Method for detecting bifidobacterium in sour milk

A detection method and technology for bifidobacteria, which are applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of long detection time, interference of bifidobacteria detection, and difficulty in detection, etc. Achieve the effect of reducing detection cost, reducing identification steps, and improving detection efficiency
CN101851664AInactive Publication Date: 2010-10-06INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
Publication Date
2010-10-06
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention relates to a method for detecting bifidobacterium in sour milk, which comprises the following steps of: preparing diluent, culture medium and antibiotic solution; treating and culturing a sample; counting a bacterial colony; and performing microscopic examination, Gram staining and catalase test. The antibiotic solution contains neomycin sulfate and paromomycin sulfate. The method of the invention can effectively eliminate the interference of other lactic acid bacteria, accurately detect the live bacteria number of the bifidobacterium in the sour milk containing 4 kinds of lactic acid bacteria, reduce the detection cost and improve the detection efficiency.
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Description

technical field

[0001] The invention relates to a method for detecting bifidobacteria in yoghurt, more particularly to a method for detecting bifidobacteria in yoghurt containing four kinds of lactic acid bacteria. The invention belongs to the detection technical field of microbial products. Background technique

[0002] Bifidobacteria are the first physiological bacteria discovered by humans, and they are probiotics that can colonize the intestinal tract of healthy people. The bacteria in the intestinal tract of breastfed infants account for more than 92% of the total flora, and it has been confirmed that the quantity and quality of bifidobacteria are one of the important signs of human health. Studies at home and abroad have reported that bifidobacteria have a variety of physiologically active functions and can be used as one of the sources of host nitrogen. At the same time, they can synthesize vitamins, laxatives, enhance the body's immune function, reduce cholesterol a...

Claims

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