Sichuan pickled vegetable leafy vegetables fermentation compound fungus-agent and its preparing method
A technology for fermenting complex bacteria and Sichuan pickles, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low pertinence of vegetable raw materials and single bacteria, shorten the fermentation time and eliminate the influence , Guarantee the effect of quality
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Embodiment 1
[0013] The invention provides a Sichuan pickle leafy vegetable fermentation composite bacterial agent and a preparation method thereof. This microbial inoculum agent comprises bacterium powder 2 and bacterium powder 3, and its preparation method is:
[0014] Production of bacteria powder 2: 10L of culture medium of Leuconostoc mesenteroides CGMCC 1.20 strain (Leuconostoc mesenteroides CGMCC 1.20, purchased from China Common Microorganism Culture Collection Management Center) in the mid-logarithmic growth phase was inserted into a 1000L fermenter for cultivation. : 1% whey powder, 1.83% corn steep liquor, 2.35% corn flour saccharification solution, 1% peptone, 0.058% magnesium sulfate, 0.025% manganese sulfate, 0.20% sodium acetate, 0.20% dipotassium hydrogen phosphate, initial pH5. 8 After fermentation at 27°C and 48 hours of fermentation, the density of bacteria in the culture medium can reach 1.73~2.15×10 9 CFU / mL. The fermented liquid is filtered through a 100-mesh filter...
Embodiment 2
[0019]Dissolve 20-40 grams of fermented complex bacterial agent of Sichuan pickles and leafy vegetables in 100ml of tap water, dissolve for 20 minutes, add to a pickle pond filled with 2000kg of tap water, stir evenly, adjust the salt concentration to 5-7.00%, and then add 1000kg Wash fresh green vegetables, 2 kg of washed garlic, 2 kg of washed ginger and 0.5 kg of Zanthoxylum bungeanum, ferment for 25-45 days at natural temperature in closed ponds, open the ponds to test the quality of fermented pickled vegetables. The production shows that the pickled green vegetables fermented by Sichuan pickled vegetable leaf vegetable fermentation compound bacteria have bright yellow color, luster, strong sour aroma, and pure and natural fermentation aroma; the residual sugar of fermented green vegetables is less than 0.15%, and the total acidity (in terms of lactic acid) is 0.8-1.2%, amino acid nitrogen is greater than 0.30%, product yield is 65-70%, nitrite content is less than 5.50mg / k...
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