Method for producing open tubular electrophoresis column coating with nano titanic oxide and uses thereof

A nano-titanium dioxide, electrophoresis column technology, applied in chemical instruments and methods, separation methods, other chemical processes, etc., can solve the problems of uncontrollable reaction rate, uneven mixing of reactants, high cost of metal alkoxides, and reduce non-specificity. The effect of adsorption, good chemical bonding strength and simple and easy preparation method
CN101406828AInactive Publication Date: 2009-04-15QIQIHAR UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QIQIHAR UNIVERSITY
Publication Date
2009-04-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing an open-tube electrophoretic column coated with nanometer titanium dioxide, as well as application thereof. In an etched capillary column, an open-tube capillary electrophoretic column coated with the nanometer titanium dioxide is prepared by a water (solvent) thermal method. The preparation method is characterized in simplicity, coatings insusceptible to fall off, and the like, and is suitable for the selective enrichment and separation of functionalized phosphorylated polypeptide and protein in complex biological samples. Titanium dioxide molecules are combined with silicon hydroxyl on the surface of the inner wall of a capillary through chemical bonds, and nanometer titanium dioxide particles are uniform in grain, complete in coatings and capable of effectively reducing the nonspecific adsorption effect to the protein and other macromolecule samples. The surface properties and micropore structures of the coatings can be conveniently controlled by changing the categories and proportion of middle pore-forming agents in hydrothermal mixtures, so as to obtain a phosphorylated polypeptide capillary column with adjustable pore diameter and specific surface area.
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Description

【Technical field】

[0001] The invention relates to the technical field of open-tube electrophoresis columns, in particular to a preparation method and application of an open-tube electrophoresis column coated with nano-titanium dioxide. 【Background technique】

[0002] Protein phosphorylation plays an important regulatory role in life activities, so it is also a hot spot in the research of post-translational modification proteome. However, large-scale analysis of phosphorylated proteins is technically challenging, and the main technical challenge is analytical sensitivity.

[0003] Due to the low content and low stoichiometric value of phosphorylated proteins in organisms, only a small part of most proteins in the cell is phosphorylated. Generally, the phosphorylated part of a phosphorylated protein accounts for only 10% of the total amount, so Even when the protein is expressed at a relatively high level, analysis of the phosphorylated portion of the protein is difficult. T...

Claims

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