Synthetic method of L-serine

A synthesis method and technology for serine, applied in the field of biomedicine, can solve the problems of failing to meet the requirements of industrial production, long cycle of synthesizing L-serine, short production cycle, etc., and achieve reduced product extraction difficulty, high finished product yield, and production cycle. short effect

Inactive Publication Date: 2011-10-19
HENGDIAN GRP JIAYUAN CHEM +1
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at technical defects such as cumbersome process, high cost and high environmental protection pressure in the existing technology of chemically synthesizing L-serine, and provides a kind of L-serine with simple process, low cost, suitable for large-scale industrial production and low environmental protection pressure synthesis method
[0009] The present invention also aims at the defect that the existing microbial fermentation method synthesizes L-serine with a long cycle and fails to meet the requirements of industrial production, and provides a short production cycle and a synthesis method for L-serine that meets the requirements of industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Use an electronic balance to weigh 2000g of the substrate glycine, add 100g of wet bacteria to a bioreactor equipped with 1000g of deionized water, and when the temperature is 50°C, add 37% formaldehyde aqueous solution to control the pH value to 8.5, and pass nitrogen gas Keep the oxygen partial pressure at 0.01-0.03MPa and convert it into L-serine conversion solution; transfer the converted L-serine conversion solution to the ultrafiltration membrane storage tank at a temperature of 25°C and an inlet pressure of 0.4Mpa , the outlet pressure is 0.1Mpa under the condition of 0.1Mpa and is purified by the ultrafiltration membrane of 2000 Daltons; the yield of the purified L-serine conversion liquid is 97%, and the above-mentioned permeate purified by the ultrafiltration membrane is purified with 0.8mol / L of hydrochloric acid was adjusted to a pH value of 5.5, and then the L-serine conversion solution was exchanged on the resin by an ion exchange resin, and then eluted in...

Embodiment 2

[0036] Weigh 2000g of the substrate glycine with an electronic balance, add 100g of wet bacteria into a reaction kettle equipped with 1000g of deionized water, and at a temperature of 45°C, add 37% formaldehyde solution to control the pH value to 7.5, and feed nitrogen to maintain oxygen. Under the condition of aqueous solution with a partial pressure of 0.01-0.03MPa, it is converted into L-serine conversion liquid; the above-mentioned converted L-serine conversion liquid is transferred to the ultrafiltration membrane storage tank, at a temperature of 25°C, the inlet pressure is 0.4Mpa, and the outlet pressure is 0.4Mpa. Under the condition of a pressure of 0.1Mpa, it was purified through an ultrafiltration membrane with a membrane pore size of 2000 Daltons; the yield of the purified L-serine conversion solution was 96%, and the above-mentioned permeate purified by the ultrafiltration membrane was treated with 0.8mol / L hydrochloric acid Adjust the pH value to 5.5, and then exch...

Embodiment 3

[0039] Weigh 2000g of the substrate glycine with an electronic balance, add 100g of wet bacteria into a reaction kettle equipped with 1000g of deionized water, and at a temperature of 40°C, add 37% formaldehyde solution to control the pH value to 8.0, and feed nitrogen to maintain oxygen. Under the condition of partial pressure of 0.01-0.03MPa aqueous solution, it is converted into L-serine conversion solution; the above-mentioned converted L-serine conversion solution is transferred to the ultrafiltration membrane storage tank, and at a temperature of 25 °C, the inlet pressure is 0.4Mpa, and the outlet pressure is 0.4Mpa. Under the condition of pressure of 0.1Mpa, it was purified by an ultrafiltration membrane with a membrane pore size of 2000 Daltons; the yield of the purified L-serine conversion liquid was 95%, and the above permeate purified by the ultrafiltration membrane was purified with 0.8mol / L The hydrochloric acid is adjusted to a pH value of 5.5, and then the L-seri...

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Abstract

The invention discloses a synthetic method of L-serine, which comprises the following steps: A) transforming the concentration of a substrate: adding the substrate, namely glycine and serine hydroxymethyltransferase accounting for 3%-8% by weight into a biological reactor; feeding 37% of formaldehyde water solution when the temperature is 40-50 DEG C and getting L-serine transformation liquid by transformation; B) purifying through an ultrafiltration membrane: purifying the L-serine transformation liquid in the A) step through the ultrafiltration membrane under the conditions that the temperature is 25-35 DEG C, the pressure at an inlet is 0.4-0.8MPa and the pressure at an outlet is 0.1-0.3MPa; C) performing adsorption and elution through ion-exchange resin: regulating the pH value of penetrating fluid in the B) step to 4.5-5.5, then exchanging the L-serine transformation liquid onto resin through the ion-exchange resin and further eluting with 0.8-1.5mol/L of hydrochloric acid; D) concentrating through a nanofiltration membrane: concentrating L-serine eluent in the step C) through the nanofiltration membrane under the conditions that the temperature is 25-35 DEG C, the pressure at the inlet is 0.4-0.8MPa and the pressure at the outlet is 0.1-0.3MPa; and getting an L-serine finished product after concentration. The synthetic method of the L-serine is simple in operation, short in production period, low in cost, high in yield and low in environmental protection pressure, and suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a synthesis method of amino acids, in particular to a new synthesis method of L-serine. Background technique [0002] L-Serine (L-Serine), chemically named 2-amino-3-hydroxypropionic acid, is a sugar-producing amino acid, which can be biosynthesized from glyceric acid as a precursor in the body, and threonine and glycine can also be converted into serine . Although L-serine is a non-essential amino acid, it is widely used as a biochemical reagent in food, feed, medicine, agriculture and cosmetics industries. L-serine is in the middle of amino acid metabolism and participates in the synthesis of many biological substances (such as glycine, methionine, purine, etc.), and its metabolism runs extremely fast. Therefore, compared with other types of amino acids, the production of L-serine by direct fermentation is very difficult. Due to the limitations of various technical condition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06C07C229/22C07C227/40
Inventor 姚苏吕立获何春李平袁志友彭宏涛徐新良
Owner HENGDIAN GRP JIAYUAN CHEM
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