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Preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation

A technology for protein separation and magnetic microspheres, which is applied in the fields of peptide preparation, microsphere preparation, chemical instruments and methods, etc. It can solve the problem of slow magnetic response speed, low magnetic content of microspheres, inability to meet the requirements of biological separation and purification efficiency, high Sensitivity, high throughput and other issues, to achieve the effects of size controllable, uniform particle size, and fast magnetic response

Active Publication Date: 2011-08-17
SUZHOU WEIDU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The magnetic content of microspheres synthesized by this method is very low, and the magnetic response speed is very slow, which cannot meet the practical application.
[0009] The existing magnetic microsphere preparation technology cannot simultaneously achieve the requirements of fast magnetic response and controllable monodisperse particle size (0.5-50um), and cannot meet the requirements of biological separation and detection for purification efficiency, high sensitivity, and high throughput

Method used

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  • Preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation
  • Preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation
  • Preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation

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Experimental program
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Effect test

Embodiment 1

[0027] 1) Preparation of Fe 3 o 4 Magnetic nanoparticles and surface modification

[0028] 50g Fe was mixed with a high-speed disperser 3 o 4 The nanoparticles were dispersed in 250ml of deionized water, mixed evenly, poured into a three-necked flask, placed in a 50°C water bath, filled with nitrogen, stirred for 0.5 hours, and then 10g of ammonia water was added to the reaction system to start the reaction. After 10 minutes 0.005 g of undecylenic acid was added. After 1 hour, raise the temperature of the water bath to 65°C, open the three-necked flask and keep it warm for 1 hour, and stop the reaction after all the ammonia water evaporates. The black solid was separated by magnetic adsorption, and then washed three times with water and absolute ethanol, and then vacuum-dried at 50°C to obtain Fe 3 o 4 Powder, spare.

[0029] 2) Preparation of Fe 3 o 4 Magnetic Nanoparticle Assemblies

[0030] SDS was dissolved in deionized water to form a 0.01 wt% aqueous solution, ...

Embodiment 2

[0036] 1) Fe 3 o 4 Preparation and surface modification of magnetic nanoparticles

[0037] 50g Fe was mixed with a high-speed disperser 3 o 4 The nanoparticles were dispersed in 250ml of deionized water, mixed evenly, poured into a three-necked flask, placed in a 75°C water bath, filled with nitrogen, stirred for 0.5 hours, and then 10g of ammonia water was added to the reaction system to start the reaction. After 10 minutes 0.5 g of octadecenoic acid was added. After 1 hour, raise the temperature of the water bath to 85°C, disperse the three-necked flask and keep it warm for 1 hour, and the reaction ends after all the ammonia water evaporates. The black solid was separated by magnetic adsorption, and then washed three times with water and absolute ethanol, and then vacuum-dried at 50°C to obtain Fe 3 o 4 Magnetic nano particle powder, spare.

[0038] 2) Preparation of Fe 3 o 4 magnetic particle assembly

[0039] Dissolve PVA in deionized water to form a 0.5 wt% aque...

Embodiment 3

[0044] 1) Fe 3 o 4 Preparation and surface modification of magnetic nanoparticles

[0045] 50g Fe was mixed with a high-speed disperser 3 o 4 The nanoparticles were dispersed in 250ml of deionized water, mixed evenly, poured into a three-neck flask, placed in a 90°C water bath, filled with nitrogen, stirred for 0.5 hours, and then 10g of ammonia water was added to the reaction system to start the reaction. After 10 minutes 10 g of linolenic acid was added. After 1 hour, raise the temperature of the water bath to 95°C, open the three-necked flask and keep it warm for 1 hour, and wait for the ammonia water to evaporate completely to end the reaction. The black solid was separated by magnetic adsorption, washed with water and absolute ethanol three times respectively, and vacuum-dried at 50° C. to obtain magnetic nanoparticle powder for future use.

[0046] 2) Preparation of magnetic particle assembly

[0047] Tween 80 was dissolved in deionized water to form a 5.0 wt% aque...

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Abstract

The invention discloses a preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation, which comprises the steps of: modifying magnetic nano particles by adopting a surface chemical modifying agent to obtain an oleophylic and hydrophobic surface and dispersing into an organic solvent, preparing oil drops rich in the magnetic nano particles by using a template-process high-pressure shearing action, stabilizing the oil drops to be suspended in water by means of static endowed by a surfactant and a steric hindrance effect, gradually contracting through the organic solvent in the slowly evaporated oil drops to form a magnetic nano particle complex, wherein the magnetic nano particle complex is a base core; carrying out silicon oxide coating on the complex by adopting a sol-gel method to form a silicon oxide shell with uniform thickness and controllable size; and carrying out alcohol washing and water washing multiple times to finally obtain the monodisperse silicon oxide magnetic microspheres with controllable particle diameter and a core shell structure. The silicon oxide magnetic microspheres prepared by adopting the preparation method have the characteristics of uniform particle diameter, high magnetic response speed and the like, and are easy to realize industrialized mass production.

Description

technical field [0001] The invention relates to a method for preparing silicon oxide magnetic microspheres with controllable monodisperse particle size and core-shell structure, which are used for nucleic acid purification and protein separation. Background technique [0002] Magnetic microspheres have the characteristics of small particle size, large surface area, and magnetic properties can be modified with active groups on the surface according to different needs. They have broad application prospects in the field of biomedicine and have been widely used in the separation of biomolecules (nucleic acid, protein) Purification, immunodiagnosis and other biomedical fields. In practical applications, microspheres are generally required to have the characteristics of monodispersity, controllable particle size, and fast magnetic response. [0003] The uniformity of the shape and size of the magnetic microspheres ensures the consistency of their surface physical and chemical pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02H01F1/11C12N15/11C07K1/14
Inventor 范新文刘照关
Owner SUZHOU WEIDU BIOTECH CO LTD
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