Preparation of molecular imprinting polymer and method for separating clenobuterol hydrochloride by using the same

A technology of clenbuterol hydrochloride and molecular imprinting, which is applied in the field of chemical engineering, can solve the problems of template residue and cross-infection, and achieve the effects of high capacity, improved sensitivity and good specificity

Inactive Publication Date: 2009-05-20
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method also has the problem of template residue, and it will ca

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of Molecularly Imprinted Polymers

[0034]Step 1: Mix 0.5 mol of the template molecule clenbuterol, the functional monomer methacrylic acid (MAA), and the crosslinking agent ethylene glycol dimethacrylate (EGDMA) in a molar ratio of 1:2:15, Dissolve it in 6.5mL of porogen (chloroform: acetonitrile = 3:10), put it into an ampoule, add 0.021g of initiator azobisisobutyronitrile (AIBN), ultrasonicate for 10min, then fill with nitrogen for 5min, vacuum Sealed, 60 ° C water bath for 24 hours, obtained clenbuterol molecularly imprinted polymer;

[0035] Step 2: Take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 38 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) were extracted by Soxhlet three times each to remove most of the template molecules, each time for 6 hours, and finally with Pure methanol...

Embodiment 2

[0042] 1. Preparation of Molecularly Imprinted Polymers

[0043] Step 1: Mix 0.5 mol of the template molecule clenbuterol, the functional monomer methacrylic acid (MAA), and the crosslinking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:3:15, Dissolve in 5mL of porogen (acetonitrile: chloroform = 10:3), put it into an ampoule, then add 0.021g of initiator azobisisobutyronitrile (AIBN), sonicate for 15min, then fill with nitrogen for 5min, and vacuum seal , in a 60°C water bath for 24 hours to obtain the clenbuterol molecularly imprinted polymer;

[0044] Step 2: Take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 45 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) were extracted by Soxhlet four times to remove most of the template molecules, each time for 5 hours, and finally with P...

Embodiment 3

[0051] 1. Preparation of Molecularly Imprinted Polymers

[0052] Step 1, mix 0.5 mol of clenbuterol template, functional monomer methacrylic acid (MAA), and crosslinking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:6:15, and dissolve Put it into 8mL porogen (acetonitrile: chloroform = 10:3), put it into an ampoule bottle, then add 0.021g initiator azobisisobutyronitrile (AIBN), sonicate for 10min, then fill it with nitrogen gas for 5min, and vacuum seal it. Water bath at 60°C for 24 hours to obtain clenbuterol molecularly imprinted polymer;

[0053] Step 2, take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 54 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) were extracted by Soxhlet 6 times each to remove most of the template molecules, 4 hours each time, and finally used Pure me...

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PUM

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Abstract

The invention relates to the preparation of molecular imprinted polymer in the technical field of chemical engineering and a method for separating clenbuterol hydrochloride with the molecular imprinted polymer. In the preparation method, clenbuterol, methacrylic acid, ethylene dimethacrylate are mixed according to the mol ratio of 1: 2-6: 15, dissolved in a pore-foaming agent, placed in an ampoule, added with an initiator, processed by ultrasonic wave, fed in nitrogen, sealed in vacuum, and then carried out thermal-initiated polymerization so as to obtain bulk polymer; and the bulk polymer is taken out, crushed, sieved and separated; after the polymer is dried, soxhlet extraction, high-temperature processing and solvent elution are carried out to get rid of a template. In the separation method, the molecular imprinted polymer homogenate is filled in a small column of polypropylene solid phase extraction, and the upper and lower ends of the filling material are respectively added with a sieve plate of polyethylene and the filling material is pressed tightly; and the small column of solid phase extraction is activated, leached and eluted so as to collect clenbuterol hydrochloride. The molecular imprinted polymer prepared by the method has high selectivity and specificity, can resist to unfriendly environment and combine with ELISA in use.

Description

technical field [0001] The invention relates to a method in the technical field of chemical engineering, in particular to a method for preparing a molecularly imprinted polymer and using it to separate clenbuterol hydrochloride. Background technique [0002] Clenbuterol hydrochloride (CLB for short) is a synthetic beta 2 -Adrenergic receptor agonists, also known as "Clenbuterol", "Cathantine", etc., are generally used in clinical practice as their hydrochloride. because of its β 2 - High receptor selectivity, strong effect, less side effects, early used in the prevention and treatment of human and animal bronchial asthma and convulsions, is an ideal anti-asthma drug, the most widely used clinically. Because clenbuterol hydrochloride can realize the redistribution of animal nutrition, improve carcass quality, and lead to an increase in lean meat rate. In 1984, clenbuterol hydrochloride was used as a feed additive for the first time abroad in the production of sheep, broile...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F2/00C08J9/26C08L35/02B01J20/26B01J20/30C07C211/52C07C209/86
Inventor 张大兵郑素连武爱波
Owner SHANGHAI JIAO TONG UNIV
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