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Application of abamectin residues in abamectin fermentation medium

A technology of fermentation medium and abamectin, which is applied in the direction of fermentation, microorganism-based methods, bacteria, etc., can solve problems such as environmental pollution, waste of resources, choking hair, etc., to reduce production costs, increase potency, and solve pollution problem effect

Inactive Publication Date: 2009-09-09
迈德金生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Abamectin residue contains a small amount of Abamectin (0.1-0.2%), it has been banned by the state because it can cause side effects such as diarrhea and hair choking in animals when it is used for animal deworming
Most of the manufacturers incinerate or directly discharge it into the environment, causing serious environmental pollution and waste of resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture Embodiment 1

[0015] Manufacturing Example 1: Treating 100 kg of abamectin residue to prepare the liquid nutrient components of the abamectin fermentation medium:

[0016] Strain preparation: Saccharomyces cerevisiaeMcycn cx Hanscn CGMCC AS 2.611, Bacillus subtilis (Ehrcnbcrg) Cohn AS 1.398;

[0017] Seed preparation: Weigh 50 grams of glucose, 25 grams of peptone, 10 grams of yeast extract, 25 grams of ammonium sulfate, 12 grams of sodium chloride, add water to a constant volume of 5L, and sterilize at 121°C for 20 minutes. After cooling, insert the above-mentioned strains ,Incubate at 35-37°C for 36 hours to obtain seed liquid;

[0018] Fermentation: Put 100 kilograms of abamectin residue into the fermentation device, add 3 kilograms of glucose, add 400 kilograms of water, the above seed liquid, stir evenly, control the temperature at 35-37 ℃, cultivate for 36 hours and terminate the fermentation to obtain liquid avimectin Nutrient component products of mycin fermentation medium. Its total ni...

manufacture Embodiment 2

[0019] Manufacturing Example 2: Treating 100 kg of abamectin residue to prepare paste nutrient components of abamectin fermentation medium:

[0020] Strain preparation: Saccharomyces cerevisiaeMcycn cx Hanscn CGMCC AS 2.174, Bacillus subtilis (Ehrcnbcrg) Cohn AS 1.892; Strain preparation: Weigh 100 grams of molasses, 25 grams of beef extract, 25 grams of sodium nitrate Gram, 12 grams of sodium chloride, add water to make the volume 10L, the preparation method is the same as in Example 1.

[0021] Fermentation: Put 100 kg of abamectin residue into the fermentation device, add 8 kg of molasses, 900 kg of water, and the above seed liquid, stir evenly, control the temperature at 25-30°C, aerate and stir, culture for 72 hours to terminate the fermentation, and solidify the fermentation liquid The liquid is separated, and the liquid phase is vacuum concentrated to obtain the nutrient component product of the paste abamectin fermentation medium. Its total nitrogen is 6%, and its moisture...

manufacture Embodiment 3

[0022] Manufacturing Example 3: Treating 100 kg of abamectin residue to prepare powdery nutrients for the abamectin fermentation medium:

[0023] Strain preparation: The strain is Candidautilis (henneberg) Lodder Kreger-van Rij CGMCC 2.1011; seed preparation: 20L of 12Brix wort, sterilized at 115°C for 15 minutes, after cooling, insert yeast Strains are cultivated at 30-32°C for 40 hours to obtain seed liquid;

[0024] Fermentation: Put 100 kilograms of abamectin residue into the fermentation device, add 5 kilograms of brown sugar, 200 kilograms of water, and 20L of seed liquid, stir evenly, control the temperature at 30-32°C, aerate and stir, culture for 72 hours and terminate the fermentation. The powdered abamectin fermentation medium nutrient product is obtained by drying the material. Its total nitrogen is 7.3%, and its moisture content is 5%.

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Abstract

The invention relates to the field of regeneration cycle utilization of antibiotic zymogen residues, provides a method using the abamectin residues as a raw material to prepare a product which is used for abamectin fermentation as a nutrient ingredient of a medium through processes of separation and concentration drying after microbes are fermented, and applies the product in the abamectin fermentation medium. The invention solves the problem of abamectin fermentation waste residues on environmental pollution, realizes regeneration cycle utilization of the waste residues, and achieves the aim of clean production.

Description

Technical field: [0001] The invention relates to the regeneration and recycling of antibiotic fermentation bacteria residue. In particular, it relates to the use of avermectin fermentation slag for avermectin fermentation as the nutrient component of the medium. Specifically, the abamectin residue is subjected to microbial fermentation treatment, and then added to the abamectin fermentation medium as a nutrient component of the abamectin fermentation. Background technique: [0002] Abamectin is an agricultural antibiotic produced by Streptomyces avermitilis through liquid submerged fermentation. It is mainly used to drive and kill parasites in and out of animals and agricultural pests. Because of its broad-spectrum, high-efficiency and safe anti-insect activity, it has become the most demanded agricultural antibiotic. In the process of avermectin fermentation production, Streptomyces avermitilis will multiply and produce a large amount of mycelium. After the abamectin in the myce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/08C12N1/16C12N1/20C12R1/465C12R1/865C12R1/72C12R1/125
CPCY02P20/582
Inventor 马兰孙玉胜孙淑荣
Owner 迈德金生物技术(北京)有限公司
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