Method of producing propionic acid and propionate by microorganism fermentation

Abstract

The invention relates to a method of producing propionic acid and propionate by microorganism fermentation, which is characterized by using microorganism fermentation technology to produce propionic acid; with propionibacterium, charging the nitrogen into basic fermentation culture medium which takes glycerin as a carbon source for anaerobic fermentation culture; movably adding the aqueous solution of calcium hydroxide, or the alkaline solution or the saline solution of sodium hydroxide and calcium carbonate with the ongoing of fermentation, adjusting the fermentation liquor and maintaining the pH value between 6.5 to 7.5; movably adding the aqueous solution of glycerin after the fermentation preformed for a period of time; the fermentation is finished when the content of glycerin stops increasing and is more than 35g / L; and then filtering the fermentation liquor through a pressure filter, getting the filtered solution, charging the filtered solution into a spray tower for spray drying, and the dried powder is the propionate product. The invention has the effects: the method can substitute the existing chemical synthesis to produce propionic acid, remarkably improving the safety of the propionic acid product, and is a green food additive; moreover, the problem of consumption of non-renewable resources (such as oil) can be solved without a secondary processing, the negative impact generated by chemical synthesis can be prevented, and the process flow and the cost can be saved; and the invention particularly opens up an industrialization stage of the propionic acid production by fermentation and the simultaneous production of the propionate.

Description

technical field [0001] The invention relates to microbial fermentation technology. Propionic acid is fermented and then reacted with salt or alkali to form propionate such as calcium propionate. The whole process is completed once in a fermenter. Background technique [0002] Propionic acid is an important raw material for feed, medicine, food industry, etc. Propionic acid and its salts such as calcium propionate, zinc propionate, and potassium propionate can effectively inhibit mold and aerobic bacillus, and are basically harmless to the human body. They are widely used in grains, feed and food, and are good antiseptic and fresh-keeping products. agent. In addition, propionic acid can also be used as electroplating aids, emulsifiers and the production of biodegradable plastics. With the development of agriculture, textile, food, medicine and other industries, the demand for pesticide herbicides, paint fungicides, food protection agents and feed additives is increasing, an...

AI Technical Summary

Problems solved by technology

In addition, acrylonitrile method, ethanol carbonyl method, n-propanol oxidation method, propane (butane, paraffin) liquid phase oxidation method, etc. can also be used in the production of propionic acid, but due to the large investment in equipment and other reasons, it has not been used in practice so far.

[0004] The main disadvantages of producing propionic acid by chemical synthesis are: (1) large consumption of non-renewable resources (such as petroleum); (2) as a food additive, it has a relatively large potential safety hazard; (3) heavy pollution, high cost and poor operating conditions harsh

[0005] Domestic and foreign studies on the production of propionic acid by microbial fermentation mostly focus on using glucose, whey, lactose, and lactic acid as carbon sources, using free cell fermentation technology or cell immobilized fermentation technology, and using propionic acid bacteria or mixed strains to ferment and produce propionic acid , but the production of propionic acid is low, mostly in the stage of laboratory research