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SiO2/Au nano material with nucleocapsid structure-biological protein medicament complex and preparation method thereof

A technology of drug complexes and nanomaterials, which is applied in the direction of drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., to achieve the effect of improving the therapeutic effect

Inactive Publication Date: 2009-04-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current use of Endostar is combined with other conventional drugs in order to enhance the efficacy of the drug, and there is no report on the combined use of Endostar drugs and inorganic nanomaterials

Method used

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  • SiO2/Au nano material with nucleocapsid structure-biological protein medicament complex and preparation method thereof
  • SiO2/Au nano material with nucleocapsid structure-biological protein medicament complex and preparation method thereof
  • SiO2/Au nano material with nucleocapsid structure-biological protein medicament complex and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] SiO 2 / Preparation of Au core-shell nanomaterials

[0033] a. Preparation of monodisperse nano-silica spheres: Take 10ml of high-purity water and add it to an Erlenmeyer flask, add 25ml of absolute ethanol, 5ml of ammonia water, stir evenly with a magnetic force, add 1.5ml of ethyl orthosilicate, and stir at room temperature for 12 hours to obtain SiO with a particle size of 120nm 2 Nanoparticles;

[0034] b. Preparation of aminated silica nanoparticles: add 80ul aminopropyltrimethoxysilane, stir for 10min, let stand for 8h, boil in a water bath for 2h, and centrifugally wash at a speed of 6000r / min to obtain aminated silica Nanoparticles, TEM morphology as shown in figure 1 As shown, the distribution is relatively uniform;

[0035] c. Preparation of the silica gold seed solution: mix the gold colloid with a particle size of 1-2nm and the aminated silica nanoparticles obtained in step b, ultrasonicate the contact for 30s, sink at 4°C for 8h, and dissolve at 300Or / ...

Embodiment 2

[0044] Step d adopts the mol ratio of chloroauric acid and formaldehyde to be 1:100, 1:150, 1:200, 1:250 respectively; Or 1:300, all the other steps are with embodiment 1, and the obtained product SiO 2 / Au core-shell structure nanomaterials-Enstar composite measured surface plasmon resonance absorption spectrum as shown in Figure 5 As shown, it is found that the absorption peak of the product moves to the long-wave direction with the increase of the amount of reducing agent formaldehyde, and the absorption peak red shifts by 130nm. Curves a, b, c, d and e represent respectively the absorption curves when the mol ratio of chloroauric acid and formaldehyde is 1:100, 1:150, 1:200, 1:250 and 1:300, illustrating that this reaction can Further control the absorption peak position by adjusting the reduction rate, this phenomenon has not been reported in the literature.

Embodiment 3

[0046] With embodiment 1 gained SiO 2 / Au-vascular endostatin complex is used for in vitro lung cancer cell killing test: using an infrared laser with a wavelength of 808nm, the irradiation time is 7 minutes, and the lung cancer cell inhibition rate is measured by the MTT method. The results are as follows Figure 6 Shown: when using pure SiO 2 / Au core-shell structure nanomaterials in vitro lung cancer cell killing test, its inhibition rate of lung cancer cells was 30%; while SiO 2 When the / Au core-shell structure nanomaterial-vascular endostatin complex is used in the in vitro lung cancer cell killing test, its inhibition rate on lung cancer cells is increased to 88% under the same conditions. Visible, SiO 2 / Au-endostatin complex does have a very significant effect on the inhibition rate of cancer cells in vitro.

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Abstract

The invention relates to a composite of SiO2Au nuclear shell structure nano material and biological protein medicament. The composite is prepared through grafting the SiO2Au nuclear shell structure nano material with the biological protein medicament by Au-S bond, and closing rest non-active sites of the SiO2Au nuclear shell structure nano material by sulphane polyethylene glycol. A preparation method comprises the following steps: (1) making amino polyethylene glycol and 2-imino sulphane according to a molar ratio of 1 to 1 reacted in potassium carbonate solution for 1 hour, dialyzing and purifying the reagents by secondary distilled water, and obtaining the sulphane polyethylene glycol; and (2) mixing the SiO2Au nuclear shell structure nano material, the biological protein medicament and the 2-imino sulphane in the potassium carbonate solution, standing over night, adding the mixture into the sulphane polyethylene glycol obtained in step (1) for reaction under the condition of weak alkali buffer solution, so as to obtain the composite. The composite compounds physical therapy of inorganic nano particles and biological therapy function of protein medicament, remarkably improves treatment effect for tumor, and can be used a new anticancer medicament.

Description

technical field [0001] The present invention relates to a SiO 2 The invention relates to a nanomaterial-biological protein drug compound with Au core-shell structure and a preparation method thereof, which belong to the field of bionano technology. Background technique [0002] In recent years, the application of nanotechnology in biomedicine has a very important position and bright prospects. The application of nanotechnology in the field of biomedicine mainly includes the following fields: drug release and targeting; cell and protein detection; disease Diagnosis and treatment; medical nanorobots, etc. [0003] The discovery of near-infrared absorption properties of core-shell structure noble metal nanomaterials has attracted extensive attention in related fields such as physics, chemistry, and biology, and people have seen the broad application prospects of metal nanomaterials in the field of biomedicine. The near-infrared region (800-1200nm) is the transmission window f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/02A61P35/00A61K47/60
Inventor 满石清张浩然徐萌
Owner JINAN UNIVERSITY
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