Synthetic method of L-serine

Abstract

The invention discloses a synthetic method of L-serine, which comprises the following steps: A) transforming the concentration of a substrate: adding the substrate, namely glycine and serine hydroxymethyltransferase accounting for 3%-8% by weight into a biological reactor; feeding 37% of formaldehyde water solution when the temperature is 40-50 DEG C and getting L-serine transformation liquid by transformation; B) purifying through an ultrafiltration membrane: purifying the L-serine transformation liquid in the A) step through the ultrafiltration membrane under the conditions that the temperature is 25-35 DEG C, the pressure at an inlet is 0.4-0.8MPa and the pressure at an outlet is 0.1-0.3MPa; C) performing adsorption and elution through ion-exchange resin: regulating the pH value of penetrating fluid in the B) step to 4.5-5.5, then exchanging the L-serine transformation liquid onto resin through the ion-exchange resin and further eluting with 0.8-1.5mol / L of hydrochloric acid; D) concentrating through a nanofiltration membrane: concentrating L-serine eluent in the step C) through the nanofiltration membrane under the conditions that the temperature is 25-35 DEG C, the pressure at the inlet is 0.4-0.8MPa and the pressure at the outlet is 0.1-0.3MPa; and getting an L-serine finished product after concentration. The synthetic method of the L-serine is simple in operation, short in production period, low in cost, high in yield and low in environmental protection pressure, and suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a synthesis method of amino acids, in particular to a new synthesis method of L-serine. Background technique [0002] L-Serine (L-Serine), chemically named 2-amino-3-hydroxypropionic acid, is a sugar-producing amino acid, which can be biosynthesized from glyceric acid as a precursor in the body, and threonine and glycine can also be converted into serine . Although L-serine is a non-essential amino acid, it is widely used as a biochemical reagent in food, feed, medicine, agriculture and cosmetics industries. L-serine is in the middle of amino acid metabolism and participates in the synthesis of many biological substances (such as glycine, methionine, purine, etc.), and its metabolism runs extremely fast. Therefore, compared with other types of amino acids, the production of L-serine by direct fermentation is very difficult. Due to the limitations of various technical condition...

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at technical defects such as cumbersome process, high cost and high environmental protection pressure in the existing technology of chemically synthesizing L-serine, and provides a kind of L-serine with simple process, low cost, suitable for large-scale industrial production and low environmental protection pressure synthesis method

[0009] The present invention also aims at the defect that the existing microbial fermentation method synthesizes L-serine with a long cycle and fails to meet the requirements of industrial production, and provides a short production cycle and a synthesis method for L-serine that meets the requirements of industrial production