Sichuan pickled vegetable leafy vegetables fermentation compound fungus-agent and its preparing method

A technology for fermenting complex bacteria and Sichuan pickles, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low pertinence of vegetable raw materials and single bacteria, shorten the fermentation time and eliminate the influence , Guarantee the effect of quality

Inactive Publication Date: 2012-06-13
XIHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the above fermented bacterial agents are single in bacteria or the vegetable raw materials are not highly targeted, there are certain differences in the flavor of fermented pickles compared with traditional Sichuan pickles, so they are subject to certain limitations in application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The invention provides a Sichuan pickle leafy vegetable fermentation composite bacterial agent and a preparation method thereof. This microbial inoculum agent comprises bacterium powder 2 and bacterium powder 3, and its preparation method is:

[0014] Production of bacteria powder 2: 10L of culture medium of Leuconostoc mesenteroides CGMCC 1.20 strain (Leuconostoc mesenteroides CGMCC 1.20, purchased from China Common Microorganism Culture Collection Management Center) in the mid-logarithmic growth phase was inserted into a 1000L fermenter for cultivation. : 1% whey powder, 1.83% corn steep liquor, 2.35% corn flour saccharification solution, 1% peptone, 0.058% magnesium sulfate, 0.025% manganese sulfate, 0.20% sodium acetate, 0.20% dipotassium hydrogen phosphate, initial pH5. 8 After fermentation at 27°C and 48 hours of fermentation, the density of bacteria in the culture medium can reach 1.73~2.15×10 9 CFU / mL. The fermented liquid is filtered through a 100-mesh filter...

Embodiment 2

[0019]Dissolve 20-40 grams of fermented complex bacterial agent of Sichuan pickles and leafy vegetables in 100ml of tap water, dissolve for 20 minutes, add to a pickle pond filled with 2000kg of tap water, stir evenly, adjust the salt concentration to 5-7.00%, and then add 1000kg Wash fresh green vegetables, 2 kg of washed garlic, 2 kg of washed ginger and 0.5 kg of Zanthoxylum bungeanum, ferment for 25-45 days at natural temperature in closed ponds, open the ponds to test the quality of fermented pickled vegetables. The production shows that the pickled green vegetables fermented by Sichuan pickled vegetable leaf vegetable fermentation compound bacteria have bright yellow color, luster, strong sour aroma, and pure and natural fermentation aroma; the residual sugar of fermented green vegetables is less than 0.15%, and the total acidity (in terms of lactic acid) is 0.8-1.2%, amino acid nitrogen is greater than 0.30%, product yield is 65-70%, nitrite content is less than 5.50mg / k...

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PUM

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Abstract

The invention discloses a Sichuan pickled vegetable leafy vegetables fermentation compound fungus-agent and its preparing method, the microbe fungus-agent comprises bacterium powder 2 and bacterium powder 3. The preparation method comprises the following steps: 1) bacterium powder 2 preparation, 10L of Leuconostoc mesenteroide CGMCC 1.20 bacterial strain nutrient solution with logarithm proliferation is introduced in a fermentation tank with volume of 1000 L for culture; 2) bacterium powder 3 preparation, 5L of logarithm proliferation Lactobacillus plantarum CICC 6067 nutrient solution, 5L of lactobacillus brevis CICC 6042 nutrient solution and Pediococcus acidilactici CGMCC 1.4 nutrient solution are respectively introduced in the fermentation tank with volume of 1000 L for co-culture; mixing the bacterium powder 2 and the bacterium powder 3 according to ratio of 1:1(weight), vacuum packaging to obtain the Sichuan pickled vegetable leaf vegetable fermentation compound fungus-agent. The fermentation time can be shortened, the uniform quality of the fermented pickled vegetable can be ensured, the influence of natural environment on fermentation can be eliminated, and traditional pickled vegetable local flavor can be reproduced, compared with the traditional Sichuan pickled vegetable, the fermented Sichuan pickled vegetable has no obvious difference on local flavor.

Description

technical field [0001] The invention relates to a Sichuan pickle leafy vegetables fermentation composite bacterial agent and a preparation method thereof. Background technique [0002] Kimchi is a kind of vegetable food made from various fresh vegetables and fermented by lactic acid bacteria in a low-salt solution. Sichuan is one of the main producing areas of pickles in my country. Sichuan kimchi is famous for its fresh and pure acidity, crisp and tender aroma, long aftertaste, and appetizing. It can not only be eaten as a side dish, but also an indispensable seasoning dish for Sichuan cuisine, known as "the bone of Sichuan cuisine". [0003] "The world's kimchi looks at China, and the Chinese kimchi looks at Sichuan." Sichuan kimchi is a typical representative of kimchi in my country. Its production history can be traced back to the Shang and Zhou Dynasties 3,000 years ago. ". In recent years, with the improvement of people's understanding of the biological function an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/218C12N1/20C12R1/01C12R1/25C12R1/24C12R1/46A23L19/20
Inventor 向文良高银江车振明李明元邢亚阁张琦龚丽田伟李可王嫚
Owner XIHUA UNIV
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