Ingestible compositions containing extracts
a technology of extracts and compositions, applied in the field of ingestion compositions containing, can solve the problems of reducing elasticity and resilience, reducing the health of gums, and reducing resistance to pressure, and achieve the effect of enhancing elasticity or structural integrity of skin
Examples
example 1
Extract Preparations
[0045]The following is a description of the preparation of various extracts of the present invention. As used in the subsequent Examples, the weight percentage of extract refers to the weight of the liquid extract.
A: Malva Sylvestris Extract Preparation.
[0046]Malva sylvestris (whole dried flowers) was purchased from Botanic Choice (Hobart, Ind.) or Bilek (Troyan, Bulgaria). Ten grams of whole flowers were placed in 200 ml cold water, and brought to boiling in a sealed container. After the appearance of the boiling bubbles, the container was immediately withdrawn from the heating source, covered, and stored at room temperature for from about 1 hour to about 12 hours, with occasional agitation. The extract was then filtered through gauze, and excess liquid was squeezed manually from herbs to maximize the extract yield. The extract was either used as is or was further filtered through 22-micrometer 250 ml filtering unit from Nalgene (Rochester, N.Y.), under vacuum.
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example 2
Enhancement of Elastin Promoter Activity
[0060]Rat cardiac myoblasts H9C2 were purchased from ATCC (Manassas, Va.). Cultures were maintained in Dulbecco's modified Eagle's medium (DMEM, Invitrogen Life Technologies, Carlsbad, Calif.) supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 units / ml penicillin, and 50 μg / ml streptomycin (Invitrogen life technologies, Carlsbad, Calif.).
[0061]Cell cultures were transiently transfected with the elastin promoter-luciferase reporter construct (Elp2.2, a 2.2 kb elastin promoter fragment from nt −2267 to nt +2, driving the firefly luciferase gene, which was obtained from Promega, Madison Wis.). DNA was prepared by Qiagen Maxi columns (Qiagen Valencia, Calif.). In all transfections, a construct with the thymidine kinase promoter and the Renilla luciferase reporter gene (pRL-TK, Promega, Madison Wis.) was included as an internal control. Cells were plated at 4×104 in each well of a 24-well plate (Corning Incorporated, Corning, N.Y.) in gr...
example 3
Protection from Elastase Degradation
[0063]Human leukocyte elastase (HLE) was purchased from Sigma (St. Louis, Mo.), and reconstituted at 1 unit / ml in phosphate buffered saline (PBS, Invitrogen life Technologies, Carlsbad, Calif.). Soluble bovine neck ligament elastin labeled with BODIPY FL dye was purchased from Molecular Probes, Inc. (Eugene, Oreg.), such that the fluorescence was quenched in the conjugate, and could be activated upon elastase digestion. Human leukocyte elastase (0.0625 U / ml), elastin substrate (25 μg / ml), and increasing concentrations of test material were incubated for one hour at room temperature. Fluorescence was measured at excitation at 490 nm and emission at 520 nm using a fluorescent plate reader Gemini from Molecular Devices (Sunnyvale, Calif.). Background fluorescence of substrate alone had been subtracted from each measurement.
[0064]Two batches of Cotinus coggygria extracts, prepared according to Example 1B, were averaged in the experiment, with data pre...
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Description
Claims
Application Information
- IPC
- A61K36/22; A61P17/00
- CPC
- A01N43/653; A61K36/185; A61K36/22; A61K36/28; A61K36/45; A61K36/53; A01N25/00; A01N51/00
- Inventors
- STONE, VIOLETTA IOTSOVA; ZHAO, RENBIN