Application of amniotic epithelial cells and cell preparations thereof

A technology for amniotic epithelial cells and cell preparations, applied in the field of biomedicine, can solve problems such as the problem of neurogenic bladder treatment, and achieve the effect of restoring physiological functions and having a wide range of sources.

Inactive Publication Date: 2010-06-09
SHANGHAI ICELL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide new therapeutic drugs or methods for the existing neurogenic bladder treatment problems

Method used

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  • Application of amniotic epithelial cells and cell preparations thereof
  • Application of amniotic epithelial cells and cell preparations thereof
  • Application of amniotic epithelial cells and cell preparations thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. hAEC isolation and culture

[0053] Take the placenta of healthy caesarean section pregnant women, and strip the amniotic membrane from the chorion. Rinse with PBS to remove blood cells, digest with 0.25% trypsin at 37°C for 30 min, pipette, add RPMI1640 containing 10% FBS to stop the digestion, filter through a 100-mesh sieve, centrifuge at 1,500 rpm, discard the supernatant, and dilute the mixture with 1×10 4 / cm 2 Inoculate in 10cm dish. hAECs were placed in RPMI-1640 (10% FBS, 100 μg / ml streptomycin, 100 U / ml penicillin, 10 ng / ml EGF and 0.3 mg / ml glutamine) at 37°C, 5% CO 2 cultured in a cell culture incubator.

Embodiment 2

[0054] Example 2. Preparation of hAEC cell preparation

[0055] Example 1 hAEC cultured for 24-72 hours, remove the medium, digest the adherent hAEC with 0.25% trypsin and centrifuge, then rinse with 2ml of PBS solution and centrifuge again, resuspend in PBS and adjust the cell concentration to 105 / μl for later use, namely hAECs cell preparations were obtained.

Embodiment 3

[0056] Example 3.hAECs Treat Neurogenic Bladder Animals

[0057] 1. Animals

[0058] SPF grade female adult Sprague-Dawley rats were used in the experiment, purchased from Shanghai Slack Experimental Animal Center, weighing 250-300g, reared in a single cage, free to ingest water and food, and lighted to form a day / night cycle of 12-12h. Experimental animals were divided into control group, operation (spinal cord injury) group and treatment (cell transplantation) group.

[0059] 2. Surgical method

[0060] The animals in the operation group were anesthetized by intraperitoneal injection of sterile 10% chloral hydrate (400mg / kg), lying prone, making an incision about 2 cm long centered on the thoracic 12 spine, cutting the skin, and bluntly peeling off the fascia layer by layer, Muscles, after the spinous process and lamina are exposed, the thoracic 11 spinous process and lamina are bitten off, and the spinal cord and central vein are exposed. The exposed spinal cord was full...

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Abstract

The invention relates to the technical field of biomedicine, in particular to application of amniotic epithelial cells and cell preparations thereof. The invention discloses application of the amniotic epithelial cells in preparation of a medicament for treating neurogenic bladder and cell preparations of the medicament for treating spinal neurogenic bladder. The amniotic epithelial cells or the cell preparations containing the amniotic epithelial cells can effectively treat or improve the neurogenic bladder, particularly spastic neurogenic bladder, and improve or recover the physiological function of the bladder; meanwhile, the amniotic epithelial cells have wide sources, so that the phenomenon of ethic or morality is avoided. Therefore, the amniotic epithelial cells and the cell preparations provide a new effective choice for clinical treatment of the neurogenic bladder in the future.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of amnion epithelial cells and their cell preparations. Background technique [0002] Normal urine control and excretion is the result of a complex interplay of smooth muscles, skeletal muscles, brain control, and the autonomic nervous system. [0003] Bladder and urethral dysfunction caused by damage to the central nervous system or peripheral nerves that regulate and control the physiological activities of urination is called neurogenic bladder (Neurogenic Bladder). [0004] In fact, almost any neurological disorder can affect bladder function. The nervous system that controls bladder function includes the central and peripheral nerve tissues, so as long as a part of the nerve tissue is damaged, it may affect the bladder function, stroke, Parkinson's disease, multiple sclerosis, diabetes, spinal cord Bloating, spinal cord or head injury, pelvic trauma or s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61K47/02A61K47/34A61K47/42A61K47/46A61P13/00A61P25/00
Inventor 郭礼和刘新庞力
Owner SHANGHAI ICELL BIOTECH
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