Electrospun electroactive polymers for regenerative medicine applications

a technology of electroactive polymers and regenerative medicine, applied in the field of electroactive polymers for regenerative medicine applications, can solve the problems of poor quality reparative tissue, hyaline cartilage formation, and failure to restore a normal articular surfa

Inactive Publication Date: 2009-12-31
NEW JERSEY INSTITUTE OF TECHNOLOGY
View PDF22 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Orthopedic management of lesions to articular cartilage remains a persistent problem for the orthopedist and patient because articular cartilage has a limited intrinsic ability to heal.
Implantation of scaffolds alone leads to a poor quality reparative tissue.
Chondrocytes implanted either alone or in combination with a scaffold have failed to restore a normal articular surface, and the hyaline cartilage formed early on in response to chondrocyte-containing sc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrospun electroactive polymers for regenerative medicine applications
  • Electrospun electroactive polymers for regenerative medicine applications
  • Electrospun electroactive polymers for regenerative medicine applications

Examples

Experimental program
Comparison scheme
Effect test

example 1

Fabrication Of Piezoelectric Tissue Engineering Scaffolds

[0051]The present invention makes use of fibers formed from a permanently piezoelectric poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer. The PVDF-TrFE copolymer was fabricated into a nanofibrous scaffold using an electrospinning technique.

[0052]The electrospinning process is affected by varying the electric potential, flow rate, solution concentration, capillary-collector distance, diameter of the needle, and ambient parameters like temperature. PVDF-TrFE and PLLA were electrospun into fibers according to commonly used optimization procedures whereby porosity, surface area, fineness and uniformity, diameter of fibers, and the pattern thickness of the sheet could be manipulated. See, e.g., Greiner, A. et al Angew Chem. Int. Ed. Engl. 46: 5670 (2007).

[0053]The electrospinning setup used herein is described in U.S. patent application Ser. No. 11 / 291,701, which is incorporated herein by reference. It is comprised...

example 2

Characterization of the Electrospun PVDF-TrFE Fibers

[0055]Structure and piezoelectric activity were examined by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), thermally stimulated current (TSC) spectroscopy, X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR). Comparisons were made between PVDF-TrFE polymer powder and electrospun PVDF-TrFE fibers.

[0056]The fiber diameter of electrospun PVDF-TrFE fibers was characterized using Scanning Electron Microscopy (SEM) according to established methods and compared to poly L-lactic acid (PLLA) meshes. FIG. 1 shows that the resulting fibrous meshes had an average fiber diameter of 970±480 nm, with uniform fiber morphologies having no beading, as characterized by scanning electron microscopy. The fiber mats were free of droplets.

[0057]Thermally stimulated current (TSC) spectroscopy is widely used to understand dielectric relaxation in complex solid systems. TSC is based on the ability of polar ...

example 3

PVDF-TrFE Fiber Mats Support Stem Cells

[0071]Three studies were conducted to establish that the PVDF-TrFE fiber mesh can be used as a scaffold to support stem cells or other cell types

[0072]Materials and Methods

[0073]1. Cells

[0074](a) Cell line model for neuronal differentiation. When treated with nerve growth factor (NGF), PC12 cells, a cell line derived from a pheochromocytoma of the rat adrenal medulla, stop dividing, grow long neurites, and undergo terminal differentiation, which makes this cell line a useful model system for neuronal differentiation.

[0075]PC12 cells (ATCC number CRL-1721) were seeded at 3×103 cells per cm2 culture dish and maintained in ATCC formulated F-12K medium containing 1.5% fetal bovine serum and 15% horse serum. Cultures were maintained at 37 C, 95% air, 5% CO2 atmosphere. For induction of the neuronal phenotype, 50 ng / ml NGF (Chemicon) was added to the medium at the start of the culture and maintained throughout the duration of the culture. The term “i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Volumeaaaaaaaaaa
Fractionaaaaaaaaaa
Biocompatibilityaaaaaaaaaa
Login to view more

Abstract

Due to the size and complexity of tissues such as the spinal cord and articular cartilage, specialized constructs incorporating cells as well as smart materials may be a promising strategy for achieving functional recovery. Aspects of the present invention describe the use of an electroactive, or piezoelectric, material that will act as a scaffold for stem cell induced tissue repair. Embodiments of the inventive material can also act alone as an electroactive scaffold for repairing tissues. The piezoelectric material of the present invention acts as a highly sensitive mechanoelectrical transducer that will generate charges in response to minute vibrational forces.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The application claims the benefit of U.S. Provisional Patent Application Ser. No. 61 / 039, 214 filed Mar. 25, 2009, entitled “Electrospun Electroactive Polymer for Regenerative Medicine Applications”, the entire disclosure of which is incorporated herein by referenceFIELD OF THE INVENTION[0002]The present invention relates to the use of a synthetic electroactive, or piezoelectric, biomaterial useful as an electroactive scaffold for repairing tissues. The scaffold may be used alone or in combination with cells as scaffold for tissue repair or regeneration.BACKGROUND OF THE INVENTION[0003]Tissue engineering is the application of principles and methods of engineering and life sciences toward a fundamental understanding and development of biological substitutes to restore, maintain and improve human tissue functions.[0004]Orthopedic management of lesions to articular cartilage remains a persistent problem for the orthopedist and patient becau...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/08
CPCA61L27/16A61L27/3834A61L27/3895A61L2400/12C12N2533/30C12N5/0068C08L27/16A61L27/383C12N5/0619C12N2501/13C12N2506/1307C12N2506/1353
Inventor ARINZEH, TREENA L.WEBER, NORBERTJAFFE, MICHAEL
Owner NEW JERSEY INSTITUTE OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap