Biological tissue material for artificial organs and preparation method thereof
A biological tissue and artificial organ technology, which is applied in the field of biological tissue materials for human soft tissue grafts and human organs to replace artificial biological tissue materials, achieves the effects of simple preparation process, low technical requirements and good elasticity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] The thoracic aorta blood vessels of live slaughtered pigs were taken out from the slaughterhouse, stored in iced saline, and quickly transferred to the laboratory. Remove adipose tissue and reticular connective tissue attached to the surface. Rinse repeatedly with a large amount of normal saline to remove blood and fat until no blood seeps out. Afterwards, use an aqueous solution of benzalkonium bromide with a mass concentration of about 0.03% to sterilize and degrease for about 45 minutes, and then repeatedly rinse with phosphate buffer to remove benzalkonium bromide. The benzalkonium bromide aqueous solution is prepared by adding clear water to a commercially available benzalkonium bromide solution with a mass concentration of 5%. Phosphate buffer consists of 0.14M NaCl, 0.003M KCl, 0.01M Na 2 HPO 4 and 0.0016M of KH 2 PO 4 preparation.
[0027] The rinsed porcine aorta blood vessels and the cross-linking solution were placed in a shaker for cross-linking under ...
Embodiment 2
[0031] The preparation process of the biotissue material for artificial organs with porcine aortic vessels as the substrate and oxidized sodium alginate as the cross-linking agent prepared in this example is basically the same as in Example 1, except that the porcine aortic vessels are killed alive. The mass concentration of the benzalkonium bromide aqueous solution used for disinfection and degreasing is about 0.05%, the pH value of the cross-linking system is about 4.0, the pH value of the cross-linking solution is about 4.0, and the pH value of the cross-linking solution is about 4.0. The concentration of oxidized sodium alginate is about 10g of oxidized sodium alginate in 100ml cross-linking solution; the cross-linking time is 3 days. Preparation of buffer: first prepare 0.1M citric acid solution, then prepare 0.1M sodium citrate solution, take 1 part of sodium citrate solution and add about 2.1 parts of citric acid solution to prepare citric acid / sodium citrate buffer solu...
Embodiment 3
[0034] The preparation process of the biotissue material for artificial organs with porcine aortic vessels as the substrate and oxidized sodium alginate as the cross-linking agent prepared in this example is basically the same as in Example 1, except that the porcine aortic vessels are killed alive. For the pig's descending aorta, the mass concentration of benzalkonium bromide aqueous solution used for disinfection and fat removal is about 0.04%, the pH value of the cross-linking system is about 7.4, and the pH value of the cross-linking solution is about 7.4. The concentration of oxidized sodium alginate is about 15g of oxidized sodium alginate in 100ml cross-linking solution; the cross-linking time is 3 days. Buffer preparation: prepare 0.14M NaCl, 0.003M KCl, 0.01M NaCl in the same double distilled water 2 HPO 4 and 0.0016M of KH 2 PO 4 , and then adjust the pH to 7.4 with HCl. The amount of cross-linking solution is, per 1cm 2 The tissue was treated with 1ml-5ml cross...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More