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Process for the preparation of protease inhibitor using novel alkalothermophilic Bacillus sp

a technology of alkalothermophilic bacteria and protease inhibitor, which is applied in the field of new strains of alkalothermophilic bacteria, can solve the problems of harmful cells, no report is available for the preparation of protease inhibitors, and excessive pepsin secretion has harmful effects on the stomach

Inactive Publication Date: 2005-06-30
DASH CHANDRAVANU +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides a process for making a low molecular weight peptidic aspartic acid protease inhibitor using a novel alkalothermophilic strain of Bacillus sp. This strain has been deposited at the ATCC and has Accession number PTA 972. The invention also provides an anti-fungal agent using this low molecular weight aspartic acid protease inhibitor."

Problems solved by technology

Proteolytic enzymes outside of microbial cells hydrolyze organic nitrogen compounds in the medium, so they are thought to be harmful to cells.
So far no report is available for the preparation of the protease inhibitor using alkalothermophilic Bacillus sp.
Excess secretion of pepsin has harmful effects on the stomach as it damages the digestive tract and causes stomach ulcer or duodenal ulcer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Eighteen hour grown AT Bacillus NCIM 59 on an agar slant, at 50° C., was inoculated into a medium containing glucose-1 %, peptone-0.75%, beef extract-0.75%, sodium chloride-0.3%, dipotassium hydrogen phosphate-0. 1%, magnesium sulfate-0.1% and soyameal-2.0%, and this fermentation medium was incubated on a rotary shaker at 50° C. for 12 hours 10% v / v of the freshly grown inoculum was added to the fermentation flasks. After 48 hours of growth on a rotary shaker at 50° C. the cells and the residual soyameal was removed by centrifugation. To 100 ml of centrifuged supernatant 10 grams of activated charcoal was added and incubated overnight at 4° C. This charcoal treated broth was filtered through filter paper 2-3 times to ensure there was no trace of charcoal remained. The maximum production of the inhibitor obtained was 100-112 U / ml in the charcoal treated broth.

example 2

[0033] The medium was formulated for the production flasks using lactose, sorbitol, xylan, fructose, maltose and sucrose, all at a concentration of 1% and fermentation process was carried out for 48 hrs. After 48 hrs. the production of inhibitor obtained was maximum of 98 U / ml in presence of sucrose.

example 3

[0034] In this example the fermentation medium used in example 1 was supplemented with amino acids for checking the effect on the production of the inhibitor. The amino acids used in the production flasks were alanine, arginine, asparagine, cystein, glutamic acid, glycine, histidine, proline and serine, all at a final concentration of 0.5%. After 48 hours, the product was isolated and the results are tabulated in Table 1 given herein below. It is observed that maximum production of the inhibitor of 105 U / ml was obtained in presence of asparagine.

TABLE 1Name of the aminoInhibitor ProductionSI. No.acidIn U / ml1.Alanine552.Arginine723.Asparagine1054.Cystein365.Histidine446.Glutamic acid557.Glycine618.Proline89.Serine16

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Abstract

The present invention relates to a novel strain of alkalothermophilic Bacillus sp. isolated from a hot spring at Vajeshwari, District Thane, The State of Maharashtra, India and deposited at American Type Culture Centre (ATCC), bearing accession No. PTA 972, said strain of Bacillus sp. having the following characteristics (i) aerobic, (ii) gram positive, (iii) motile, (iv) spore forming, (v) capable of growing in a alkaline medium at pH 8-10, and (vi) exhibiting negative reaction towards production of indole, hydrogen, sulfide, ammonia and urease and positive reaction for hydrolysis of starch, production of catalase, hydrolysis of casein and reduction of nitrate.

Description

[0001] This application is a division of U.S. Ser. No. 10 / 167,871, filed Jun. 12, 2002, which was a division of U.S. Ser. No. 09 / 541,445 filed Mar. 31, 2000, now U.S. Pat. No. 6,448,060 B1 issued Sep. 10, 2002.TECHNICAL FIELD [0002] This invention relates to a novel strain of an alkalothermophilic Bacillus sp. More particularly the invention relates to a process for the preparation of a low molecular weight peptidic aspartic acid protease inhibitor using a novel strain of alkalothermophilic Bacillus (AT Bacillus sp.) deposited at American Type Culture Collection (ATCC) and bearing Accession Number PTA-972. BACKGROUND OF THE INVENTION [0003] Proteases are responsible either directly or indirectly for all bodily functions including cell growth, nutrition, differentiation and apoptosis. They also play a significant role in intracellular and extracellular protein turn over (house keeping and repair), cell migration and invasion, fertilization and implantation (Protease inhibitors, novel...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/22A01N63/50C07K14/81
CPCA01N63/00C12R1/07C07K14/8142A01N63/50A01N63/22C12R2001/07C12N1/205
Inventor DASH, CHANDRAVANUPHADTARE, SANGITA UDAYAHMAD, ABSARDESHPANDE, VASANTI VISHNURAO, MALA BALCHANDRA
Owner DASH CHANDRAVANU
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