Sclerotium rolfssii scleroglucan online fermentation extraction method and system

A technology of scleroglucan and extraction system, which is applied in the field of on-line fermentation and extraction of Sclerotin scleroglucan, can solve the problems of high cost of separation and extraction, low yield of polysaccharide extraction, clogging of ultrafiltration membranes, etc., to reduce the cost of extraction , Low equipment cost, and the effect of increasing productivity

Active Publication Date: 2011-08-17
天津实发中科百奥工业生物技术有限公司
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Problems solved by technology

However, due to the high content of Sclerotin scleroglucan, its high viscosity and high molecular weight, it is easy to be adsorbed by the resin when passing through the

Method used

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  • Sclerotium rolfssii scleroglucan online fermentation extraction method and system
  • Sclerotium rolfssii scleroglucan online fermentation extraction method and system

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Embodiment Construction

[0022] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0023] A method for on-line fermentation and extraction of Sclerotin scleroglucan, characterized in that: the steps of the method are as follows:

[0024] (1). Bacteria culture:

[0025] A 1000ml shake flask was used to cultivate the Sclerotinia seed solution in a constant temperature shaker. The components and mass percentages of the strain culture medium are: 4% sucrose, 0.3% sodium nitrate, 0.075% corn steep liquor, 0.075% yeast powder, 0.05% potassium chloride, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate heptahydrate %, the rest is water, pH: 5.0. The temperature of the shaker was 30° C., the rotation speed of the shaker was 150 rpm, and the culture period was 48 hours.

[0026] (2). Use a 30L fermenter to ferment Sc...

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Abstract

The invention relates to a Sclerotium rolfssii scleroglucan online fermentation extraction method, which comprises the following steps: (1) culturing a strain; (2) fermenting the Sclerotium rolfssii by using a fermentation tank; and (3) extracting polysaccharide liquid on line by ceramic membrane separation. The invention also relates to a Sclerotium rolfssii scleroglucan online fermentation extraction system, which comprises the fermentation tank, a feeding system, an oxygen supply system, a steam system, a control system and a ceramic membrane separation system. In the Sclerotium rolfssii scleroglucan online fermentation extraction method and the Sclerotium rolfssii scleroglucan online fermentation extraction system, a Sclerotium rolfssii glucan product is continuously extracted from fermentation liquor by ceramic membrane separation in a fermentation process, the Sclerotium rolfssii glucan does not accumulated in the fermentation liquor, and thus, the inhibition of the synthesis ofthe Sclerotium rolfssii glucan product due to problems of increases in viscosity of fermentation liquor, reduction in dissolved oxygen, reduction in pH value and the like. Meanwhile, post treatment is not required, the yield is high, the production process is simplified, the equipment cost is low, and the operation cost is low.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to an online fermentation and extraction method and system of Sclerotin scleroglucan. Background technique [0002] Scleroglucan, also known as Scleroglucan and Scleroglucan, belongs to high molecular weight β-glucan, and is a kind of neutral glucan produced by the metabolism of fungus Sclerotinia. The main chain of the molecule is composed of β-1,3-D-glucopyranose, and there is a β-1,6-glucopyranose side chain every three glucose units. The molecular structure of Sclerotin scleroglucan determines that it has the following properties: high temperature resistance (up to 120°C), shear resistance, high viscosity and is not affected by ion concentration, and has strong stability in application sex. Sclerotin scleroglucan belongs to extracellular polysaccharide, which can be dissolved in hot and cold water, and has good water binding, stability, viscosity and...

Claims

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Application Information

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IPC IPC(8): C12P19/08C12M1/36C12M1/12C12M1/00C12R1/645
CPCC12M29/02C12M29/06C12M41/26C12M41/12C12M47/10
Inventor 魏呐许勤虎王祥河张英筠吴頔吕丛潮王珺安峰
Owner 天津实发中科百奥工业生物技术有限公司
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