Method for preparing microbial oil and fat

A technology of microbial oil and microbial bacteria, applied in the production of fat, fat oil/fat production, fermentation, etc., can solve the problems of small batch batch, long extraction time, long process route, etc., to reduce labor consumption and power consumption, The effect of reducing steaming and preheating and improving the quality of crude oil

Inactive Publication Date: 2012-01-11
BEIJING UNIV OF CHEM TECH
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Problems solved by technology

[0002] As the technology of microbial fermentation to produce oil is becoming more and more mature, the preparation of oil by microorganisms has the characteristics of rapidity, mildness, and high oil content. In the laboratory stage, the process route is cumbersome, the oil yield is low, and the production cost is high, which has become a bottleneck restricting its industrialization process
[0003] At present, there are many methods of microbial oil extraction in the laboratory stage, such as Soxhlet extraction, organic solvent extraction, acid heat method and high-pressure homogenization method, etc., but most of them are subject to small processing capacity, high cost, secondary pollution, etc. Restricted by factors, industrial application is difficult
[0004] The Chinese patent application with the application number 200810138499 discloses a "method for separation and extraction of microbial oil", which is characterized in that the high-pressure homogenization method is used to break the cells. It achieves the purpose of completely crushing cells; but its disadvantages are small processing capacity, high processing pressure, low primary crushing rate, serious emulsification when the solvent extracts oil from the water phase, and low oil yield, etc.
[0005] The Chinese patent application number 00116051 discloses "a preparation method of microbial oil", which is characterized in that oil is separated by steaming, pressing, and leaching. The advantages of this process are small powder, high production efficiency, and easy processing Large quantity; but it also has the following disadvantages: high residual oil rate, long extraction time, long-term high-temperature steaming and frying can easily cause excessive denaturation of protein, resulting in low quality of product bacteria meal, long process route, etc.
[0006] The Chinese patent with application number 031396305 discloses a "extraction method of supercritical carbon dioxide extraction of microbial oil", which is characterized in that supercritical carbon dioxide is used to extract oil, and the cells are broken by multiple pressure mutations during the extraction process, and the obtained oil is of good quality. No solvent residue; but its disadvantages are harsh process conditions, high equipment requirements, small batch capacity, and high production costs

Method used

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  • Method for preparing microbial oil and fat
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 5m after fermentation of Rhodotorula oleaginousis 3 Fermented liquid is processed according to the inventive method, and steps are as follows successively:

[0050] (1) Flocculation, add pre-prepared 0.2wt‰ polyacrylamide aqueous solution to the fermentation broth in an amount of 40ppm, the flocculation reaction time is 10min, the precipitation time is 30min, the water removal rate reaches 67%, and the dry cell content is 3wt % increased to 9 wt%.

[0051] (2) Pressure filtration, the flocculation thallus is sent to the belt press filter, and under 2.5 atmospheres, through gravity concentration dehydration, wedge-shaped pre-press dehydration and roller press dehydration for 20min, the water content of the filter cake (taken as dry thallus) is obtained. The weight is on a dry basis) is 73 wt%. The dry cell content is 27wt%.

[0052] (3) Conditioning: In the chain-type flat plate dryer, feed 120°C steam for drying and conditioning until the water content is reduced to ...

Embodiment 2

[0060] 10m fermented to Rhodotorula oleaginum 3 Fermented liquid is processed according to the inventive method, and steps are as follows successively:

[0061] (1) Flocculation, add a pre-prepared 0.5wt‰ polyacrylamide aqueous solution to the fermentation broth in an amount of 120ppm, the flocculation reaction time is 20min, the precipitation time is 1h, the water removal rate reaches 70%, and the dry cell content is 3wt % increased to 10 wt%.

[0062] (2) Pressure filtration, the flocculation thallus is sent to the belt press filter, and under 2 atmospheric pressures, through gravity concentration dehydration, wedge-shaped pre-press dehydration and roller press dehydration for 30min, the water content of the filter cake (taken as dry thallus) is obtained. The weight is based on dry basis) is 70wt%, and the content of dry cells is 30wt%.

[0063] (3) Conditioning, in the chain-type flat plate dryer, feed 125 DEG C of steam, carry out drying and tempering, the water content ...

Embodiment 3

[0070] 5 m after cultivation of Chlorella oleaginum 3 Fermented liquid is processed according to the inventive method, and steps are as follows successively:

[0071] (1) Flocculation, add pre-prepared 0.3wt‰ chitosan aqueous solution to the fermented liquid in an amount of 20ppm, stir at a low speed, the flocculation reaction time is 30min, and the settling time is 1.5h. The water rate reaches 96%, and the dry cell content of the algae increases from 0.3wt% to 7.5wt%.

[0072] (2) Pressure filtration, the flocculated thallus is sent to a belt press filter, and under 3 atmospheric pressures, through gravity concentration dehydration, wedge-shaped pre-press dehydration and roller press dehydration for 30min, the water content of the filter cake (taken as dry thallus) is obtained. The weight is based on dry basis) is 65wt%, and the content of dry cells is 35wt%.

[0073] (3) Conditioning, in the chain-type flat plate dryer, feed 125 DEG C of steam, carry out drying and temperi...

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Abstract

The invention discloses a method for preparing microbial oil and fat, which comprises the following steps: (1) collecting microbial thalli; (2) performing filter pressing on the thalli obtained in thestep (1) to obtain wet bacterial mud; (3) performing thermal refining, namely drying the wet bacterial mud obtained in the step (2) to remove most of free moisture and partial bound water in cells, and adjusting the temperature and the moisture content of the obtained dried thalli; (4) performing extrusion swelling, namely putting the dried thalli obtained in the step (3) into an extrusion swelling machine for extrusion and swelling; and (5) extracting and soaking the oil-bearing material dried thalli obtained in the step (4) to obtain mixed oil and soaked thalli residues. The method has theadvantages of simple process, large handling capacity, low energy consumption and high product quality, and belongs to the field of oil and fat preparation.

Description

technical field [0001] The invention relates to a method for preparing microbial oil, in particular to a method for preparing microbial oil by puffing and leaching. Background technique [0002] As the technology of microbial fermentation to produce oil is becoming more and more mature, the preparation of oil by microorganisms has the characteristics of rapidity, mildness, and high oil content. In the laboratory stage, the process route is cumbersome, the oil yield is low, and the production cost is high, which has become a bottleneck restricting its industrialization process. [0003] At present, there are many methods of microbial oil extraction in the laboratory stage, such as Soxhlet extraction, organic solvent extraction, acid heat method and high-pressure homogenization method, etc., but most of them are subject to small processing capacity, high cost, secondary pollution, etc. Due to the limitation of factors, industrial application is difficult. [0004] The Chines...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/10C11B1/06C12P7/64
Inventor 张栩高斌薛飞燕谭天伟
Owner BEIJING UNIV OF CHEM TECH
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