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Inner-wall waveguide mode capillary fiber based online evanescent field biosensor

A biochemical sensor and capillary technology, applied in the direction of optical waveguide light guide, optical waveguide coupling, cladding fiber, etc., can solve the constraints of the integration, miniaturization and stabilization of the optical fiber biochemical sensing system, and affect the stability and service life of the probe. , the limited load of the sensor probe, etc., to achieve the effect of high signal stability, improved mechanical strength, and improved sensitivity

Inactive Publication Date: 2010-12-01
HARBIN ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still many key problems to be solved urgently: (1) The load capacity of the sensor probe based on the traditional optical fiber structure is limited, and it is difficult to improve the sensitivity; (2) Most of the detection is in an open environment, and the probe is easily eluted due to diffusion. Affect the stability and service life of the probe; (3) The sensing area is easily disturbed by ambient light
The above problems restrict the final development of the integration, miniaturization and stabilization of the optical fiber biochemical sensing system

Method used

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  • Inner-wall waveguide mode capillary fiber based online evanescent field biosensor
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  • Inner-wall waveguide mode capillary fiber based online evanescent field biosensor

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1: Inner wall waveguide capillary fiber optic cyanide sensor based on CdSe quantum dot modification

[0040] Fully mix a certain amount of tetraethyl orthosilicate, absolute ethanol, and KH-560 in a certain proportion in a 25ml round-bottom beaker, and heat the mixed solution in a water bath at a temperature of 60°C. During the heating process, add 0.1 mol / L dilute hydrochloric acid solution dropwise to adjust the pH to 3, then install a condenser tube and start stirring on a magnetic stirrer. After about 40 minutes, the mixed solution began to form a uniform phase. At this time, TOPO-CdSe quantum dots were added, and the stirring was continued. With the extension of the reaction time, the state changed from the original dilute solution to a viscous liquid. After reacting for 1 hour, the sol was removed from the capillary fiber to form a sensitive layer 2 .

[0041] The detection of cyanide will be based on the anti-quenching principle. Aspirate the capillary ...

Embodiment 2

[0042] Example 2: Inner wall waveguide capillary fiber optic DNA sensor based on biomolecular modification

[0043] First use HNO 3 The surface of the ring waveguide 3 is activated, and then the inner surface of the optical fiber is silanized with 3-aminopropyltriethoxysilane (APTMS), so that the surface has amino groups. Adding a molecule capable of linking two positively charged molecules such as glutaraldehyde, adding Streptavidin. Finally, add biotin (Biotin) oligo-stranded DNA molecules such as 5'-biotin-CAGCGAGGTGAAAACGACAAAAGGGG solution. Finally, the DNA hybridization experiment is carried out, and the single-stranded gene fragment labeled with the fluorophore Cy3 passes through the sensing optical fiber fixed with a sensitive membrane. If the DNA fragment structure is: 5′-Cy3-CCCCTTTTGTCGTTTTCACCTCGCTG, the structure is captured by the base pairing reaction. The excitation light excites Cy3 to generate fluorescence through the evanescent field, and the signal is als...

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Abstract

The invention provides an inner-wall waveguide mode capillary fiber based online evanescent field biosensor. The fiber has a microchannel structure, wherein a micro-pipe structure enclosing the channel is an annular waveguide layer; the inner wall surface of the annular waveguide layer is a sensitive layer, while the outer layer is a barrier layer; the outer layer of the barrier layer is a coating layer, and a protective layer is arranged outside the coating layer; two segments of groove structures are arranged at the lateral side of the fiber, and an incident fiber and an emergent fiber are coupled at the position of the groove structures respectively; one end of the capillary fiber is used as a sample inlet, while the other end is used as a sample outlet; the sample outlet is connected with a vacuum pump; a light source is arranged at the sample inlet; a grating filter or a long pass filter is connected in series with the emergent fiber, which is connected with a spectrograph; and the spectrograph is connected with a computer. The biosensor can be used in various fields, such as environment detection, food production line detection, medication and the like.

Description

technical field [0001] The invention relates to an online optical fiber sensor, in particular to an online evanescent wave biochemical sensor based on an inner wall waveguide type capillary optical fiber structure. Background technique [0002] Fiber optic biochemical sensors are a branch of the family of fiber optic sensors. Since 1980, Peterson (J.I.Peterson, S.R.Goldstein, R.V.Fitzgerald, D.K.Buckhold, Fiber optic pH probe for physiological use, Anal.Chem., 1980, 52(6): 864-869) and others introduced fiber optic technology into the field of biochemical sensing , realized the design of the optical fiber pH sensor and applied it to physiological detection, the optical fiber biochemical sensor has received extensive attention. Due to the advantages of optical fiber sensing, such as strong anti-electromagnetic interference, high sensitivity, good electrical insulation, safety and reliability, corrosion resistance, and miniaturization, optical fiber biochemical sensors are wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/64G02B6/24G02B6/02
Inventor 苑立波杨兴华张涛
Owner HARBIN ENG UNIV
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