Complex bacterial preparation and application thereof

A technology of composite microbial agent and viable count, which is applied in the field of agricultural microorganisms, can solve the problems of low degradation ability, limited ability to deal with animal excrement, limitations, etc., and achieve the effect of promoting growth, good effect and low cost

Inactive Publication Date: 2010-10-20
江苏加德绿色能源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial population needs to be added to the microbial culture system in batches at 15°C, and to the aqueous matrix containing excreta in a semi-continuous or continuou

Method used

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  • Complex bacterial preparation and application thereof
  • Complex bacterial preparation and application thereof
  • Complex bacterial preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The preparation of embodiment 1 microbial fermented liquid

[0036]This embodiment only takes Escherichia coli (ATCC10795), Trichoderma (ATCC10045), Pseudomonas fluorescens (ATCC11150), and Bacillus subtilis (ATCC10774) as examples to describe the specific content of the invention in detail, and does not limit the composite microorganism of the present invention Selection of four sources of microorganisms in inoculum.

[0037] (1) Activate the above four kinds of microorganisms into slant medium for preservation respectively, and culture them at 25-37°C for 24-48 hours. The slant culture and composition of 4 kinds of microorganisms are as follows:

[0038] Escherichia coli and Pseudomonas fluorescens slant medium adopt LB medium, and the liquid medium is composed of: 10 grams of peptone, 5 grams of yeast extract, 10 grams of sodium chloride, 1000 milliliters of tap water, 15 grams of agar, pH=7.2;

[0039] Trichoderma species slant medium adopts PDA medium, and the me...

Embodiment 2

[0050] Embodiment 2: Utilize compound microbial bacterial agent to process cow dung anaerobic fermentation to produce biogas

[0051] (1) Four kinds of microbial fermented liquids prepared in embodiment 1 are mixed uniformly by the following weight parts to become composite microbial inoculum: 1 part of escherichia coli liquid, 2 parts of Trichoderma liquids, 2 parts of Pseudomonas fluorescens liquids, 4 parts of Bacillus subtilis share.

[0052] (2) Get 600 kilograms of fresh cow dung from the dairy farm, add water to prepare a mixture with a water content of about 95%, divide it into two equal parts according to the mass, and add it to two identical fermentation tanks (with a volume of 1000 liters).

[0053] (3) Add a methanogenic inoculum (selected from cesspit sediment) whose dry weight accounts for 10% (g / g) of the mixture of cow dung and water, and adjust the pH to 6.8-7.2.

[0054] (4) In one of the anaerobic fermentation tanks, add the composite bacterial agent prepar...

Embodiment 3

[0057] Example 3: Biogas production by anaerobic fermentation of straw by using compound microbial agent

[0058] (1) Four kinds of microbial fermented liquids prepared by embodiment 1 are mixed uniformly to become composite microbial inoculum by following parts by weight: 2 parts of escherichia coli liquid, 2 parts of Trichoderma liquids, 2 parts of Pseudomonas fluorescens liquids, 5 parts of Bacillus subtilis share.

[0059] (2) Choose 400kg of rice straws that are not polluted by toxic substances, and cut them short manually or mechanically, with a length of less than 3cm.

[0060] (3) Add water equivalent to 2 times the mass of the straw to the crushed straw to moisten it, stir it well overnight to make the straw swell.

[0061] (4) Add quicklime (calcium oxide) with 1% mass of straw raw material to the swollen straw, mix evenly, and stack retting. The stack retting temperature is kept at 50° C. for 6 days.

[0062] (5) Divide the stacked retting straw into two equal par...

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Abstract

The invention belongs to the technical field of the agricultural microbiology and discloses a complex bacterial preparation and application thereof. The complex bacterial preparation is prepared by mixing the following components: 1 to 3 parts of fermentation liquor of Escherichia.coli, 2 to 3 parts of fermentation liquor of Trichoderma virens, 1 to 4 parts of fermentation liquor of Pseudomonas fluorescens and 4 to 6 parts of fermentation liquor of Bacillus subtilis. The complex bacterial preparation provided by the invention not only can improve the methane yield of straws and cow dung and improve the methane generating speed, but also can further reduce content of solid waste by 30 percent. The method has the advantages of quick response, low cost, simple using method, easy popularization and the like and can be popularized and used.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a composite microbial agent and its application. Background technique [0002] Straw biomass is crop residues with a high content of cellulose components, including straws of corn, rice, wheat, cotton, etc. Straw, as a kind of natural cellulose, can be biodegraded and utilized, and can adapt to the development of products that meet environmental requirements. Its chemical composition mainly contains cellulose, hemicellulose and lignin. For decades, people have made great progress in theoretical research and practical application of cellulose and related enzymes, but so far there is no microorganism or a set of enzymes that can degrade cellulose on a large scale according to traditional methods, and have achieved remarkable results. Economic benefit, its utilization rate is only about 1% at present, the main reasons for limiting its utilization are the high cos...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/14C12P5/02C02F11/04C12R1/19C12R1/885C12R1/39C12R1/125
CPCY02E50/343Y02E50/30
Inventor 周楚新支银芳孙珊
Owner 江苏加德绿色能源有限公司
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