The invention discloses a preparation method of a
crocodile polypeptide extract and a beverage, and relates to the technical field of polypeptide extraction and beverage preparation, and the preparation method comprises the following steps: pretreatment of
crocodile skin; denaturation treatment: adding a NaHCO3
buffer solution, and treating under a
microwave condition; performing ultrasonic pretreatment; carrying out enzymolysis reaction, namely sequentially carrying out primary enzymolysis of
alkaline protease and secondary enzymolysis of compounding
trypsin and
neutral protease; adding composite
colloid after
enzyme deactivation treatment, wherein the composite
colloid comprises flaxseed gum and
xanthan gum; centrifuging to take supernate, and carrying out fractional separation through
ultrafiltration membranes with the molecular weight
cut-off of 500Da and 1000Da; and concentrating and
drying to obtain polypeptide
powder. According to the method, the polypeptide yield, the beverage stability and the oral
bioavailability are improved; finally, the obtained polypeptide is scientifically matched with
hyaluronic acid and
vitamin C to prepare the beautifying beverage with the effects of remarkably improving
skin stains and fine wrinkles and brightening the
skin color, and the breakthrough of high-value utilization of
crocodile skin resources and
product function integration is realized.