Application of midkine protein and medical device containing protein

Abstract

The invention relates to application of midkine protein and a medical device containing the protein in the technical field of biology. The invention discloses an application of protein shown as (a) or (b) for preparing a medicament for promoting the growth of cartilage tissues or a medicament for treating cartilage tissue diseases, an application of the protein for promoting cartilage tissue cell growth factors, and a medical device containing the protein; wherein the (a) is the protein of which amino acid sequence is expressed as SEQ ID No.1; the (b) is the protein which has at least 60 percent of homology of the amino acid sequence in the (a). The invention is fully different from the prior art on the cognition for MK proteins; experiments show that the MK has good proliferation effect on three kinds of cartilage cells in vivo so that the MK can be used for preparing the medicament for treating the cartilage diseases and provides a new selection for clinically treating the cartilage diseases in future, and simultaneously the MK also can be used for in vitro amplification of the cartilage cells in the cartilage tissue engineering.

Description

technical field [0001] The invention relates to the use of a protein in the field of biotechnology and a medical device containing the protein, in particular to the use of a midkine protein and a medical device containing the protein. Background technique [0002] Cartilage is an avascular connective tissue found throughout the body. It is composed of chondrocytes and extracellular matrix, which is secreted and maintained by chondrocytes. According to morphological criteria and the content of collagen and elastin, cartilage tissue can be divided into three types: hyaline cartilage, elastic cartilage and fibrocartilage. Hyaline cartilage such as nasal septum, articular cartilage, trachea and bronchi; elastic cartilage such as ears and epiglottis; fibrocartilage such as menisci and intervertebral discs. Naumann, A etc. published the title "Immunochemical and mechanical characterization of cartilage subtypes in rabbit" (immunochemical and mechanical characterization of cartil...

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Problems solved by technology

However, the study also pointed out that not all transfected cell lines with high expression of MK can produce cartilage matrix; when ATDC5 cells were cultured, adding MK protein could not promote the synthesis of cartilage matrix

Dreyfus, J etc. published a paper titled "HB-GAM / pleiotrophin but not RIHB / midkine enhances chondrogenesis in micromass" (pleiotropic factor rather than midkine promotes chondrogenesis in microsphere cultures), reviewed in the article, Dreyfus et al. found that exogenous addition of MK protein did not promote cartilage formation in micromass cultures of mesenchymal cells derived from chicken embryos Matrix synthesis

These results suggest that exogenous MK fails to promote chondrogenic differentiation of cells with chondrogenic potential in culture

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