The invention provides a quick hot-line by-pass jumper removing operation method for a 10kV insulated power distribution line. The operation method is implemented by a work leader, an on-post ground potential operation electrician and two ground operation electricians. A ground potential operation method is adopted and mainly comprises the operation steps of applying for stopping using re-closing equipment, making insulation isolation measures, removing one side phase by-pass jumper, taking insulation isolation measures at a removing position, removing the other side phase by-pass jumper, removing a middle phase by-pass jumper, detecting and fixing the removed by-pass jumpers, removing an insulating device, restoring the re-closing equipment of the line and the like. The quick hot-line by-pass jumper removing operation method for the 10kV insulated power distribution line has the advantages that the operation method is convenient, quick, time-saving and labor-saving, the working efficiency can be greatly improved, the limitations and the defects of the traditional direction operation methods by using an aerial lift device with an insulating arm and insulating sleeves are overcome, the reliability of power supply guarantee is improved and the range of hot-line operation items of a power distribution network is widened.