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Coriolus versicolor extracts, methods of isolating biologically-active compounds, and uses thereof

a technology of biologically active compounds and extracts, which is applied in the separation/purification of carboxylic acid esters, fatty-oils/fats, drug compositions, etc., can solve the problem of limited knowledge of the precise mechanism by which it exerts its pharmacological action, and achieve the effect of reducing or ameliorating the metastasis or invasiveness of tumors

Inactive Publication Date: 2013-10-17
PURAPHARM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a new invention that can be used to treat various types of cancer, including brain, nasopharyngeal carcinoma, breast, lung, leukemia, lymphoma, colon, liver, stomach, esophageal, bladder, and gastric cancer. The invention is also able to reduce the spread or invasiveness of tumor or cancer cells.

Problems solved by technology

While C. versicolor has a long history of empiric uses, there is still limited knowledge about the precise mechanism by which it exerts its pharmacological action.

Method used

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  • Coriolus versicolor extracts, methods of isolating biologically-active compounds, and uses thereof
  • Coriolus versicolor extracts, methods of isolating biologically-active compounds, and uses thereof
  • Coriolus versicolor extracts, methods of isolating biologically-active compounds, and uses thereof

Examples

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example 1

Preparation of Coriolus versicolor Extract

[0180]This Example illustrates preferred extraction schemes for preparing C. versicolor extracts.

[0181]FIG. 1A illustrates one embodiment of the extraction scheme. Briefly, raw materials of C. versicolor were macerated in 12-fold volume of EtOH, extracted for 1 hr with continuous sonication at room temperature, and centrifuged to yield ethanol extract and residues. The residues were macerated in 10-fold volume of EtOH and the extraction procedure was repeated twice as shown in FIG. 1A. The extracts were collected, combined, and evaporated to dryness under vacuum to produce granules comprising C. versicolor ethanol extract.

[0182]In an embodiment, raw materials of C. versicolor were macerated in ethanol for 18 hrs, and centrifuged to yield the ethanol extract and residues.

[0183]In an embodiment, milli-Q water is used as the solvent for preparing C. versicolor water extract. Briefly, raw materials of C. versicolor were macerated in 15-fold volu...

example 2

High Performance Liquid Chromatography Analysis of Coriolus versicolor Extract

[0187]This Example analyzes the chemical fingerprints of the C. versicolor extract by high performance liquid chromatography (HPLC). The C. versicolor extract was obtained using the extraction schemes illustrated in FIG. 1A, 1B, or by macerating the raw material of C. versicolor in ethanol for 18 hrs.

[0188]Briefly, one hundred ug / uL of the EtOH extract was subject to high performance liquid chromatography (HPLC) analysis using an Agilent 1200 series HPLC system (Binary Pump SL, G1312B) equipped with a PDA detector (G1315C) and an autosampler (G1367C). The chromatographic column (4.6×250 mm) was packed with ODS-bonded silica gel (Lichrospher 100 RP C18, EC 5 um), and the column temperature was maintained at room temperature during the separation.

[0189]Five microliters of the C. versicolor extract was injected into the HPLC system. HPLC was performed at a flow rate of 1.0 ml / min using a mixture of water and ...

example 3

Gas Chromatography-Mass Spectrometry Analysis of Coriolus versicolor Extract

[0190]This Example further analyzes the chemical fingerprints of the C. versicolor extract by gas chromatography-mass spectrometry (GC-MS). The C. versicolor extract was obtained using the extraction scheme illustrated in FIG. 1A or by macerating the raw material of C. versicolor in ethanol for 18 hrs.

[0191]The C. versicolor extract was subjected to silylation before analysis by GC-MS. In brief, 100 ul of the extract (30 ug / μL) in acetonitrile was transferred to a 1 ml reaction vial (Alltech), followed by the addition of 50 ul of pyridine and 50 ul of a derivatizing agent BSTFA [N,O-bis (trimethylsilyl) trifloroacetamide], which reacts with a wide range of polar compounds, thereby replacing labile hydrogen atoms of the polar compounds with a —Si(CH3)3 group. After incubation at 70° C. for 2 hrs, the mixture was ready for GC-MS analysis.

[0192]The mixture was analyzed by GC-MS using (GC: Agilent, 7890A; MS: Ag...

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Abstract

The subject invention provides efficient and convenient methods of isolating 9-oxo-10E, 12E-octadecadienoic acid methyl ester (9-KODE methyl ester) from C. versicolor. In addition, the subject invention provides therapeutic uses of Coriolus versicolor extracts, biologically-active chemical substituents isolated from C. versicolor, as well as 9-KODE methyl ester and related compounds. In a preferred embodiment, the subject invention can be used to inhibit the metastatic spread of cancer cells.

Description

[0001]This application claims the benefit of U.S. provisional application Ser. No. 61 / 622,846; filed Apr. 11, 2012, which is incorporated herein by reference in its entirety.BACKGROUND OF THE INVENTION[0002]Coriolus versicolor, also known as Agaricus versicolor, Boletus versicolor, Polyporus versicolor, Polystictus versicolor, Poria versicolor, Trametes versicolor, Yun-Zhi (Chinese), Kawaratake (Japanese), and “turkey tail” (North America), belongs to the Basidiomycetes class and Polyporaceae family. It is widely distributed throughout the world, where more than 120 different strains have been identified in the wooded temperate zones of Asia, Europe, and North America.[0003]The medicinal value of C. versicolor was first recorded in Compendium of Materia Medica (Compendium Medica) by Li Shi Zhen during the Ming Dynasty (1368-1644AD) in China. According to Compendium Medica, C. versicolor (Yun-Zhi), if consumed regularly, can invigorate vital energy, maintain one's optimal weight, pro...

Claims

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Application Information

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IPC IPC(8): C11B1/10
CPCC11B1/10A61K31/231C07C67/48C07C67/58C07C69/738C11C1/002Y02P20/582A61P1/04A61P1/16A61P11/00A61P11/02A61P11/04A61P13/10A61P15/00A61P25/00A61P35/00A61P35/02A61P35/04A61P43/00Y02A50/30A61K31/191A61K36/07C07C59/58
Inventor LAU, ALLAN SIK YINYANG, LAI HUNG CINDYCHIK, STANLEY CHI CHUNG
Owner PURAPHARM
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