Sustained-release preparation containing chemotherapy synergist for treating solid tumors
A sustained-release agent and synergist technology, applied in the field of anti-solid tumor sustained-release agents, can solve problems such as increased tolerance, treatment failure, and limited effective drug diffusion
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Embodiment 1
[0149] Put 90 mg of polylactic acid (PLGA, 50:50) with a peak molecular weight of 25,000-40,000 into a container, add 100 ml of dichloromethane, dissolve and mix well, add 2 mg of rapamycin and 8 mg of methotrexate, and re-shake After homogenization, the organic solvent was removed by vacuum drying. The dried solid composition is shaped immediately, subpackaged and then sterilized by radiation to obtain the anti-cancer sustained release containing 2% rapamycin and 8% methotrexate. All are percentages by weight. The drug release time of the anti-solid tumor pharmaceutical composition in physiological saline in vitro is 15-25 days, and the drug release time in mouse subcutaneous is 25-50 days.
Embodiment 2
[0151] As described in Example 1, the difference is that the peak molecular weight of polylactic acid is 35000-60000 (PLGA, 75:25), and the anticancer active ingredient and weight percentage are one of the following:
[0152] (1) 0.01-5% rapamycin and 5-20% epothilone, epothilone B, doxorubicin, epirubicin, mitomycin C, actinomycin D or a combination of dactinomycin; or
[0153] (2) 0.01-5% of rapamycin and 5-20% of fluorouracil, 6-mercaptopurine, methotrexate, flumethhotrexate, calcium levofolinate, calcium folinate, carmofur, substitute A combination of fluoride, eufurdine, topotecan, topotecan hydrochloride, cytarabine, cyclocytidine, or hydroxyurea.
Embodiment 3
[0155] Put 80mg of polyphenylpropane (p-carboxyphenylpropane (p-CPP): sebacic acid (SA) at 20:80) copolymer into a container, add 100ml of dichloromethane, dissolve and mix well, then add 1mg of radish Pamycin and 19 mg of fluorouracil were re-shaken and spray-dried to prepare microspheres for injection containing 1% rapamycin and 19% fluorouracil. Then suspend the microspheres in physiological saline containing 15% mannitol to prepare the corresponding suspension-type sustained-release injection with a viscosity of 20cp-300cp (at 20°C-30°C). The drug release time of the slow-release injection in physiological saline in vitro is 15-25 days, and the drug release time in mice subcutaneous is about 30-40 days.
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Abstract
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