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Siphon nano-biosensing chip and preparing method thereof

A bio-sensing and chip technology, applied in the field of medical testing, can solve the problem that the accurate measurement cannot exceed 20%, and achieve the effect of ideal linearity and repeatability, improved response sensitivity and accuracy, and significant substantive characteristics.

Inactive Publication Date: 2008-07-16
南京剑桥医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be said that this is the main reason why the accurate measurement of existing products on the market cannot break through 20%

Method used

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  • Siphon nano-biosensing chip and preparing method thereof
  • Siphon nano-biosensing chip and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The siphon nano biosensor chip of this embodiment is used for testing blood sugar, figure 1 and figure 2 As shown, it is mainly composed of two adjacent graphite electrodes 2, 3 attached to the substrate 1, and a reference electrode 4 attached to the front ends of the two graphite electrodes respectively (the reference electrode can also completely or partially cover the entire chip graphite electrode) and work The enzyme electrode 5 is formed (the rear end of the enzyme electrode can also be completely or partially covered by other materials with better conductivity than graphite, such as the reference electrode material used). The base material is double-oriented PET (polyethylene terephthalate), and the upper surface of each electrode is covered with a protective transparent, translucent or opaque PET plastic film 6 bonded by an adhesive layer 7 .

[0043] The specific process of its production is as follows:

[0044] 1), preparation of nano-graphite electrode slu...

Embodiment 2

[0075] The basic structure of the biosensor of this embodiment is the same as that of Embodiment 1, and the specific preparation process is as follows:

[0076] 1), preparation of nano-graphite electrode slurry

[0077] 1-1 Dissolve 15% polyacrylic acid and 15% polyamide in 70% mixed solvent (petroleum cracking aromatic organic solvent), and dissolve slowly for 2-3 hours at 50-60°C to prepare a polymer solution;

[0078] 1-2 Fully mix the prepared polymer solution and nano-scale graphite in a three-roll internal mixer, and the shearing force is continuously increased until the particle size of the dispersed phase reaches the nano-scale;

[0079] 1-3 Use mixed solvent to adjust the viscosity to 20-60Pa.s.

[0080] 2), preparation of reference electrode slurry

[0081] 2-1 is the same as 1-1;

[0082] 2-2 Fully mix the prepared polymer solution with 68% nano-scale silver and silver chloride (ratio Ag:AgCl=70:30) in a three-roll internal mixer, and the shearing force is contin...

Embodiment 3

[0097] The basic structure of the biosensor of this embodiment is the same as that of Embodiment 1, and the specific preparation process is as follows:

[0098] 1), preparation of nano-graphite electrode slurry

[0099] 1-1 Dissolve 15-30% methacrylic acid, n-butylacrylic acid copolymer and 5-10% polyvinyl acetate in 70% mixed solvent (petroleum cracking aromatic organic solvent) at 50-60°C , slowly dissolve for 2-3 hours to prepare a polymer solution;

[0100] 1-2 Evenly mix the prepared polymer solution and graphite powder;

[0101] 1-3 Use mixed solvent to adjust the viscosity to 20-60Pa.s.

[0102] 2), preparation of reference electrode slurry

[0103] 2-1 is the same as 1-1;

[0104] 2-2 Fully mix the prepared polymer solution with 50-70% nano-scale silver and silver chloride (ratio Ag:AgCl=60:40) in a three-roll internal mixer, and the shearing force is continuously increased until The particle size of the dispersed phase reaches the nanometer level, and a conductiv...

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Abstract

The invention relates to a biology sensor chip-siphon nm biology sensor chip that is mainly made up of two graphite electrode, reference electrode and working enzyme electrode. The reference electrode disperses metal nm material powder in macromolecule adhering system to from nm aggregation state and nm effective hole. The working enzyme electrode and enzyme active component disperse in macromolecule system to form nm aggregation state and nm effective hole. The invention sharply improves the rapid response and accuracy of the biology sensor and shortens the detecting time. The blood needed to take testing is obviously decreased.

Description

technical field [0001] The invention relates to a biosensor chip and a preparation method of the biochip, belonging to the technical field of medical detection. Background technique [0002] Enzyme biosensors (especially glucose enzyme biosensors) are a rapidly developing new technology for disease detection. Taking the glucose biosensor applied in the organism as an example, its mechanism is: with the help of oxygen, based on the reaction of glucose oxidase catalyzing the oxidation of glucose, the detection purpose is achieved by monitoring the generation of hydrogen peroxide or the consumption of oxygen. Its main disadvantage is that the accuracy of the detection depends on the complete glucose reaction, and the detection cannot be completed in a short time. In addition, the manufacturing process requires a polymer body control coating to eliminate interference from other species, enzyme layers and outer layers, which is more complicated. [0003] In recent years, glucos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/406G01N27/327
Inventor 韩进
Owner 南京剑桥医疗器械有限公司
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