Method for domesticating saline land by microalgae

A technology of saline-alkali land and microalgae, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc., and reduce treatment costs Cost, Fertility increase, Effect of increasing microalgae biomass

Inactive Publication Date: 2013-01-16
北京芳能科技有限公司
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Problems solved by technology

) Among the existing main methods for controlling saline-alkali land, the water washing and salt discharge adopted by the physical method are likely to cause the loss of soil nutrients, and require large material and financial resources, while the fertilization or acid-base balance adopted by the chemical method is likely to cause soil compaction and Excessive governance costs
Although physical and chemical methods can temporarily change the salinity of the soil, they cannot essentially change the soil fertility of the original saline-alkali land.

Method used

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  • Method for domesticating saline land by microalgae
  • Method for domesticating saline land by microalgae
  • Method for domesticating saline land by microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Use Cylindrosporium to treat the saline-alkali land around Canglang Canal in Binhai New Area, Tianjin. The soil in this area contains about 0.2% nitrogen, 0.071% phosphorus, 1.5% salt, and 0.6% potassium. , for mild saline-alkali land, the better treatment time is from March to June in spring.

[0039] Step 1. Laboratory algae cultivation

[0040] Prepare the basal medium for cultivating Cylindrospora, the formula of FN30 medium is:

[0041] Ammonium nitrate 0.5-4g / L Sodium chloride 0.25-2.0g / L

[0042] Potassium dihydrogen phosphate 0.01-0.09g / L Magnesium sulfate 0.01-0.05g / L

[0043] Calcium chloride 0.01-0.05g / L Sodium acetate 0.005-0.03g / L

[0044] Urea 0.02-0.08g / L Trace elements 0.5-2ml / L

[0045] Wherein the trace element preparation method is:

[0046] Ferric chloride 4-7mg Sodium molybdate 0.1-0.4mg

[0047] Manganese Chloride 1-5mg Disodium EDTA 40-95mg

[0048] Zinc sulfate 0.3-1.5mg Cobalt chloride 0.02-0.2mg

[0049] Add distilled water to make up t...

Embodiment 2

[0076] Use Nostoc algae in the saline-alkali area around Yantai, Shandong. The soil nitrogen content in this area is about 0.17%, phosphorus content is about 0.073%, salt content is about 2.3%, and potassium content is about 0.52%. It is a relatively moderate saline-alkali land , the better processing time is from March to June in spring.

[0077] Step 1. Laboratory algae cultivation

[0078] Prepare the basal medium for cultivating Nostoc, the formula of FN31 medium is:

[0079] Potassium nitrate 0.05-0.5g / L Calcium chloride 0.003-0.03g / L

[0080] Dipotassium hydrogen phosphate 0.01-0.8g / L Sodium chloride 0.25-2.0g / L

[0081] Potassium dihydrogen phosphate 0.07-0.3g / L Ferric chloride 0.001-0.01g / L

[0082] Magnesium Sulfate 0.02-0.06g / L Naphthaleneacetic Acid 0.2-0.6mg / L

[0083] Trace elements 0.5-2ml / L

[0084] Wherein the trace element preparation method is:

[0085] Boric acid 150-500mg Copper sulfate 2-10mg

[0086] Manganese chloride 60-200mg Ammonium molybdate 1...

Embodiment 3

[0113] Spirulina is used to treat the saline-alkali land around the farm in Taiping Town, Binhai New Area, Tianjin. The soil in this area contains about 0.13% nitrogen, 0.069% phosphorus, 4.4% salt, and 0.75% potassium. , for relatively severe saline-alkali land, the better treatment time is from March to June in spring.

[0114] Step 1. Laboratory algae cultivation

[0115] The basal medium for preparing cultured spirulina, the formula of FN32 medium is:

[0116] Sodium bicarbonate 5-35g / L Sodium chloride 0.5-3.0g / L

[0117] Sodium carbonate 1-9g / L Magnesium sulfate 0.05-0.2g / L

[0118] Dipotassium hydrogen phosphate 0.1-1.8g / L Calcium chloride 0.01-0.09g / L

[0119] Sodium nitrate 0.8-4.0g / L Gibberellin 0.05-0.4mg / L

[0120] Trace elements 0.5-2ml / L

[0121] Wherein the trace element preparation method is:

[0122] Boric acid 1-5mg Ferric chloride 150-800mg

[0123] Zinc sulfate 0.04-0.3mg Sodium molybdate 5-40mg

[0124] Manganese sulfate 0.35-2.5mg Cobalt chloride 3...

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Abstract

The invention discloses a method for domesticating cylindrospermopsis raciborskii, nostoc, spirulina or microcolus and application thereof. The method comprises the following steps of: preparing a culture medium; carrying out domestication of salt tolerance, domestication of reduction of the content of nitrogen and phosphorus, domestication of adaption to the quality of water to be treated and the like on screened algae; carrying out step-by-step amplification cultivation; inducting the algae into an alkaline land so that microalgae reproduces massively by utilizing photosynthesis and absorbsand fixes nitrogen and phosphorus in the atmosphere and water source; and after reproduction, draining microalgae cultivating water so that the microalgae is deposited in the soil. The microalgae contain a large number of elements, such as nitrogen, phosphorus and the like. After the soil is treated once by the method, the content of nitrogen is increased by 18 to 23 percent, the content of phosphorus is increased by 11 to 15 percent and the total salt content is reduced by 12 to 30 percent in the soil so that not only loss of nutrient elements caused by a washing method is avoided, but also hardening of the soil and secondary pollution to the water source and the environment which are caused by application of a fertilizer are avoided. After the soil is treated repeatedly by the method, the fertility of the soil is gradually improved. Moreover, the method can accelerate recovery of the soil so as to finally fulfill the aim of improving the ecological environment.

Description

technical field [0001] The invention provides a method for improving saline-alkali land through microalgae, thereby improving soil fertility of saline-alkali land; in particular, it relates to a method for improving saline-alkali land with nitrogen-fixing cyanobacteria. Background technique [0002] Saline-alkali lands are widely distributed around the world, covering more than 30 countries on six continents. It is mainly distributed in India, Pakistan, Russia, South Africa and other countries, with a total area of ​​about 956 million hectares. Among them, India and Pakistan have 8.09 million hectares, and Russia has 7.5 million hectares. The area of ​​saline-alkali land in the western United States accounts for about half of the irrigated area, and the output of saline-alkali is reduced by more than 25% every year. Land salinization is an important part of land desertification, which seriously threatens the local agricultural and animal husbandry production, the prosperit...

Claims

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Application Information

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IPC IPC(8): C12N1/12C09K17/00C12R1/89C09K109/00
Inventor 单东杰李涛
Owner 北京芳能科技有限公司
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