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Silicon oxide and protamine microcapsule and preparation method thereof

A protamine and silicon oxide technology, applied in biochemical equipment and methods, microsphere preparation, microcapsule preparations, etc., can solve the problems of difficult control of the physical and chemical properties of microcapsules, and achieve controllable size and stability of microcapsules. Good, reproducible results

Inactive Publication Date: 2010-04-14
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively difficult to control the physical and chemical properties of microcapsules

Method used

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  • Silicon oxide and protamine microcapsule and preparation method thereof
  • Silicon oxide and protamine microcapsule and preparation method thereof
  • Silicon oxide and protamine microcapsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The polystyrene sodium sulfonate (PSS) of 50mg is dissolved in the calcium chloride solution (concentration is 0.33mol / l) after the filter of 15ml, adds the sodium carbonate solution of the 0.33mol / l after the filter of 15ml to this solution, in React in an electromagnetic stirrer with a rotation speed of 1000rpm for 20s, turn off the electromagnetic stirrer and let it stand for 15 minutes, then turn on the electromagnetic stirrer, stir the reaction solution evenly, take 5ml and add it to a centrifuge tube, and take 4 tubes in total. The four-tube suspension was centrifuged in a centrifuge at a rotational speed of 3000rpm for 3 minutes, then washed with deionized water, and the centrifugation-washing operation was repeated twice.

[0025] Configure 0.03mol / l sodium silicate solution, adjust its pH to 8 with hydrochloric acid; configure 2mg / ml protamine solution, add 5844mg sodium chloride to it and adjust its pH to 6.5-7.0 with hydrochloric acid and sodium hydroxide bet...

Embodiment 2

[0030] The polystyrene sodium sulfonate (PSS) of 50mg is dissolved in the calcium chloride solution (concentration is 0.33mol / l) after the filter of 15ml, adds the sodium carbonate solution of the 0.33mol / l after the filter of 15ml to this solution, in React in an electromagnetic stirrer with a rotation speed of 1000rpm for 20s, turn off the electromagnetic stirrer and let stand for 15 minutes. Finally, turn on the electromagnetic stirring, stir the reaction solution evenly, take 5ml and put it into a centrifuge tube, and take 4 tubes in total. The four-tube suspension was centrifuged in a centrifuge at a rotational speed of 3000rpm for 3 minutes, then washed with deionized water, and the centrifugation-washing operation was repeated twice.

[0031] Configure 0.03mol / sodium silicate solution, adjust its pH to 8 with hydrochloric acid; configure 2mg / ml protamine solution, add 5844mg sodium chloride to it and adjust its pH to 6.5-7.0 with hydrochloric acid and sodium hydroxide ...

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Abstract

The invention relates to a silicon oxide and protamine microcapsule and a preparation method thereof. The silicon oxide and protamine microcapsule is prepared by using a calcium carbonate particulate as a template and sodium silicate and protamine as raw materials, wherein the mass ration of the sodium silicate to the protamine is 1:1-7:1. The preparation method comprises the following steps: firstly preparing the calcium carbonate particulate with negative charges as the template; interacting the protamine with positive charges and the calcium carbonate particulate with negative charges and alternately absorbing to the template; inducing the forming of silicon oxide by using the biomineralizing function of the protamine when three to five double-layers are generated on the template; removing the inner core of the calcium carbonate by EDTA-2Na. The invention is gentle in reaction conditions during the preparation of the silicon oxide and protamine microcapsule. The prepared silicon oxide and protamine microcapsule has the advantages of gentle reaction condition, size controllability, good stability, and excellent repeatability.

Description

technical field [0001] The invention relates to a silicon oxide and protamine microcapsule and a preparation method thereof, in particular to a preparation method for preparing microcapsules by hybridizing silicon oxide and protamine. Background technique [0002] Nowadays, microcapsules are increasingly used in microreactors, drug carriers, encapsulation protection of cells and enzymes, and gene transfection. Traditional microcapsule preparation methods mainly contain three kinds: (1) chemical method, chemical method refers to the method that chemical reaction takes place in the liquid phase to form capsules. There are mainly two kinds of chemical methods currently used, namely interfacial polycondensation method and radiation chemical method. Although such methods can obtain microcapsules with relatively uniform size, their size is often difficult to adjust. (2) Physicochemical method, this method forms capsules in the liquid phase, that is, in the mixture of the capsule...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/00A61K47/42A61K47/04C12N11/00C12N15/88B01J13/02
Inventor 姜忠义张蕾孙倩芸
Owner TIANJIN UNIV
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