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Method for preparing extractive of olive leaves rich in oleuropein in high purity

A technology of olive leaf extract and oleuropein, which is applied in the field of preparation of olive leaf extract, can solve the problems of unclear active ingredients, large solvent loss, low content, etc., to achieve protection and regeneration, and improve production efficiency and purity, and the effect of improving purity

Inactive Publication Date: 2007-07-25
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yan Yiqian used Chinese olive green fruit and leaves to prepare olive polyphenols, and applied for a patent (CN1333203A). The extraction process is water extraction, alcohol precipitation, and ethyl acetate extraction to prepare 80% olive polyphenols. This process not only consumes a lot of solvents, but also High cost and no oleuropein in the extract
Yang Yinke's patent (200410023233) reports a method for extracting olive leaf extract, which uses water rinsing, chlorine dioxide disinfection, alcohol extraction, concentration and drying. What are the ingredients, and the content is low, and its medicinal use is limited
Therefore, extracting and separating the olive leaf extract rich in high-purity oleuropein from olive leaves, there is no domestic report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A preparation method of an olive leaf extract rich in high-purity oleuropein, comprising the following steps:

[0050] In the first step, the olive leaves are extracted with a polar solvent, wherein the mass ratio of the polar solvent to the olive leaves is (1-30): 1, for example, the ratio can be selected as: 1:1, 4:1, 7:1 , 8:1, 10:1, 14:1, 17:1, 20:1, 22:1, 24:1, 27:1, 28:1, 29:1, 29.5:1, 30:1, extraction The temperature is 20°C-90°C, for example, it can be selected as: 25°C, 35°C, 40°C, 50°C, 60°C, 70°C, 75°C, 80°C, 85°C, the extraction time is 0.5h-72h, the extraction time 0.5h, 1h, 3h, 5h, 6h, 8h, 9.5h, 12h, 14h, 15h, 16h, 17h, 18h, 20h, 30h, 35h, 40h, 55h, 60h, 70h, 71h,

[0051] The extraction method can be one of heat reflux, microwave extraction, and ultrasonic extraction. The heat reflux extraction temperature is 50°C-70°C. For example, the temperature can be selected as: 55°C, 62°C, 65°C, 68°C, 69°C, and the time is 0.5-10 hours, such as 0.5h, 1h, 3h, 5h, ...

Embodiment 2

[0058] Select 10-20-year-old olive trees, take 15kg of fresh olive leaves collected in February, put them in a ventilated and shaded place to dry, and then blow air in an oven below 50°C for 3-20hrs, and measure the moisture content below 5.0%. Oleuropein 2.9%. Take 5kg of dry leaves, crush them into fine powder, add 40kg of petroleum ether (60°C-90°C), extract under reflux at 70°C for 4 hours, add 30kg of petroleum ether (60°C-90°C) for the second extraction, and repeat the above operate. The extracts were combined, and the solvent was recovered under reduced pressure at 50° C. to obtain 240 g of petroleum ether extract, which was olive leaf ointment, with a yield of 4.8%.

[0059] Add 50kg of 60% ethanol aqueous solution to the olive leaves extracted with petroleum ether, heat extraction at 70°C for 3 hours, filter, add 30kg of 50% ethanol aqueous solution for 2 hours, and combine the filtrates for the second extraction. Concentrate under reduced pressure at 65°C to a spec...

Embodiment 3

[0062] Select 3-10-year-old olive trees, take 20kg of fresh olive leaves collected in May, put them in a ventilated and shaded place to dry, and then blow air in an oven below 50°C for 3-20hrs, and measure the moisture content below 5.0%. Oleuropein 5.6%. Take 3kg of dried leaves, crush them into fine powder, add 25kg of 80% ethanol aqueous solution, heat extraction at 70°C for 3 hours, filter, add 20kg of 40% ethanol aqueous solution for 2 hours for the second extraction, and combine the filtrates. Concentrate under reduced pressure at 65°C to a specific gravity of 1.10, add water to dilute to 30kg, adjust the pH to 8-9 with alkali, and centrifuge to precipitate at a speed of 4000 rpm; add deionized water, precipitate and deionized water to the precipitate The mass ratio was 1:5, the precipitate was removed, and the supernatants were combined twice. The clarified liquid is sequentially processed through a ceramic membrane, an ultrafiltration membrane and a nanofiltration mem...

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Abstract

This invention discloses a method for preparing olea leaf extract rich in high-purity oleuropein. The method comprises: extracting olea leaves with polar solvent, centrifuging to obtain the supernatant and the precipitate, performing composite membrane separation to obtain concentrated solution passing through the nanofiltration membrane, adsorbing with an adsorption medium, eluting the adsorption medium to obtain the eluate, vacuum-concentrating, and freeze-drying or spray-drying in vacuum to obtain olea leaf extract rich in high-purity oleuropein (more than 50%). The method adopts composite membrane separation and medium adsorption techniques to replace traditional solvent extraction technique, thus can increase oleuropein purity, save energy and eliminate pollution. The polar solvent can effectively extract oleuropein, and the nonpolar solvent can effectively remove liposoluble substances, thus is advantageous for increasing oleuropein yield and purity.

Description

technical field [0001] The invention relates to a preparation method of an olive leaf extract, in particular to a preparation method of an olive leaf extract rich in high-purity oleuropein. Background technique [0002] Olive (Olea europaea L), also known as foreign olive, is a rare evergreen woody oil plant and fruit tree species in the world. It belongs to the genus Oleaceae Oleaceae in plant classification. Olive is native to countries along the Mediterranean Sea and is a subtropical crop with a cultivation history of more than 6,000 years. The pulp of the oleocanthal contains oil and is used to extract olive oil. [0003] Chinese olive (Canarium album Raeusch) belongs to the genus Olivaceae in terms of plant classification. Chinese olive is also known as green fruit. Its pulp does not contain oil and is widely distributed in subtropical and tropical regions. Olive green fruit is used to make candied fruit and preserved fruit in southern my country. Therefore, the used ...

Claims

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Application Information

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IPC IPC(8): C07H17/04A61K36/185A61K127/00
CPCY02P20/10
Inventor 王成章高彩霞
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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