Method for producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose

A technology of cellobiose and acetone butanol, which is applied in the field of co-fermentation of cellobiose, pentose and hexose to produce acetone butanol, to achieve the effect of reducing costs, promoting the diversification of energy structure, and ensuring the safety of liquid fuels

Inactive Publication Date: 2011-12-21
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no report on the co-fermentation of cellobiose, pentose and hexose to produce acetone butanol

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose
  • Method for producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose
  • Method for producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Co-fermentation of cellobiose, xylose and glucose mixed sugar adding organic and inorganic nitrogen and phosphorus substances

[0027] The sugar content after hydrolysis of corn stalks is: xylose 10g / L, glucose 25g / L, cellobiose 16g / L, other sugar 5g / L, total sugar 56g / L.

[0028] Medium 1: Add calcium superphosphate at 1.5% of the total sugar content, add ammonium sulfate at 1.5% of the total sugar content, add 10M / L Ca(OH) 2 Emulsion, adjusted to pH 6.0, divided into 250ml Erlenmeyer flasks, 150ml each, and sterilized in a sterilizer at 121°C for 120 minutes;

[0029] Medium 2: add calcium superphosphate according to 5% of the total sugar content, add ammonium sulfate according to 5% of the total sugar content, add 1M / L acetic acid aqueous solution, adjust the pH to 4.5, divide into 250ml Erlenmeyer flasks, each Erlenmeyer flask 150ml, sterilized in a sterilizing pot at 121°C for 120 minutes;

[0030] Medium 3: Add yeast powder according to 10% of the tot...

Embodiment 2

[0038] Embodiment 2: add corn meal fermentation in the corncob hydrolyzate

[0039] The sugar content was measured after the corncob was hydrolyzed, xylose 12g / L, glucose 27g / L, cellobiose 11g / L, other sugar 8g / L, total sugar 58g / L, add 4M / L NaOH solution to adjust the pH to 6.5 , according to the proportion of the original total sugar in the hydrolyzate of 5w%, 20w%, 50w% and 85w%, corn flour is added, so that the total sugar containing corn flour in the hydrolyzate is about 60g / L, 65g / L, 77g / L, 90g / L L, gelatinized at 100°C for 1 hour, then packed into 250ml Erlenmeyer flasks, each Erlenmeyer flask was filled with 150ml. Sterilize at 121°C for 120 minutes in a sterilizer.

[0040] After sterilization, the culture medium was taken out and cooled to room temperature, and ATCC 51743 and CICC8012 strains were inserted into the culture medium with an inoculum size of 5%, and fermented in an anaerobic incubator at 41° C. for 112 hours.

[0041] After the fermentation is complete...

Embodiment 3

[0046] Example 3: Adding organic additives to co-fermentation in cellobiose, xylose and glucose mixed sugar

[0047] In example 2 hydrolyzate, dropwise add 4M / l KOH aqueous solution and adjust pH to be 6.5, add organic agricultural sideline product mixture by 15w%, 20w%, 25w%, 30w% and 50w% of total sugar content in hydrolyzate, comprise bran , rice bran, bean cake powder and bean cake, etc., mix well, and pack into 250ml triangular bottles, each with 150ml. Sterilize at 121°C for 120 minutes in a sterilizer.

[0048] After sterilization, the culture medium was taken out and cooled to room temperature, and ATCC 51743 and CICC8012 strains were inoculated into the culture medium with an inoculum size of 5%, and fermented in an anaerobic incubator at 42°C for 108 hours.

[0049] After the fermentation is complete, get 1ml of fermented liquid and place it in a centrifuge tube, centrifuge at a speed of 11000 rpm for 7 minutes, and use gas chromatography to measure the contents of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose, which comprises the following steps: adding water, or supplementally adding pentaglucose and hexose, or supplementally adding starch materials into a hydrolysate, which is obtained by hydrolyzing straw and other lignocellulose materials, according to the total saccharide content in the hydrolysate to regulate the total saccharide content to 30-90g/L; after adding a nitrogen source or phosphorus source, regulating the pH value to 4.5-8.0, and sterilizing the materials by boiling to obtain boiled fermented liquor; cooling the boiled fermented liquor to 42 DEG C or below, inoculating the strain, and fermenting; and filtering the fermentation liquor, distilling, and rectifying to obtain the final products acetone, butanol and ethanol. The invention solves the technical problem in producing acetone and butanol by co-fermenting cellobiose, pentaglucose and hexose in a hydrolysate obtained by hydrolyzing lignocellulose, effectively enhances the conversion rate of saccharides obtained by hydrolyzing lignocellulose, increases the yield of the products acetone and butanol, and can lower the cost for producing acetone and butanol from straw to a large degree.

Description

technical field [0001] The invention relates to a method for producing acetone-butanol by fermentation, in particular to a method for producing acetone-butanol by co-fermentation of cellobiose, pentose and hexose obtained by hydrolysis of lignocellulosic raw materials. technical background [0002] Acetone butanol is an important organic solvent and chemical raw material, widely used in spray paint, explosives, plastics, pharmaceuticals, plant extraction, organic glass, synthetic rubber and other industries. Bio-butanol, similar to ethanol, is a bioprocessed alcohol fuel and a new generation of liquid fuel. The main component in gasoline is C 6 ~C 8 , so butanol is more similar to "oil" than ethanol, and has better compatibility with fuel additives and lubricating oils. Another advantage of butanol is that it can be blended with gasoline in higher concentrations than ethanol without retrofitting existing vehicles. Although both ethanol and butanol are alcohols, butanol h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/28C12P7/16C12P7/10C12R1/145
CPCY02E50/16Y02E50/10
Inventor 熊莲陈新德唐绿蓉潘微丁飞
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap