ELISA kit for detecting clenbuterol and detection method thereof, and animal tissue sample preparing method before detection

An enzyme-linked immunosorbent reagent and substrate technology, which is used in measurement devices, special data processing applications, color/spectral property measurement, etc. High requirements are required to achieve the effect of low cost, easy separation and improved sensitivity
CN1885038AActive Publication Date: 2006-12-27SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2006-12-27

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Abstract

The invention relates to an enzyme couple immune detecting field, especially disclosing a clenobuterol enzyme immune agent box, relative detecting method, and a method for preparing the sample of animal organism, wherein said box comprises a box body, a 96 / 40 porous enzyme mark plate, a horse peroxide enzyme mark antibody, a clenobuterol standard solution, a substrate liquid, a substrate buffer liquid and a reaction ending liquid; the holes of said mark plate packs the packing antibody that combined with the clenobuterol antibody. The invention uses direct competitive enzyme couple immune adsorption analysis technique, to simplify the operation and reaction time, to reduce error. The inventive method has simple operation, low cost, and short time, while the extracted sample can be directly used in enzyme couple immune method and golden mark immune laminated analysis, to be sample of prior analysis.
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Description

technical field

[0001] The invention relates to the field of enzyme-linked immunoassay detection, in particular to a clenbuterol detection ELISA kit, a detection method thereof, and a sample preparation method of animal tissue before detection. Background technique

[0002] Clenbuterol, commonly known as clenbuterol, belongs to beta stimulant, chemical name is 2-[(tert-butylamino)methyl]-4-ammonia-3,5-dichlorobenzyl alcohol hydrochloride, medicine It is called "Cetansu", which has been illegally added to the feed in recent years to increase the lean meat rate of fat animals and accelerate the growth of animals. Because the additive dose is 5 to 10 times the therapeutic dose, it has high residues in the animal body and is given to the animal. harm to consumers. Since 1997, my country's Ministry of Agriculture and other relevant departments have issued documents many times, listing clenbuterol as a prohibited drug that seriously endangers people's health, and prohibiting its ...

Claims

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