A compound premix for pigs that can improve immune function
A compound premix and premix technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of insignificant probiotic effect, limited application effect, intolerance to antibiotics, etc., to reduce morbidity and high survival rate. , the effect of promoting animal growth
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Embodiment 13
[0012] Stress resistance and biological performance measurement of embodiment 13 strain strains
[0013] 3 strains: Lactobacillus acidophilus CGMCC No.5093, Bacillus licheniformis CGMCC No.5094, Saccharomyces cerevisiae CGMCC No.2388 were isolated and bred by the applicant from the intestinal tract or feces of healthy animals, and stored in a 4°C refrigerator middle.
[0014] 1) Preparation of the culture of Bacillus licheniformis CGMCC No.5094: inoculate the slant bacteria stored in the refrigerator into BPY seed medium for activation, and cultivate at 37° C. and 200 rpm for 18 hours to obtain a culture with a spore rate of more than 95%;
[0015] 2) Preparation of the culture of Lactobacillus acidophilus CGMCC No.5093: inoculate the slant bacteria stored in the refrigerator into the MRS seed medium for activation, and culture at 35°C and 100rpm for 16 hours to obtain the product;
[0016] 3) Preparation of Saccharomyces cerevisiae CGMCC No.2388 culture: inoculate the slant ...
Embodiment 2
[0046] Embodiment 2, the preparation of compound probiotics
[0047] 1. Preparation of Bacillus licheniformis CGMCC No.5094 bacterial powder
[0048] 1) Plate culture rejuvenation: Inoculate the Bacillus licheniformis strain on the BPY plate medium, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Incubate at 37°C for 36 hours;
[0049] 2) Preparation of first-class seeds: transfer the Bacillus licheniformis cultured in step 1) to eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the late logarithmic period to obtain first-class seeds;
[0050] Described slant medium is BPY solid medium;
[0051] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is 2...
Embodiment 3
[0072] The preparation of embodiment 3 composite premixes
[0073] Contain ferrous sulfate 30g, copper sulfate 10g, zinc sulfate 12g, manganese sulfate 2g, 1% potassium iodide 0.10g, cobalt chloride 0.30g, 1% sodium selenite 0.10g, organic iron 15g, Organic copper 5g, organic zinc 6g, organic manganese 3g, vitamin A0.30g, vitamin D 3 4g, Vitamin E 3.2g, Vitamin K 3 0.18g, vitamin B 1 0.30g, vitamin B 2 0.40 Vitamin B 6 0.15, vitamin B 120.002g, pantothenic acid 1.5g, niacin 0.10g, folic acid 0.05g, biotin 0.0102g, lysine 140g, compound probiotics 100g, ethoxyquinoline 3g, 50% choline chloride 80g, zeolite powder 200g , bran supplemented to 1000g.
[0074] The preparation method of the raw material: firstly premix the vitamins and trace elements with a mixer, and then uniformly mix according to the order of "partial carrier, premixed trace element, vitamin, compound microecological preparation, remaining carrier" That's it. The preparation of embodiment 4 composite prem...
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