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Method for preparing biologic herbicide-containing fertilizer from biogas slurry

A technology of biological fertilizer and biogas slurry is applied in the field of comprehensive utilization of agricultural waste, which can solve the problems of narrow disease prevention range, low disease prevention effect, unstable application effect, etc., and achieves easy operation, good synergy and effect. stable effect

Active Publication Date: 2011-04-06
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to: in view of the current mass construction of biogas projects and the continuous increase of biogas slurry production, biogas slurry, as a nutrient-rich biomedical fertilizer, has unstable application effects in agricultural production, low effect on disease prevention and control. In view of the narrow scope of disease, it is proposed to prepare biomedical fertilizer with enhanced disease prevention function by adding compound and broad-spectrum antagonistic spore bacteria

Method used

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  • Method for preparing biologic herbicide-containing fertilizer from biogas slurry
  • Method for preparing biologic herbicide-containing fertilizer from biogas slurry

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The acquisition and strain characteristics of three spore-forming bacterial strains:

[0029] The biogas slurry was obtained from the biogas project of Nanjing Shantian Dairy Farm. Collect 8-10 ml of biogas slurry in a 25 ml test tube, heat the test tube in a water bath at 80°C for 15 minutes, take it out and cool it to room temperature, and draw 200 microliters of biogas slurry into a 9 cm diameter petri dish under sterile conditions Then quickly add 20ml of PDA medium at 55-60°C, mix the two thoroughly and place it until the medium is completely solidified, then add 15ml of PDA medium at 55-60°C to the culture dish, gently horizontally After shaking well, place it horizontally, wait until the medium is completely solidified, evenly inoculate 5 pieces of mycelium with a diameter of 7 mm and fresh fungus of strawberry wilt on the medium, and place the culture dish at 28±2°C for 4 to 5 days. Use the inoculation loop to pick the colony that produces the inhibition zone 2...

Embodiment 2

[0034] Preparation of Three Bacillus Culture Medium

[0035] Preparation of test tube slant medium: Weigh 3 grams of beef extract, 10 grams of peptone, and 5 grams of sodium chloride, dissolve them in 1000 ml of water, adjust the pH to 7.4-7.6, add 15-20 grams of agar, heat and boil until the agar dissolves, Make up the volume to 1000ml, cool down properly and put it into test tubes, the filling volume is 1 / 5~1 / 6 of the test tube height, plug the test tube mouth with a test tube stopper, autoclave at 121°C for 25 minutes, take it out while it is hot, and put it on an inclined plane Until it is cooled for later use, the height of the inclined plane shall not exceed 1 / 3 of the height of the test tube.

[0036] Preparation of liquid seed medium: 3 grams of beef extract, 10 peptones, and 5 grams of sodium chloride, dissolved in 1000 ml of water, adjusted to pH 7.4 to 7.6, and then divided into 80 / 250 ml triangular flasks with cotton Stopper the mouth of the bottle, autoclave at 1...

Embodiment 3

[0040] Preparation of compound microbial agent

[0041] The preserved bacterial classification of three kinds of bacillus bacterium in embodiment 1 is inoculated respectively in the test tube slant medium and carries out the test tube slant surface activation, then, the bacterial classification activated is inoculated to the 250ml triangle that 80ml liquid seed medium is housed with the inoculating loop In the bottle, the Erlenmeyer flask was placed in a shaker for 24 hours, the rotating speed of the shaker was 160r / min, and the culture temperature was 28±2°C; after the end, insert the 800ml liquid production culture with 5% inoculum size (V / V). In a 2000ml Erlenmeyer flask, place the Erlenmeyer flask on a shaking table for cultivation, the rotating speed of the shaking table is 160r / min, and the cultivation temperature is 28±2°C. After 3 to 4 days of cultivation, the microscopic inspection of the spore formation rate is above 95%. Finish the cultivation; spray the liquid cult...

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Abstract

The invention relates to a method for preparing a biologic herbicide-containing fertilizer from biogas slurry by using biogas slurry as a main raw material. The method is characterized by comprising the following steps of: screening biogas slurry with a sieve of 120 meshes, and adding a compound microorganism bactericide prepared from antagonistic bacilli to prepare the biologic herbicide-containing fertilizer. In the biologic herbicide-containing fertilizer, the number of antagonistic bacilli reaches 5-6*10<7> CFU / ml. The antagonistic bacilli comprise geobacilli, bacillus amyloliquefaciens and bacillus subtilis.

Description

technical field [0001] The invention relates to a method for preparing biological medicine fertilizer from biogas slurry. It belongs to the field of comprehensive utilization of agricultural waste. Background technique [0002] According to statistics, by the end of 2005, there were 17 million biogas digesters in my country, with an annual output of 6.5 billion m3 of biogas. 3 , by 2010, the total number of large and medium-sized biogas projects in large-scale farms across the country has reached about 4,700, and as of 2008, the number of large-scale farms in Jiangsu Province alone has reached 140,000, including more than 4,700 large-scale farms. There are more than 1,000 places in the project, and the pool capacity is 100m 3 There are more than 300 projects above, and 80% of them are built in pig farms. Livestock and poultry manure is converted into energy through biogas projects and at the same time produces a large volume of biogas slurry. The safe, reasonable and effi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
CPCY02E50/30Y02W30/40
Inventor 马艳常志州徐跃定张建英
Owner JIANGSU ACAD OF AGRI SCI
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