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Enzyme method for preparing gelatin

An enzymatic preparation and gelatin technology, applied in the field of enzymatic preparation of bone gelatin, can solve the problems of waste water discharge, unstable product quality and high extraction temperature

Active Publication Date: 2013-09-04
安徽丰原集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic gelation technology described in this patent is still affected by the particle size of the aggregate, resulting in a long enzymolysis time (enzymolysis needs to be treated overnight), high extraction temperature is easy to hydrolyze the gelatin solution again, and has a great impact on the freezing force of the product. , viscosity and other quality indicators have a greater destructive effect, because the product quality is unstable due to the need to extract the glue in batches, it is difficult to carry out industrial production, etc.
[0011] Chinese patent application 02104329.9 discloses the use of membrane technology to replace the diatomite filtration process, high-temperature evaporation concentration process and high-temperature sterilization process in the traditional gelatin preparation process. The gel-making technology described in this patent belongs to post-extraction new equipment technology, not to Research scope of enzymatic gel preparation
The enzymatic gelation technology described in this patent still needs to pickle the aggregates to obtain bone before enzymatically preparing gelatin. Because pickling is still required, a large amount of waste water will be discharged. If it is applied in actual production It still needs to be equipped with environmental protection facilities with a certain processing capacity, which is very uneconomical in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: enzymatic method gets bone gelatin

[0117] 1) Preparation and beating of bone meal:

[0118] Dry bone grains (water content≤10%, oil content≤4%), remove bone grains larger than 15cm, and then put the bone grains into a coarse grinder to grind them into bone powder with a particle size of ≤3mm (wherein bone powder with a particle size of ≤2mm accounts for 90%);

[0119] Add drinking tap water to the bone meal mixing tank G1 (self-designed, paddle agitator), add the measured bone powder into the bone meal mixing tank, and fully stir, according to the weight ratio of bone meal and water is 1:3.5 to make 22 % bone meal slurry;

[0120] 2) Colloid mill grinding (high-shear colloid mill, model HSC-100R; Shanghai Yilu Electromechanical Equipment Co., Ltd.): Add the prepared bone powder slurry into the colloid mill and grind it into a bone powder colloid solution with a particle size of 40 μm;

[0121] 3) Phosphoric acid acidification: add phosphoric acid with...

Embodiment 2

[0135] Embodiment 2: enzymatic method gets bone gelatin

[0136] Concrete steps are with embodiment 1, and following part is slightly different:

[0137] 1) Phosphoric acid acidification: start the acidification stirring, heat up the collagen colloid, and raise the temperature of the collagen colloid to 45°C;

[0138] 2) Protease hydrolysis: start stirring, and slowly add the prepared 5% acidic protease solution. The weight ratio of acid protease solution to bone powder is 0.0144:1, heat preservation, stirring, and enzymolysis for 1.5 hours.

[0139] Under the condition of constant stirring, add 0.75% hydrogen peroxide (concentration: 27.5%) of the weight of bone powder to oxidize for 8 hours, and add the same amount of hydrogen peroxide (concentration: 27.5%) for the second time to oxidize for 24 hours. If time permits, it should be extended as much as possible. oxidation time;

[0140] 3) Flocculation: start stirring, slowly add calcium hydroxide suspension, stop adding c...

Embodiment 3

[0143] Embodiment 3: enzymatic method gets bone gelatin

[0144] Concrete steps are with embodiment 1, and following part is slightly different:

[0145] 1) Phosphoric acid acidification: start the acidification stirring, heat up the collagen colloid, and raise the temperature of the collagen colloid to 50°C;

[0146] 2) Enzymatic hydrolysis and decolorization: start stirring, slowly add the prepared 5% acid protease solution, add the amount of acid protease solution according to the weight ratio of acid protease solution and bone meal is 0.027:1, keep warm, stir, enzymolysis 1 Hour;

[0147] Under the condition of constant stirring, add 0.75% hydrogen peroxide (concentration: 27.5%) of the weight of bone powder to oxidize for 8 hours, and add the same amount of hydrogen peroxide (concentration: 27.5%) for the second time to oxidize for 24 hours. If time permits, it should be extended as much as possible. oxidation time;

[0148] 3) Start stirring, slowly add the calcium hydr...

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Abstract

The invention relates to an enzyme method for preparing gelatin, which comprises the following steps of: pulverizing bone grains into bone meal, acidifying the bone meal by using phosphoric acid, and adding acid protease to hydrolyze; then, decoloring the solution by using hydrogen peroxide, and adjusting the pH value by using calcium hydroxide; and finally adding a flocculating agent. In the method provided by the invention, phosphoric acid is adopted for acidification, because a great amount of soluble calcium salts is formed, the enzymolysis reaction is mild. The defect that the enzymolysis end point of the finely ground collagen (ossein) enzymolysis method is difficult to control is overcome by controlling the end point of the enzymolysis reaction, so that the enzymolysis method for preparing gelatin is more practical.

Description

technical field [0001] The invention relates to gelatin preparation technology, in particular to a method for enzymatically preparing bone gelatin. Background technique [0002] Gelatin is a protein made from animal skin or bone. It is divided into skin gelatin and bone gelatin. Because the market demand for medicine, food and new functional gelatin is gradually expanding, with the segmentation of the gelatin market, the quality requirements for gelatin Further showing high standards, systematization and specialization. [0003] There are currently two types of bone gelatin preparation methods, one is the alkaline method, and the other is the enzymatic method. [0004] Traditional alkaline method: [0005] The traditional alkaline method of producing gelatin is to use calcium hydroxide as a collagen peptide bond breaker to partially hydrolyze the collagen, and the treated collagen is extracted with water and post-treated at a lower temperature. The process flow chart is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C09H3/00
Inventor 魏连波吴本礼齐万林卜盛永
Owner 安徽丰原集团有限公司
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