Preparation method of core-shell magnetic conductive polymer microspheres and application thereof

A conductive polymer and polymer technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of increasing production costs and unfriendly environment

Inactive Publication Date: 2011-09-07
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heterogeneous polymerization method can obtain high-quality core-shell magnetic composite microspheres, but cross-linking agents and / or surfactants need to be added during the preparation process; this not only increases the production cost, but is also environmentally unfriendly

Method used

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  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof
  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof
  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Core-shell Fe 3 o 4 Preparation of / PANI composite microspheres

[0056] To a 100mL three-neck flask, add 0.1g Fe 3 o 4 , 40mL 0.1mol / L hydrochloric acid, ultrasonically dispersed at room temperature for 5min, sealed and placed in a 5°C thermostat for 12h, magnetic separation to remove the hydrochloric acid aqueous solution and retain the magnetic core; then add 15mL absolute ethanol, 0.1 g re-distilled aniline, ultrasonically dispersed for 5 minutes, and placed in a 5°C thermostat for 12 hours.

[0057] Place the flask in an ultrasonic cleaner, and control the water temperature of the ultrasonic cleaner to 5-10°C. Dissolve 0.25mL of 12mol / L hydrochloric acid in 45mL of distilled water, then add 0.28g of ammonium persulfate to dissolve, slowly add the oxidant solution dropwise, and control the dropping time to 0.5h; keep stirring for 3h under the assistance of ultrasound.

[0058] Magnetic separation of solid and liquid, washing with water for several ti...

Embodiment 2

[0059] Example 2: Core-shell Fe 3 o 4 Preparation of / PPy composite microspheres

[0060] The aniline in embodiment 1 is replaced with pyrrole, and 0.28g ammonium persulfate is used 0.40g FeCl 3 ·6H 2 O is replaced, and other operations remain unchanged, and Fe can be obtained 3 o 4 / PPy samples.

Embodiment 3

[0061] Embodiment 3: utilize Fe 3 o 4 / PANI Composite Microspheres Separation and Purification of Escherichia coli (E.coli DH5α) Plasmid DNA

[0062] Take 10mg of dedoped Fe 3 o 4 / PANI magnetic balls were placed in a 1.5mL sterile centrifuge tube, ultrasonicated and sterilized twice with 1mL 70% (v / v) ethanol solution, then transferred to another sterile centrifuge tube, and 40μL E.coli DH5α was added in sequence Plasmid DNA crude extract and 1mL Tris-HCl (10mmol / L, pH 7.8) buffer, shake well, place at room temperature for 20min until the DNA is adsorbed on the surface of the magnetic ball, collect the magnetic ball under a magnetic field, and discard the supernatant. The magnetic balls were washed twice with 1 mL of 70% ethanol solution, magnetically separated, the supernatant was discarded, and the magnetic balls that adsorbed DNA were placed at room temperature to volatilize the ethanol.

[0063] Add 0.4mL of 2mol / L NaCl solution to the centrifuge tube containing the m...

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Abstract

The invention discloses a preparation method of core-shell magnetic conductive polymer microspheres. Ferrite (Fe3O4, gamma-Fe2O3, MFe2O4; M = Fe, Co, Ni, Mn and the like) nano or micro spherical or near-spherical particles are used as cores; firstly, surface hydroxyl protonation is performed on the magnetic cores to fully combine the magnetic cores before compositing with monomer molecules by the electrostatic interaction and hydrogen-bond action between the protonated hydroxy and monomer aniline, pyrrole or derivatives thereof; and in the oxidation polymerization process, the monomer molecules are controlled to form cores and grow on the surfaces of the magnetic cores, thereby preparing the core-shell magnetic conductive polymer microspheres with high magnetic responsibility and good dispersity. By the characteristics that the amido-containing magnetic polymer microspheres are positively charged in a high-pH solution while the DNA molecules in a tissue or cell lysis solution are negatively charged, the prepared magnetic composite microspheres are applied to DNA separation and purification assisted by an external magnetic field.

Description

technical field [0001] The invention relates to a preparation method of a core-shell magnetic conductive polymer microsphere and its application in DNA separation and purification, and belongs to the technical field of preparation of magnetic composite materials and biological separation materials. Background technique [0002] The separation and purification of DNA plays a very important role in the fields of genetic engineering, biology, medicine and genetics. DNA separation and purification techniques can be generally divided into two categories: without external magnetic field assistance and with external magnetic field assistance, that is, non-magnetic separation and magnetic separation. There are many methods of non-magnetic separation, which are described in many documents and patents (N. Ramasubu Ramanyan Chinese patent: DNA purification method and purified DNA.CN 1390229A, January 8, 2003), Such as: phenol-chloroform-isoamyl alcohol method, cesium chloride-ethidium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02C08G73/06C08K9/02C08K3/22C12N15/10
Inventor 盖利刚马万勇姜海辉韩小云
Owner QILU UNIV OF TECH
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