Surface functionalization of gold or silver nanoparticle, and colorimetry detection method for molecule by using the same

A silver nanoparticle and surface functionalization technology, which is applied in biological testing, color/spectral characteristic measurement, material inspection products, etc., can solve the problems of difficult realization of gold nanoparticle labeling, achieve easy purification and quantitative analysis, and avoid detection equipment , the effect of chemical stability

Inactive Publication Date: 2006-07-19
SOUTHEAST UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thilylated oligonucleotides are commercially available, but most other molecules that are thiolated are not commercially available, and therefore, labeling o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Surface functionalization of gold or silver nanoparticle, and colorimetry detection method for molecule by using the same
  • Surface functionalization of gold or silver nanoparticle, and colorimetry detection method for molecule by using the same
  • Surface functionalization of gold or silver nanoparticle, and colorimetry detection method for molecule by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Determination of DNA by gold nanoparticle-labeled oligonucleotide probes

[0015] Coupling of oligonucleotides to dextran: 0.16ml, 25mg / ml 4-nicotinic acid succinimide ester hydrazine acetone hydrazone (C6-succinimidyl 4-hydrazinonicotinate acetone hydrazone) and 25mg / ml N-succinyl Amine-3-(2-pyridyldithio)-propionate (SPDP, N-succinimidyl3-(2-pyridyldithio)propionate) in anhydrous DMSO solution was added to 4ml, 2mg / ml aminodextran (MW 70KDa ) in 1×PBS solution at room temperature for 2 hours. The resulting dextran conjugate was purified on a Sephadex G25 column using 0.1M NaAc (pH=4.5) as the eluent. The amount of dithio and hydrazone functional groups generated can be quantified with 1,4-dithiothreitol and 4-nitrobenzaldehyde, respectively. Excess aldehyde-modified oligonucleotides were added to a 0.1M NaAc (pH4.5) solution of dextran conjugates and reacted for 12 hours, and the unreacted oligonucleotides were separated and removed with a 30KDa molecular...

Embodiment 2

[0018] Example 2: Silver nanoparticle-labeled oligonucleotide probes measure DNA

[0019]Coupling of oligonucleotides to dextran: 0.16ml, 25mg / ml 4-nicotinic acid succinimide ester hydrazine acetone hydrazone (C6-succinimidyl 4-hydrazinonicotinate acetone hydrazone) and 25mg / ml N-succinyl Amine-3-(2-pyridyldithio)-propionate (SPDP, N-succinimidyl3-(2-pyridyldithio)propionate) in anhydrous DMSO solution was added to 4ml, 2mg / ml aminodextran (MW 70KDa ) in 1×PBS solution at room temperature for 2 hours. The resulting dextran conjugate was purified on a Sephadex G25 column using 0.1M NaAc (pH=4.5) as the eluent. The amount of dithio and hydrazone functional groups generated can be quantified with 1,4-dithiothreitol and 4-nitrobenzaldehyde, respectively. Excessive CHO-modified oligonucleotide probes were added to 0.1M NaAc (pH4.5) solution of dextran conjugates and reacted for 12h, then separated and removed unreacted oligonucleotide probes with a 30KDa molecular weight cut-off ...

Embodiment 3

[0022] Example 3: Gold Nanoparticles Label Biotin Molecules

[0023] Conjugation of biotin to dextran: 5 mg N-succinimide-3-(2-pyridyldithio)-propionate (SPDP) dissolved in 100 μl, 50 mg / ml biotinaminocaproic acid-N- Hydroxysuccinimide ester (Biotinamidohexanic acid N-hydroxysuccinimide ester) anhydrous DMSO solution, this solution was added dropwise to 2.5ml, 2mg / ml aminodextran (MW 70KDa) NaHCO 3 (50mM) solution at room temperature for 2 hours. The resulting dextran conjugates are purified by dialysis in aqueous solution for further use. The amount of biotin on the dextran conjugate can be quantified with HABA (4'-hydroxyazobenzene-2-carboxylic acid).

[0024] Preparation of gold nanoparticle-labeled biotin-dextran conjugate probe: add different amounts of biotin-dextran conjugate to a certain amount of gold nanoparticle solution, and record the solution after filtering through a 0.20 μm membrane According to the UV absorption of gold nanoparticles at 520nm, the desired a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for labeling and colorimetric detecting the biology molecule by surface fictionalized gold or silver nanometer particle. The probe molecule for identifying the biology molecule and the nail molecule for connecting the gold or silver nanometer particle first through the gluglucosan whose double function agent is covalence reformatted macromolecule to form the deformation coated the gold or silver nanometer particle, it uses the self-component on the surface of the gold or silver nanometer particle by the nail group of the deformation to form gluglucosan molecule coated gold or silver nanometer particle after purifying and quantifying the deformation.

Description

technical field [0001] The invention relates to a method for colorimetrically detecting biomolecules using surface-functionalized gold or silver nanoparticles as markers, and belongs to the technical field of nano-labeling and detection of biomolecules. Background technique [0002] Label analysis is one of the most important methods for the detection of biomolecules (nucleic acid, protein, etc.). Due to the weak nature of many biomolecules that can be analyzed by themselves, in order to obtain high-sensitivity detection, it is often analyzed with the help of external markers to obtain measurable signals. Commonly used labels can be divided into luminescent labels, enzyme labels, electrochemical labels and so on. Among them, the luminescent labeling method is the most important method, such as various fluorescent labels and chemiluminescent labels. The sensitivity of luminescent label detection depends largely on the luminescent intensity of the luminescent label. [0003...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/52G01N21/25
Inventor 陈扬
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap