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Biological degradable macromolecule microspheres of carrying insulin and preparation method

A polymer microsphere, biodegradable technology, applied in the field of biodegradable polymer microspheres and preparation, to achieve the effects of sustained release, improved insulin release behavior, and uniform distribution

Inactive Publication Date: 2005-01-12
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, this polymer microsphere has the advantages of insulin liposomes and poly-α-cyanoacrylate insulin nanocapsules, protects insulin from acid, alkali and protease in the digestive system, and improves the bioavailability of insulin. And its stability is much better than that of liposomes. It uses biodegradable polymers as encapsulation materials, which will overcome the shortcomings of poly-α-cyanoacrylates that cannot be biodegraded and accumulate in the human body.

Method used

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  • Biological degradable macromolecule microspheres of carrying insulin and preparation method
  • Biological degradable macromolecule microspheres of carrying insulin and preparation method
  • Biological degradable macromolecule microspheres of carrying insulin and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of Insulin Nanoparticles

[0047] Add 10 drops of Tween 80 and 20 mg of insulin into 100 ml of double distilled water with pH=2.6, and stir to dissolve it. Under stirring, a dilute solution of sodium hydroxide is added dropwise to the solution to neutralize the solution to neutrality, so as to obtain a nano particle solution of insulin. Insulin nanoparticles were obtained by centrifugation at 20,000 rpm and freeze-dried. The particle diameter of the obtained insulin nanoparticle is 50~200nm, and its transmission electron microscope photograph sees figure 1 . It can be seen that the obtained nanoparticles are all preferably spherical, and their particle diameters are in the range of 50-200nm.

Embodiment 2

[0049] Polylactide-glycolide copolymer with a molecular weight of 50,000, which contains 80% lactide and 20% glycolide, 50mg is dissolved in 5ml dichloromethane, and polylactide with a diblock molecular weight of 30,000 and 5000 respectively Dissolve 50 mg of ester-polyethylene glycol diblock copolymer (PLA-PEG) in 5 ml of dichloromethane. Blend the two solutions together, add 4 drops of Span 80, and stir well. Add 10 mg of insulin nanoparticles into the polymer solution, and emulsify with high-speed stirring for 60 seconds at 10,000 rpm to obtain a suspoemulsion. The above suspoemulsion was added to 100 ml of aqueous solution containing 2% polyvinyl alcohol, and stirred at a high speed for 90 seconds at 8000 rpm to obtain a solid powder emulsion in oil-in-water. Stir at room temperature for 4 hours to volatilize the solvent, then centrifuge at high speed to separate insulin-coated microspheres, wash with double distilled water, freeze-dry the obtained microspheres, and obtai...

Embodiment 3

[0054] Polylactide-glycolide copolymer with a molecular weight of 20,000, which contains 80% lactide and 20% glycolide, 100 mg is dissolved in 5 ml of dichloromethane, and polyhexene with a diblock molecular weight of 50,000 and 2000 is respectively 50 mg of lactone-polyethylene glycol diblock copolymer (PCL-PEG) was dissolved in 5 ml of dichloromethane. Blend the two solutions together, add 5 drops of Span 80, and stir well. Add 15 mg of insulin nanoparticles into the polymer solution, and emulsify with high-speed stirring for 60 seconds at 10,000 rpm to obtain a suspoemulsion. The above-mentioned suspoemulsion was added to 100 ml of an aqueous solution containing 5% gelatin, and stirred at a high speed for 90 seconds at 8000 rpm to obtain a solid powder emulsion in oil-in-water. Stir at room temperature for 4 hours to evaporate the solvent, then centrifuge at a high speed to separate insulin-coated microspheres, wash with double distilled water, freeze-dry, and obtain insul...

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Abstract

A diodegradable high-molecular microsphere carrying insulin is prepared through preparing the nanoparticles of insulin, preparing the mixed solution of different two polymers, and dual-layer coating of said insulin nanoparticles.

Description

technical field [0001] The invention relates to a biodegradable polymer microsphere loaded with insulin and a preparation method thereof. Background technique [0002] Diabetes is a worldwide disease, mainly manifested as glucose metabolism disorder caused by endocrine disorders. The incidence of diabetes is increasing year by year, and it has become the third leading cause of death after cardiovascular disease and cancer. [0003] There are two types of diabetes, one is insulin-dependent diabetes mellitus (IDDM), also known as type 1 diabetes, which mostly appears in children and young adults and can be attributed to a genetic factor or an abnormal immune mechanism As a result, almost no insulin is produced in the body. The other, non-insulin-dependent diabetes mellitus (NIDDM), also known as type 2 diabetes, is diabetes that often begins in middle age. The patient can secrete insulin, but its function cannot be performed normally. [0004] Insulin is the drug of choice...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K38/28A61K47/34A61P3/10
Inventor 景遐斌张雪飞陈学思徐效义胡俊丽
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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