Method for improving purity of raffinose

A raffinose and purity technology, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of easy fouling of separation membranes, long process routes, and many steps, and achieve strong selective adsorption. The effect of industrialized production and simplified purification process

Active Publication Date: 2009-03-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of lead acetate will bring heavy metal residues, while the investment of ultrafiltration equipment is relatively large, and there are problems that the separation membrane is easy to pollute and has a short life.
[0010] The usual process of removing monosaccharides and sucrose in the primary product of raffinose is completed simultaneously in the process of crystallization or precipitation, which leads to the need for multiple recrystallizations to obtain high-purity raffinose, which will inevitably reduce the yield of raffinose. Total yield increases production cost and

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0041] Hereinafter, the content of the present invention will be explained in detail through examples, but the present invention is not limited to these. Example 1: Preparation of raffinose decolorizing liquid

[0042] (a) Absorbent cottonseed meal (wherein, the mass percentage of raffinose is 3.9%) is sieved through a 1.8mm sieve, and 435 grams are weighed and loaded into the percolation column (Φ3.5cm×70cm). After the filling is even Its bulk volume is about 560 ml. Then add 350 ml of 75% ethanol aqueous solution to the percolation column, soak it at 55°C for 30 minutes to fully swell the absorbent cottonseed meal, open the outlet valve of the percolation column, and self-infiltrate at the same time. The extraction solvent was continuously added to the top of the column, and the flow rate was controlled to 168 ml / hour until the volume of the collected percolation extract was 1900 ml. After analysis of the percolate, the solid content (solid content refers to the percentage of th...

Example Embodiment

[0048] Example 2: Preparation of crude raffinose

[0049] Load the pretreated HZ816 resin into an adsorption column with a size of Φ5.6cm×97cm, the column bed volume BV is about 2400mL, and the resin is about 1700g. The sample loading liquid is the raffinose decolorizing liquid prepared according to the process of Example 1, wherein the solid content is 10%, and the mass percentage of raffinose calculated on a dry basis is 40%. The raffinose decolorizing solution 4BV is continuously introduced into the fixed bed adsorption column at a flow rate of 1.5 BV / h. The effluent collected during the sample loading process also contains a small amount of raffinose, which can be directly recycled. After loading the sample, first rinse the adsorption column with 2BV deionized water at a flow rate of 2.5BV / h to remove the decolorizing liquid remaining in the adsorbent voids in the adsorption column, and the obtained washing liquid can be recycled; then use 2BV with a volume percentage of 40 % ...

Example Embodiment

[0052] Example 3: Preparation of crude raffinose

[0053] Load the pretreated HZ802 resin into an adsorption column with a size of Φ5.6cm×97cm, the column bed volume BV is about 2400mL, and the resin is about 1700g. The sample loading liquid is the raffinose decolorizing liquid prepared according to the process of Example 1, wherein the solid content is 10%, and the mass percentage of raffinose calculated on a dry basis is 48%. The raffinose decolorizing solution 3BV is continuously introduced into the fixed bed adsorption column at a flow rate of 1.0 BV / h. The effluent collected during the sample loading process also contains a small amount of raffinose, which can be directly recycled. After loading the sample, first rinse the adsorption column with 1.5BV deionized water at a flow rate of 1.0BV / h to remove the decolorizing solution remaining in the adsorbent voids in the adsorption column, and the obtained washing solution can be recycled; then use 4BV volume percentage as The 20...

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PUM

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Abstract

The invention discloses a method for improving the purity of raffinose. A fixed bed adsorption separation method is adopted to implement the separation of the raffinose from impurities such as salt, protein, monose, sucrose and the like by utilizing the one-step adsorption separation, so that the mass percentage content of the raffinose in the dry basis is increased from 30%-50% to above 90%, and simultaneously, the loss rate of the raffinose is not more than 3%. The method has the advantages of simple process, convenient adsorbent regeneration, low production cost, short separation period, and easy implementation of the industrial production.

Description

technical field [0001] The invention relates to a method for improving the purity of raffinose, belonging to the technical field of adsorption and separation. Background technique [0002] Raffinose, also known as Melitriose and raffinose, is composed of galactose, glucose and fructose, and is an oligosaccharide and a non-reducing sugar. Research has found that raffinose is an excellent nutrient source for beneficial bacteria such as Bifidobacterium and Lactobacillus acidophilus in the human intestinal tract, which can promote their proliferation, thereby inhibiting the growth of spoilage bacteria in the intestinal tract and reducing the formation of toxic fermentation products . Bifidobacteria ferment raffinose to produce a large amount of short-chain fatty acids, which can stimulate intestinal peristalsis, increase stool moisture and maintain a certain osmotic pressure, thereby preventing constipation. In addition, raffinose can improve the digestive function of the huma...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/06
Inventor 任其龙鲍宗必朱魏吴彩娟苏云邢华斌苏宝根
Owner ZHEJIANG UNIV
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