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Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent

A natural antibacterial agent and alcohol fermentation technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of low profit in the fermentation industry, expensive antibiotics, antibiotic residues, etc., to achieve low prices and no pollution , the effect of promoting growth

Inactive Publication Date: 2010-12-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high price of antibiotics makes the fermentation industry less profitable, and the problem of antibiotic residues and the emergence of drug-resistant bacteria have led countries to formulate corresponding laws to reduce the use of antibiotics

Method used

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  • Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent
  • Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] First take ebony and crush it to 20 mesh fine powder, weigh 30g, add 240mL of deionized water for reflux extraction for 1.5h, collect the supernatant after centrifugation and further concentrate to 60mL, and then sterilize with wet heat to obtain 0.5g / mL ebony Extract (equivalent to 0.5 g of ebony per milliliter of extract). Cultivate rice wine yeast and Lactobacillus fermentum ATCC 14931 (Lactobacillus fermentum ATCC14931 can grow in large quantities within 36h at 30°C, and can survive under>10% (v / v) alcohol (referring to literature: Appl.Environ.Microbiol., 2000 , 66, 4187.) to the logarithmic growth phase. After liquefaction and saccharification of corn syrup, the fermentable sugar can reach ~60mg / ml, and it is divided into 100ml fermentation broth for each shaker bottle with a volume of 250ml, and then yeast Bacteria suspension and lactic acid bacteria suspension, so that yeast and lactic acid bacteria reach ~10 8 CFU / mL (colony forming units per milliliter) and ~...

Embodiment 2

[0019] The embodiment is as in Example 1, except that after adding the same amount of yeast and lactic acid bacteria, 8 mL of ebony extract is added. Samples were taken every 12 hours, and the amounts of alcohol and lactic acid in the samples were determined by high performance liquid chromatography. The alcohol production at the end of the fermentation was measured to be 9.60 mg / ml, while the lactic acid production was 0.10 mg / ml. The corresponding colony counting result is: the number of yeast cells starts from 1.6×10 8 CFU / mL, reaching 3.9×10 after 12h 8 CFU / mL; the initial number of lactic acid bacteria is 0.6×10 8 CFU / mL, 1.6×10 at the end of fermentation 8 CFU / mL.

Embodiment 3

[0021] Embodiment is as embodiment 1, and ebony extract is changed into 15mL. Samples were taken every 12 hours, and the amounts of alcohol and lactic acid in the samples were determined by high performance liquid chromatography. The alcohol production at the end of the fermentation was measured to be 9.80 mg / ml, while the lactic acid production was reduced to 0 mg / mL. The corresponding colony counting result is: the number of yeast cells starts from 1.6×10 8 CFU / mL, reaching 3.2×10 after 12h 8 CFU / mL; the initial number of lactic acid bacteria is 0.6×10 8 CFU / mL, 1.4×10 at the end of fermentation 6 CFU / mL.

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Abstract

The invention relates to a method for utilizing natural antibacterial agent to control alcohol fermentation infected bacteria and provides a method for utilizing the natural antibacterial agent to control the alcohol fermentation infected bacteria, which not only can control the growth of bacterial population and can reduce the amount of organic aid which is produced by bacteria, but also can promote the growth of yeast and can increase the output of alcohol. The method comprises following steps: taking dark plum, crushing the dark plum to 20-40 mesh fine powder, adding deionized water to extract 1-240 min, centrifuging, then, collecting supernatant fluid and further condensing until the content of the dark plum in extracting solution is 0.5-1g / ML, obtaining concentrated solution, obtaining dark plum extracting solution to reserve through moist heat sterilization, and adding the dark plum extracting solution 2%-15% of the fermentation broth amount according to the volume fraction in an alcohol fermentation system. Since the dark plum contains various organic acid, the method has strong inhibitory effect to gram-positive bacteria, dark plum extracts are natural plant extracts, drugresidue is not available, pollution is not available, the cost is low, and the method has economic feasibility and is suitable for industrial production with large scale.

Description

technical field [0001] The invention relates to a method for using natural antibacterial agents to prevent and control alcohol fermentation contamination. Background technique [0002] The fermentation production of fuel alcohol is developing rapidly all over the world. A lot of research is devoted to using low-cost biomass instead of corn as the fermentation substrate, but little attention is paid to the loss caused by bacterial contamination during fermentation. Typical contaminating bacteria that can be encountered in alcohol fermentation plants are: Lactobacillus buchnerii, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus casei, Lactobacillus brevis, Lactobacillus acidophilus, Lactobacillus lindella, Leuconostoc enterococci, Bacillus brevius, Bacillus cereus (see Chinese patent CN 1095415A), acetic acid bacteria and butyric acid bacteria, etc. Lactic acid bacteria are the main polluting bacteria in alcoholic fermentation because they can survive in acidic and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/08C12R1/645C12R1/01
CPCY02P20/54
Inventor 李清彪郑进保何宁王远鹏杨坤孙道华王海涛邵文尧林文爽杨欣黄加乐
Owner XIAMEN UNIV