The invention relates to a novel amido-linkage imidazolium salt gemini surfactant and a preparation method thereof, and the preparation method can be used for solving the problems that the existing preparation method of the amido-linkage heterocyclic gemini surfactant is complicated to operate, high in cost and reaction temperature, long in reaction time, and large in solvent toxicity. The preparation method comprises the following steps of: I, N-alkylation; II, amidation; III, quaternization to obtain the product amido-linkage imidazolium salt gemini surfactant. The preparation method is simple for three-step reaction operations, capable of shortening the reaction time, and mild in reaction conditions, the solvent toxicity is small, the raw material cost is low, the hydrophilic property of the novel amido-linkage imidazolium salt gemini surfactant can be greatly improved through introducing strong-polarity amido linkage and two positive nitrogen ion heads, the novel amido-linkage imidazolium salt gemini surfactant has good biodegradability through introducing nitrogen heterocyclic ring and amido linkage, and the environmentally-friendly gemini surfactant can be used in multiple fields of cleaning, sterilization, restraint of metal corrosion, sewage treatment and the like.