Biological degradable macromolecule microspheres of carrying insulin and preparation method
A polymer microsphere, biodegradable technology, applied in the field of biodegradable polymer microspheres and preparation, to achieve the effects of sustained release, improved insulin release behavior, and uniform distribution
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Examples
Embodiment 1
[0046] Example 1: Preparation of Insulin Nanoparticles
[0047] Add 10 drops of Tween 80 and 20 mg of insulin into 100 ml of double distilled water with pH=2.6, and stir to dissolve it. Under stirring, a dilute solution of sodium hydroxide is added dropwise to the solution to neutralize the solution to neutrality, so as to obtain a nano particle solution of insulin. Insulin nanoparticles were obtained by centrifugation at 20,000 rpm and freeze-dried. The particle diameter of the obtained insulin nanoparticle is 50~200nm, and its transmission electron microscope photograph sees figure 1 . It can be seen that the obtained nanoparticles are all preferably spherical, and their particle diameters are in the range of 50-200nm.
Embodiment 2
[0049] Polylactide-glycolide copolymer with a molecular weight of 50,000, which contains 80% lactide and 20% glycolide, 50mg is dissolved in 5ml dichloromethane, and polylactide with a diblock molecular weight of 30,000 and 5000 respectively Dissolve 50 mg of ester-polyethylene glycol diblock copolymer (PLA-PEG) in 5 ml of dichloromethane. Blend the two solutions together, add 4 drops of Span 80, and stir well. Add 10 mg of insulin nanoparticles into the polymer solution, and emulsify with high-speed stirring for 60 seconds at 10,000 rpm to obtain a suspoemulsion. The above suspoemulsion was added to 100 ml of aqueous solution containing 2% polyvinyl alcohol, and stirred at a high speed for 90 seconds at 8000 rpm to obtain a solid powder emulsion in oil-in-water. Stir at room temperature for 4 hours to volatilize the solvent, then centrifuge at high speed to separate insulin-coated microspheres, wash with double distilled water, freeze-dry the obtained microspheres, and obtai...
Embodiment 3
[0054] Polylactide-glycolide copolymer with a molecular weight of 20,000, which contains 80% lactide and 20% glycolide, 100 mg is dissolved in 5 ml of dichloromethane, and polyhexene with a diblock molecular weight of 50,000 and 2000 is respectively 50 mg of lactone-polyethylene glycol diblock copolymer (PCL-PEG) was dissolved in 5 ml of dichloromethane. Blend the two solutions together, add 5 drops of Span 80, and stir well. Add 15 mg of insulin nanoparticles into the polymer solution, and emulsify with high-speed stirring for 60 seconds at 10,000 rpm to obtain a suspoemulsion. The above-mentioned suspoemulsion was added to 100 ml of an aqueous solution containing 5% gelatin, and stirred at a high speed for 90 seconds at 8000 rpm to obtain a solid powder emulsion in oil-in-water. Stir at room temperature for 4 hours to evaporate the solvent, then centrifuge at a high speed to separate insulin-coated microspheres, wash with double distilled water, freeze-dry, and obtain insul...
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Abstract
Description
Claims
Application Information
- IPC
- A61K9/14; A61K38/28; A61K47/34; A61P3/10
- Inventors
- 景遐斌; 张雪飞