Culture method for efficiently inducing accumulation of nannochloropsis oculata fat
A technique of Nannochloropsis and fat is applied in the culture field of efficiently inducing fat accumulation in Nannochloropsis, which can solve the problems of less research on Nannochloropsis, and achieve the effect of accelerating division and improving fat accumulation.
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Embodiment 1
[0049] 1. The formula of FN17 medium is as follows:
[0050] Sodium chloride 35g / L Sodium nitrate 160mg / L
[0051] Ferric chloride 4.5mg / L Disodium edetate 2.5mg / L
[0052] Sodium dihydrogen phosphate 12.0mg / L Trace elements 1ml / L
[0053] The preparation method of trace elements is as follows: take 11 mg of copper sulfate, 25 mg of zinc sulfate, 22.5 mg of cobalt chloride, and 220 mg of manganese chloride and add distilled water to 1 L.
[0054] Solution A:
[0055] Sodium Nitrate 290g / L Trace Elements 1ml / L
[0056] Wherein the trace element formula is:
[0057] Copper sulfate 1.5g / L Zinc sulfate 4.5g / L
[0058] Cobalt chloride 3g / L Manganese chloride 35g / L
[0059] Add water to make up to 1L
[0060] Solution B:
[0061] Sodium dihydrogen phosphate 190g / L
[0062] Solution C:
[0063] Ferric chloride 7g / L
[0064] Solutions A:B:C were mixed according to the ratio of 18:1:18, and the daily addition amounts were 6ml / L, 8ml / L and 10ml / L respectively. Dissolve the a...
Embodiment 2
[0093] 1. Multi-conditional induction of ultrasound and culture conditions
[0094] In a 300L small-scale photoreactor system, add FN17 medium, inoculate Nannochloropsis-X57 with a medium volume ratio of 1:8, and the inoculation concentration is 2.1×10 5 cells / ml, the culture temperature is 26-28°C, the light is 15000-18000 lux, the light time is 14 hours / day, and 0.8ml / L of the mixed solution of solution A, B and C is added every day (solution A: B: C according to 18:1:18 ratio), cultured for 8 days, with a frequency of 18kHz, a power of 4w, a treatment time of 15s, ultrasonic treatment twice, each time interval of 10s, and then flow rate of 1.0-1.5L / min into the concentration of 5% CO 2 Add the mixed solution of solution A and B every day (solution A: B is mixed according to the ratio of 15: 1), the addition amount is 30 μl / L, the amount of solution C added at one time is 1.2ml / L, after induction culture for 2 days, then With a frequency of 10kHz, a power of 3w, a treatmen...
Embodiment 3
[0101] In a 300L small photoreactor system, add FN17 medium, inoculate Nannochloropsis-X57 with medium volume ratio of 1:8, and the inoculation concentration is 2.21×10 5 cells / ml, the culture temperature is 26-28°C, the light is 15000-18000 lux, the light time is 14 hours / day, and 0.8ml / L of the mixed solution of solution A, B and C is added every day (solution A: B: C according to 18:1:18 ratio), cultured for 7 days, with 35kHz frequency, 6w power, treatment time 60s, sonication once, and then 2% CO at a flow rate of 0.5-1.0L / min 2 , add the mixed solution of solution A and B every day (solution A: B is mixed according to the ratio of 10:1), the amount added is 100 μl / L, the amount of solution C added at one time is 0.5ml / L, after 2 days of induction culture, 20kHz The frequency, the power of 5w, the treatment time of 15s, ultrasonic treatment once, induced fat accumulation for 4 days.
[0102] The results show that Nannochloropsis-X57 grows rapidly with this method, and en...
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