Method for producing open tubular electrophoresis column coating with nano titanic oxide and uses thereof

A nano-titanium dioxide, electrophoresis column technology, applied in chemical instruments and methods, separation methods, other chemical processes, etc., can solve the problems of uncontrollable reaction rate, uneven mixing of reactants, high cost of metal alkoxides, and reduce non-specificity. The effect of adsorption, good chemical bonding strength and simple and easy preparation method

Inactive Publication Date: 2009-04-15
QIQIHAR UNIVERSITY
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The uniform precipitation method not only improves the disadvantages of uneven mixing of reactants and uncontrollable reaction rate in the pr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing open tubular electrophoresis column coating with nano titanic oxide and uses thereof
  • Method for producing open tubular electrophoresis column coating with nano titanic oxide and uses thereof
  • Method for producing open tubular electrophoresis column coating with nano titanic oxide and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of open-tube electrophoresis column: Stir 1ml of absolute ethanol, 0.34g of tetrabutyl titanate, and 0.2875g of polyethylene oxide-polypropylene oxide-polyethylene oxide, and pass N2 to remove dissolved oxygen. Add HCl solution to adjust the pH to 1-2, heat the reaction kettle to 130°C, and react for 12 hours. Finally, rinse with ethanol to remove the porogen. figure 1 Electron micrograph of the combined surface of SiO2-TiO2 coated nano-titanium dioxide open-tube electrophoresis column.

Embodiment 2

[0040] Preparation of open-tube electrophoresis column: Stir 1ml of absolute ethanol, 0.34g of tetrabutyl titanate, and 0.63g of polyoxyethylene-polyoxypropylene-polyoxyethylene block copolymer evenly, and pass N 2 After removing dissolved oxygen, add HCl solution to adjust the pH to 1-2, heat the reactor to 150°C, and react for 11 hours. Finally, rinse with ethanol to remove the porogen. figure 2 Enrichment mass spectrum of β-casein hydrolysis product for titanium dioxide open-tube column.

Embodiment 3

[0042]Preparation of open-tube electrophoresis column: Stir 1ml of absolute ethanol, 0.34g of tetrabutyl titanate, 0.2875g of polyethylene oxide-polypropylene oxide-polyethylene oxide, and pass N 2 After removing the dissolved oxygen, add HCl solution to adjust the pH to 1-2, heat the reactor to 130°C, and react for 12 hours. Finally, rinse with ethanol to remove the porogen. image 3 The mass spectrum of α-casein (5picomoles) enzymatic hydrolysis product was enriched for titanium dioxide open-tube column.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing an open-tube electrophoretic column coated with nanometer titanium dioxide, as well as application thereof. In an etched capillary column, an open-tube capillary electrophoretic column coated with the nanometer titanium dioxide is prepared by a water (solvent) thermal method. The preparation method is characterized in simplicity, coatings insusceptible to fall off, and the like, and is suitable for the selective enrichment and separation of functionalized phosphorylated polypeptide and protein in complex biological samples. Titanium dioxide molecules are combined with silicon hydroxyl on the surface of the inner wall of a capillary through chemical bonds, and nanometer titanium dioxide particles are uniform in grain, complete in coatings and capable of effectively reducing the nonspecific adsorption effect to the protein and other macromolecule samples. The surface properties and micropore structures of the coatings can be conveniently controlled by changing the categories and proportion of middle pore-forming agents in hydrothermal mixtures, so as to obtain a phosphorylated polypeptide capillary column with adjustable pore diameter and specific surface area.

Description

【Technical field】 [0001] The invention relates to the technical field of open-tube electrophoresis columns, in particular to a preparation method and application of an open-tube electrophoresis column coated with nano-titanium dioxide. 【Background technique】 [0002] Protein phosphorylation plays an important regulatory role in life activities, so it is also a hot spot in the research of post-translational modification proteome. However, large-scale analysis of phosphorylated proteins is technically challenging, and the main technical challenge is analytical sensitivity. [0003] Due to the low content and low stoichiometric value of phosphorylated proteins in organisms, only a small part of most proteins in the cell is phosphorylated. Generally, the phosphorylated part of a phosphorylated protein accounts for only 10% of the total amount, so Even when the protein is expressed at a relatively high level, analysis of the phosphorylated portion of the protein is difficult. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B01J20/281B01J20/06B01D15/22G01N30/60G01N27/453
Inventor 李英杰高立娣苏立强吕仁江
Owner QIQIHAR UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap