Solid support method for biological material and produced multifunctional nano-cage type bioreactor

Abstract

The invention relates to a multifunctional nanometer cage bioreactor with high stability and recoverability and a preparation method for biomaterial solid borne. The method comprises the following steps: using silicon as a raw material, a surface active agent as a template agent, trialkoxysilane with a functional group which can be loaded to the material surface as a material group reagent, and a mesoporous material synthesizing surface group as a cage body; filling a biological material as a catalytic center in the cage by an adsorption mode; using the reagent with two or more than two reactive groups as the cross-linking agent to connect an aperture modifying materials with bioactivity; and closing the catalytic center in the cage to prevent the catalytic center from escaping from a vector material and to execute a plurality of other biological functions. The multifunctional nanometer cage bioreactor synthesized according to the method is characterized by having a structure which uses a shell as the mesoporous material to fixedly load two or more than two biological materials in different types in sequence according to a selective regulation, and having the advantages of multiple functions, high stability, reutilization and the like.

Description

technical field [0001] The invention belongs to the fields of inorganic chemistry, physical chemistry and biochemistry, and particularly relates to a recyclable multifunctional nano-cage bioreactor with high stability and a preparation method for immobilizing biological materials, which is a fish-in-net immobilized enzyme method extension and expansion. Background technique [0002] Biomaterials, especially biocatalysts, such as enzymes, are increasingly used in industrial production due to their high catalytic efficiency, specific reaction, and mild reaction conditions. For example, the textile industry has adopted biological catalase for bleaching The excess hydrogen peroxide in the process is decomposed to prevent excessive oxidation of the fiber and the oxidation of the dye in the next dyeing step; in the food industry, lysozyme is used to prevent the product from being contaminated by microorganisms to ensure food safety and extend its shelf life; lactase application I...

AI Technical Summary

Problems solved by technology

Among them, covalent linkage often changes the conformation of biological materials, especially proteins, and then affects their catalytic activity; the stability range of purely physical adsorption materials is relatively narrow, and biological materials escape when the pH and ionic strength of the solution are changed. Contamination of the solution; sol-gel embedding technology can be applied to organisms of various scales, and the organisms are not easy to escape, but at the same time, it has the disadvantages of difficult mass transfer and difficult control of the caliber when the caliber is small and the substrate is large; The use of semi-permeable membranes and microcapsules also has the problem of substrate mass transfer; although bio-nanoreactors synthesized by a new fish-in-net method can immobilize biological materials with large molecular scales, such as cells and bacteria, etc. , and for biomaterials whose molecular size is smaller than the pore size of the immobilized material, it is easy to escape from the immobilized material. If aggregates are formed and then immobilized, it will easily lead to the problem of excessive aggregation of biocatalytic centers and loss of activity.

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