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Method for predicting calcium antagonist medicine effect and application thereof

A calcium antagonist and compound technology, applied in the field of medicine, can solve the problems such as inability to tailor-made, economic losses of patients, inability to improve the efficacy of drugs, toxic and side effects, etc.

Inactive Publication Date: 2011-10-26
深圳市奥萨药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] Due to individual differences in drug reactions, doctors cannot choose drugs and drug compatibility according to the individual's needs when choosing drugs, and cannot improve the efficacy of drugs and reduce the occurrence of toxic and side effects, which may delay the timing of treatment and cause economic losses to patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Determining the Glu429Ala(AC) polymorphism site of the MTHFR gene and predicting the antihypertensive effect of the calcium antagonist class antihypertensive drug nifedipine

[0059] (1) Determination of the genotype of the Glu429Ala(AC) polymorphism site of the MTHFR gene:

[0060] (1) Extract the genomic DNA of the host cell:

[0061] (a) Add 30ml of erythrocyte lysate to the whole blood, shake slowly, and let stand at room temperature for 10 minutes. During this period, shake several times to completely lyse the erythrocytes;

[0062] (b) Centrifuge at 4°C at 2000 rpm for 10 minutes, remove the supernatant, break up the precipitated leukocytes on a rotary shaker, add 40ul of protease and 50ul of RNase, shake well, add 15ml of leukocyte lysate, Mix well in a 37°C water bath for 20 minutes, take it out, and put it in cold water;

[0063] (c) Add 4ml of cold protein precipitation solution, mix well and place in -20°C refrigerator for 5 minutes, take it out ...

Embodiment 2

[0094] Example 2: Determination of the Ala222Val polymorphism site of the MTHFR gene and prediction of the antihypertensive effect of the calcium antagonist antihypertensive drug nifedipine

[0095] (1) Determination of the genotype of the Ala222Val polymorphism site of the MTHFR gene:

[0096] (1) On the standard operating method and operating procedures, adopt the same conventional method as described in Example 1 to extract the genomic DNA of the host cell

[0097] (2) Detection of the MTHFRAla222Val polymorphic site by PCR and restriction enzyme fragment length polymorphism analysis method (PCR-RFLP):

[0098] PCR-specific primers were designed according to the MTHFR Ala222Val gene sequence, including PCR forward primers and PCR reverse primers, and conventional PCR amplification was performed according to the following conditions.

[0099] Primer sequence:

[0100] Sense: 5'-CAAAGG CCA CCC CGAAGC-3'

[0101] Antisense: 5'-AGG ACG GTG CGG TGA GAG TG-3'

[0102] PCR rea...

Embodiment 3

[0120] Example 3: Determination of the MTRR His595Tyr (CT) polymorphism site and prediction of the antihypertensive effect of the calcium antagonist class antihypertensive drug nifedipine

[0121] (1) Determination of the genotype of the His595Tyr (CT) polymorphic site of the MTRR gene:

[0122] (1) Extract the genomic DNA of the host cell:

[0123] On the basis of standard operating procedures, the genomic DNA in salivary cells was extracted by salt precipitation. Store DNA at -20°C.

[0124] (2) The genotype of MTRR His595Tyr (C1894T) polymorphism was detected by TaqMan method.

[0125] (a) Amplify the MTRR His595Tyr gene polymorphic site and its flanking sequences with a PCR instrument, containing 10ng of genomic DNA in a 5ul PCR reaction system, and PCR Master Mix NoAmpErase UNG of 2.5ul TaqMan 2X Universal (composition includes: AmpliTaq Gold DNA Polymerase, dNTPs with Dutp, Passive Reference, optimized buffer), and 0.72uM forward primer, 0.72uM reverse primer and two ...

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PUM

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Abstract

The invention relates the method for forecasting the drug-effect. The method measures the polymorphism gene type to forecast the drug-effect of calcium antagonist drug. The method forecasts the drug-effect of calcium antagonist drug by measuring the polymorphism gene type of key enzyme gene on the aminothiopropionic acid metabolism path.; at the mean time, according to the effect, prepare the compound drug which can improve the drug-effect of calcium antagonist by adjusting the preparation of aminothiopropionic acid metabolism path. The invention improves the effective rate of clinical drug therapeutics and the safety of clinical drug, and reduces the drug toxin side effect and cost.

Description

technical field [0001] The invention relates to a method for predicting drug efficacy and its application, in particular to a method for predicting the drug effect of calcium antagonist drugs by measuring the genotype of gene polymorphism sites and its application, which belongs to the field of medicine. Background technique [0002] Hypertension is one of the most common chronic diseases in my country and the world. Recent research results show that the prevalence of hypertension among residents over the age of 18 in my country is 18.8%, and the number of patients nationwide is estimated to be 160 million. Hypertension is the main cause of life-threatening heart and cerebrovascular diseases such as myocardial infarction, stroke, and renal insufficiency. Blood pressure level is linearly correlated with the incidence of cardiovascular disease. Epidemiological studies have shown that elevated blood pressure is an independent risk factor for stroke and coronary heart disease....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A61K31/4422A61K31/519A61K31/714
Inventor 蒋善群洪秀梅文育峰王燕毛广运唐根富张善春余运贤王滨燕邢厚恂戴成祥陈光亮徐希平
Owner 深圳市奥萨药业有限公司
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