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Method for detecting bifidobacterium in sour milk

A detection method and technology for bifidobacteria, which are applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of long detection time, interference of bifidobacteria detection, and difficulty in detection, etc. Achieve the effect of reducing detection cost, reducing identification steps, and improving detection efficiency

Inactive Publication Date: 2010-10-06
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, people have long neglected the need for a method to accurately detect the viable count of Bifidobacteria in yogurt, resulting in very little bifidobacteria in many fermented milk products on the market and difficult to detect
At the same time, lactic acid bacteria that are beneficial to health, such as Lactobacillus acidophilus and Lactobacillus casei, are increasingly added to fermented milk, together with Streptococcus thermophilic lactis and Lactobacillus bulgaricus in the starter used in yogurt processing, The presence of these lactic acid bacteria interferes with the detection of bifidobacteria
[0004] In 2004, the country issued the "GB / T 4789.34-2008 Detection Method for Bifidobacteria in Food". The medium used in this method is non-selective medium counting, which can realize the detection of viable Bifidobacteria, but for yogurt products In other words, other lactic acid bacteria in yogurt can grow on this medium, and each inspection requires multi-step identification to obtain accurate data, which takes a long time and costs high

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  • Method for detecting bifidobacterium in sour milk
  • Method for detecting bifidobacterium in sour milk
  • Method for detecting bifidobacterium in sour milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Sample to be tested: the number of living bacteria of Bifidobacterium lactis known to be 1.0×10 5 Yogurt sample in cfu / mL

[0045] Preparation of samples: Add bifidobacterium lactis powder (product of Co. Hansen Co., Ltd.) with known number of live bacteria to yogurt products in a certain proportion, so that the number of live bacteria of Lactobacillus acidophilus and Bifidobacterium lactis in yogurt up to 1.0×10 5 , the total number of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus reached 1.0×10 6 .

[0046] Instruments and equipment: ultra-clean workbench (Sujing Group Antai Company, BCM-1000A), autoclave (Shanghai Shen'an Medical Device Factory, LDZX-50KB), anaerobic tank (Changsha Yuyi Electronic Technology Co., Ltd., 2.5L), electric thermostatic incubator (Huangshi Hengfeng Medical Instrument Co., Ltd., SKP02.600), biological microscope (Chongqing Optical Instrument Factory, XSZ-4).

[0047]Reagent: 0.9% sterile saline.

[0048] MR...

Embodiment 2

[0104] Sample to be tested: the number of viable Bifidobacterium longum is known to be 1.0×10 6 Yogurt sample in cfu / mL

[0105] Preparation of samples: Add Bifidobacterium longum powder (preserved strain of Mengniu Dairy (Beijing) Co., Ltd., CGMCC No.2265) with known viable count to yogurt products in a certain proportion to make acidophilic milk in yogurt The number of live bacteria of Bacillus and Bifidobacterium longum reached 1.0×10 6 cfu / mL, the number of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus reached 1.0 × 10 6 cfu / mL.

[0106] Reagent: 0.9% sterile saline.

[0107] MRS medium (g / L): peptone 10.0, beef powder 5.0, yeast powder 4.0, glucose 20.0, Tween 801.0ml, dipotassium hydrogen phosphate (7H 2 O) 2.0, sodium acetate (3H 2 O) 5.0, triammonium citrate 2.0, magnesium sulfate (7H 2 O) 0.2, manganese sulfate (4H 2 O) 0.05, agar 15.0. Dissolve the above ingredients in distilled water, adjust the pH to 6.2±0.1, and dilute to 1000m...

Embodiment 3

[0163] Sample to be tested: the known viable count of Bifidobacterium adolescentis 1.0×10 6 Yogurt sample in cfu / mL

[0164] Sample preparation: Add Bifidobacterium adolescentis powder (preserved strain of Mengniu Dairy (Beijing) Co., Ltd., CGMCC No.2264) with known number of viable bacteria to yogurt products in a certain proportion to make acidophilic milk in yogurt The number of live bacteria of Bacillus and Bifidobacterium adolescent reached 1.0×10 6 cfu / mL, the number of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus reached 1.0 × 10 6 cfu / mL.

[0165] Reagent: 0.9% sterile saline.

[0166] MRS medium (g / L): peptone 10.0, beef powder 5.0, yeast powder 4.0, glucose 20.0, Tween 801.0ml, dipotassium hydrogen phosphate (7H 2 O) 2.0, sodium acetate (3H 2 O) 5.0, triammonium citrate 2.0, magnesium sulfate (7H 2 O) 0.2, manganese sulfate (4H 2 O) 0.05, agar 15.0. Dissolve the above ingredients in distilled water, adjust the pH to 6.2±0.1, and ...

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Abstract

The invention relates to a method for detecting bifidobacterium in sour milk, which comprises the following steps of: preparing diluent, culture medium and antibiotic solution; treating and culturing a sample; counting a bacterial colony; and performing microscopic examination, Gram staining and catalase test. The antibiotic solution contains neomycin sulfate and paromomycin sulfate. The method of the invention can effectively eliminate the interference of other lactic acid bacteria, accurately detect the live bacteria number of the bifidobacterium in the sour milk containing 4 kinds of lactic acid bacteria, reduce the detection cost and improve the detection efficiency.

Description

technical field [0001] The invention relates to a method for detecting bifidobacteria in yoghurt, more particularly to a method for detecting bifidobacteria in yoghurt containing four kinds of lactic acid bacteria. The invention belongs to the detection technical field of microbial products. Background technique [0002] Bifidobacteria are the first physiological bacteria discovered by humans, and they are probiotics that can colonize the intestinal tract of healthy people. The bacteria in the intestinal tract of breastfed infants account for more than 92% of the total flora, and it has been confirmed that the quantity and quality of bifidobacteria are one of the important signs of human health. Studies at home and abroad have reported that bifidobacteria have a variety of physiologically active functions and can be used as one of the sources of host nitrogen. At the same time, they can synthesize vitamins, laxatives, enhance the body's immune function, reduce cholesterol a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/30C12Q1/06C12Q1/04C12R1/01
Inventor 刘爱萍蒋菁莉牛天娇代青梅赵红峰
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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