 10 times the amount of selected natural ed...
Embodiment 3
[0060] 1. Preparation of Bioactive Selenium
[0061] Add 0.1g of yeast extract, 1g of sodium acetate, 0.1g of ammonium oxide, 5g of potassium dihydrogen phosphate, 3g of magnesium oxide, and 10g of sodium bicarbonate into 1000ml of distilled water, stir and dissolve, boil for sterilization, and reduce the humidity to 32°C and press 8wt% The proportion of Rhodopseudomonas strains (Rhodopseudomonas globosa Rps.Globiformis) was inserted, and after cultivating for 2.5 days in a solar wet room at 28°C-29°C, calcium selenite at a concentration of 500mg / kg was added, and the cultivation was continued for 3 Days, the material gradually turns dark red, indicating that it has matured. Sampling and detection, the number of viable bacteria can generally reach 1.9-2 billion / ml.
[0062] 2. Preparation of Fungal Polysaccharides
[0063] Select 1000g of fresh fruiting body of Flammulina velutipes, add 15 times the amount of edible alcohol, leaching at room temperature (30°C) until pectin i...
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