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Preparation technique for passionflower total flavones and total saponins without alkaloid and cyanogenic glycoside

A preparation process and technology of passionflower are applied in the field of medicinal total flavonoids and total saponin extracts of passionflower, can solve the problems of low extraction rate of total flavonoids, poor water solubility of products, etc., achieve good water solubility, improve sleep, extract high rate effect

Inactive Publication Date: 2011-01-12
SOUTHWEST UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The previous preparation technology of Passiflora plant extract only used a kind of concentration alcohol aqueous solution or water as extractant, as extracting with higher concentration alcohol aqueous solution, the chlorophyll content is higher, and the water solubility of product is poor; Concentrated alcohol aqueous solution or water extraction, the extraction rate of total flavonoids is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) 50kg of fresh Passiflora cauline leaves are blast-dried to water content 20% at 60°C, pulverized into 10 orders, add 80% methanol and mix well, so that fully imbibition;

[0021] (2) Pack into a 100L stainless steel interlayer percolation barrel, press tightly layer by layer, put a piece of filter paper on the raw material, and spread the washed river sand on it;

[0022] (3) Connect the interlayer water bath, keep the percolation temperature at 55°C, add 70% methanol from the top of the percolation barrel, open the valve at the bottom of the percolation barrel, and make the percolation liquid flow out at a speed of 300ml / min;

[0023] (4) After percolating with 150L of 70% methanol, switch to 150L of 50% methanol for percolation, and then use 100L of 30% methanol of pH 11 and 100L of NaOH aqueous solution of pH 11 for percolation, keeping the outflow rate of the percolation liquid at 300ml / min;

[0024] (5) Collect 70% and 50% methanol percolation liquid, recover m...

Embodiment 2

[0031] (1) 100kg of fresh passion fruit stems and leaves are dried in the sun to a moisture content of 25%, pulverized into 5 orders, add 75% ethanol and mix well, so that fully imbibition;

[0032] (2) Pack into a 250L stainless steel interlayer percolation barrel, press tightly layer by layer, put a piece of filter paper on the raw material, and spread the washed river sand on it;

[0033] (3) Connect the interlayer water bath, keep the percolation temperature at 60°C, add 65% ethanol from the top of the percolation barrel, open the valve at the bottom of the percolation barrel, and make the percolation liquid flow out at a speed of 500ml / min;

[0034] (4) After percolation with 300L 60% ethanol, use 300L 40% ethanol for percolation, and then use 200L pH11 20% ethanol and 200L pH11 KOH aqueous solution for percolation, keeping the outflow rate of percolation liquid at 500ml / min ;

[0035] (5) Collect 60% and 40% ethanol percolation liquid, recover ethanol under reduced pres...

Embodiment 3

[0042] (1) 30kg of fresh Passiflora cauline leaves are air-dried to water content 15% at 60° C., pulverized into 5 orders, add 70% n-propanol and mix well, so that fully imbibition;

[0043] (2) Pack into a 200L stainless steel traditional Chinese medicine multifunctional extraction tank, add 150L of 65% n-propanol, stir and extract at 65°C for 1.5 hours, and filter;

[0044] (3) Add 120L of 30% methanol with pH 11 to the filter residue, stir and extract at 65°C for 1 hour, filter, and then use Ca(OH) with pH 11 to filter the residue 2 100L aqueous solution, stirred and extracted at 65°C for 0.5 hours, filtered;

[0045] (4) 65% of the n-propanol extract is recovered under reduced pressure at about 70°C to reach an alcohol concentration of about 20%, and the concentrated solution is saturated with Ca(OH) 2 Adjust the pH of the aqueous solution to 11, cool down to 8°C, and keep standing at 8°C overnight to precipitate chlorophyll and alkaloids;

[0046] (5) filter, wash the f...

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Abstract

The preparation technique of passionflower total flavone and total saponin which does not contain alkaloid and cyanogentic glycoside has the steps as follows: (1) passionflower cauline leaf is dried to have 10 percent to 35 percent of water content under the temperature that is lower than 70 DEG C to be crushed into crude powder; (2) the crude powder is wetted and expended by lower alcohol aqueous solution that is more than 70 percent and is carried out by thermal diacolation by the lower alcohol aqueous solution with gradual lower concentration in sequence; (3) the diacolation liquid with the alcohol concentration higher than 40 percent is depressurized for recovering alcohol till the alcohol concentration is 15 percent to 25 percent, and condensed liquid is basified to pH11; (4) chlorophyll is filtered, filtrate is combined with the diacolation liquid with the alcohol concentration lower than 40 percent, pH is adjusted to 5 to 7, all the alcohol is depressurized and vaporized, and pH is further adjusted to 3; (5) the passionflower total flavone can be obtained by being filtered and vacuum drying; (6) the filtrate is desalted by an ion exchange resin method, hygroscopic agent is added into permeate liquid to be sprayed and dried, and the passionflower total saponin can be obtained. The preparation technique has simple process flow, high extracting rate and little dosage of organic solvent and does not pollute the environment, and the product has little toxic and side effect.

Description

Technical field: [0001] The invention relates to an extract of total flavonoids and total saponins of Passiflora medicinalis free from alkaloids and cyanogenic glycosides and their extraction and preparation process. Background technique: [0002] The stem and leaf extract of Passiflora edulis Sim. has been used as a therapeutic medicine for hundreds of years. In South America, Passiflora is used as a sedative, diuretic, anthelmintic, antidiarrheal, stimulant, tonic, and also in the treatment of high blood pressure, menopausal syndrome, and colic in infants. In Nagaland, India, the fresh leaves of passionflower are boiled with a small amount of water, and the extract can be used to treat dysentery and high blood pressure. Passiflora extract currently on the market is mainly used for anti-anxiety, sedation, treatment of insomnia, pain relief and other purposes. [0003] The main chemical composition of passionflower stems and leaves includes a large number of flavonoids, es...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P25/04A61P25/20A61P25/22A61K127/00A61K135/00
Inventor 邓君李莉刘光铨
Owner SOUTHWEST UNIV
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