Method for extracting and separating statins substance

A statin and substance technology, applied in the field of using supercritical CO2 extraction technology to extract and separate statin substances, can solve the problems of organic solvent residue, high production cost, affecting the quality of finished products, etc., to prevent the existence of human harmful substances and prevent oxidation And the effect of escape, improve production efficiency

Active Publication Date: 2011-06-08
NEW FOUNDER HLDG DEV LLC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the separation and extraction process of statins synthesized by microorganisms mainly adopts the organic solvent extraction method, which has the following disadvantages: 1, a large amount of dichloromethane, acetonitrile, acetone, ethyl acetate, and acetic acid are used in the separation and extraction process. organic solvents such as butyl ester, which will produce a large amount of waste water, waste gas and other toxic and harmful substances in the production process, which will pollute the environment and be harmful to the health of operators; 2. The process route is relatively complicated, and the extraction process has many steps. The technical requirements of the process point are many; 3. The loss of the target product is large and the yield is low, so the production cost is high; 4. The purity of the finished product is low; 5. The finished product has organic solvent residues, which affects the quality of the finished product

Method used

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  • Method for extracting and separating statins substance
  • Method for extracting and separating statins substance
  • Method for extracting and separating statins substance

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1 Supercritical CO 2 Selection of process parameters in extraction

[0041] In the method of the present invention, the separation step, the type of entrainer, extraction temperature, extraction pressure, extraction time, CO 2 The flow rate, the volume-to-weight ratio of entrainer to mycelial powder all have important effects on the recovery rate. In the present invention, the inventors have done a lot of creative research work to select the above parameters.

[0042] 1. Selection of separation step conditions

[0043] in supercritical CO 2 The separation step after extraction can adopt a primary separation process or a secondary separation process, wherein the conditions of the separation step have an important impact on the recovery rate. The conditions in the process of secondary separation are investigated below, and the investigation process is as follows:

[0044] Method: The mycelia obtained after fermentation of Penicillium citrinum HD-32-2 (i.e. th...

Embodiment 2

[0111] With the mycelia obtained after the fermentation of Penicillium citrinum HD-32-2 (i.e. the crude product of mevastatin, purchased from Chongqing Industrial Microbiology Pharmaceutical Engineering Technology Research Center) as raw material, its water content was determined to be 20%, and it was pulverized into 40 mesh fine powder. Put the above fine powder into supercritical CO 2 In the extraction kettle, adjust the extraction temperature, pressure, time, control CO 2 Flow rate for extraction, wherein: the temperature of the extraction kettle is 55°C, the pressure is 30.0MPa, the time is 3h, CO 2 The flow rate is 20L / h. Add entrainer ethanol (entrainer: the volume-to-weight ratio of fine powder is 3: 1 (L: Kg) in the extraction tank, and its flow rate is set at 2L / h. Then the first separating tank separation temperature is set to 35 ℃, separates The pressure is set to 6MPa to carry out the first separation. Afterwards, the separation temperature of the second separat...

Embodiment 3

[0113] The mycelium obtained after fermentation of Aspergillus terreus CA951 (ie the crude product of lovastatin, purchased from Chongqing Industrial Microbiology Pharmaceutical Engineering Technology Research Center) was used as raw material, and the method described in Example 2 was used for extraction and separation. The recovery of lovastatin was determined to be 92% by HPLC.

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Abstract

The invention relates to a method for extracting and separating statins substances by utilizing a supercritical CO2 extraction process. The method comprises the steps of extracting and separating, wherein in the extracting step, an entrainer is one or more of ethanol, acetone, ethyl acetate and butyl acetate. The method provided by the invention is suitable for extracting and separating the statins substances, such as mevastatin, pravastatin or lovastatin and the like. By adopting the method, the usage of organic solvents can be greatly reduced, the production cost is lowered, the environmental pollution is reduced, and the quality and the recovery rate of a finished product are improved, therefore, the statins substances are prepared economically and cleanly.

Description

technical field [0001] The present invention relates to a method for extracting and separating statins, more specifically, the present invention relates to a method using supercritical CO 2 Extraction technology to extract the method of separating statins. Background technique [0002] Statins are cholesterol-lowering drugs that reduce the amount of cholesterol produced by the liver. It is clinically used to reduce serum total cholesterol, low density lipoprotein (Low Density Lipoprotein, LDL), very low density lipoprotein and triglyceride, and can increase high density lipoprotein. [0003] Statins are hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, which block intracellular hydroxymetholone by competitively inhibiting endogenous cholesterol synthesis rate-limiting enzyme HMG-CoA reductase The acid metabolism pathway reduces intracellular cholesterol synthesis, thereby feedback stimulating the number and activity of low-density lipoprotein receptors on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D309/30
CPCY02P20/54
Inventor 岳光
Owner NEW FOUNDER HLDG DEV LLC
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